NGS in the clinical microbiology settings

Pitashny, Milena; Kadry, Balqees; Shalaginov, Raya; Gazit, Liat; Zohar, Yaniv; Szwarcwort, Moran; Stabholz, Yoav

doi:10.3389/fcimb.2022.955481

Cited by 7 publications

(6 citation statements)

References 9 publications

(13 reference statements)

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“…Beside LA, a variety of technologies are revolutionizing clinical microbiology. These include MALDI-TOF MS ( Seng et al., 2009 ), time-lapse microscopy for ID and phenotypic AST ( Charnot-Katsikas et al., 2018 ), molecular diagnostic tests and syndromic panels ( Arena et al., 2017 ; Trotter et al., 2019 ), and next-generation sequencing ( Mitchell and Simner, 2019 ; Pitashny et al., 2022 ). All of them can significantly improve the diagnosis and therapy of infections, but as stated above, they are primarily complementary to culture ( Trotter et al., 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Laboratory automation, informatics, and artificial intelligence: current and future perspectives in clinical microbiology

Mencacci,

De Socio,

Pirelli

et al. 2023

Front. Cell. Infect. Microbiol.

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Clinical diagnostic laboratories produce one product—information—and for this to be valuable, the information must be clinically relevant, accurate, and timely. Although diagnostic information can clearly improve patient outcomes and decrease healthcare costs, technological challenges and laboratory workflow practices affect the timeliness and clinical value of diagnostics. This article will examine how prioritizing laboratory practices in a patient-oriented approach can be used to optimize technology advances for improved patient care.

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Section: Discussionmentioning

confidence: 99%

Laboratory automation, informatics, and artificial intelligence: current and future perspectives in clinical microbiology

Mencacci,

De Socio,

Pirelli

et al. 2023

Front. Cell. Infect. Microbiol.

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“…This outcome underscores the utility of mNGS analysis in pathogen identification. 14 Therefore, when an infection involving multiple microorganisms is suspected, mNGS analysis should be prioritized.…”

Section: Declaration Of Conflicting Interestmentioning

confidence: 99%

Porphyromonas gingivalis-induced hematogenous disseminated severe pneumonia: a case report

Yuanjun,

Yan,

Qingyan

et al. 2024

J Int Med Res

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Porphyromonas gingivalis, a gram-negative oral anaerobe among more than more than 500 bacterial species that colonizing the oral cavity, is involved in the pathogenesis and prototypic polybacterial consortium of periodontitis. It is mainly found in oral infections and rarely present in other organ diseases. Here, we describe a 43-year-old man with underlying diabetes who developed hematogenous disseminated severe pneumonia after P. gingivalis had invaded the blood. Next-generation sequencing of early alveolar lavage fluid and blood samples confirmed the diagnosis. The patient’s lung infection improved after targeted antimicrobial treatment. He was successfully weaned from ventilatory support and transferred to the general ward. This case illustrates bacterial entry into the bloodstream of a patient with diabetes who had periodontal disease but did not maintain oral hygiene, leading to severe pneumonia. Periodontal disease is often ignored by the public, and it is difficult for critical care physicians to link severe pneumonia with periodontal disease. Thus, this case represents an important warning to critical care clinicians.

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“…Also, in the realm of thoracic oncology, Polymerase Chain Reaction (PCR) and Next-Generation Sequencing (NGS) studies serve as prominent tools for identifying cancer cells and deciphering the genetic alterations driving cancer growth [17,18]. PCR-based techniques exhibit exceptional efficacy in detecting minute quantities of circulating tumor DNA (ctDNA), facilitating the early identification of tumor presence.…”

Section: Investigational Frontiers: Distinguishing Pcr-based and Ngs-...mentioning

confidence: 99%

Innovations in Thoracic Oncology and the Promise of Liquid Biopsies with Dr. Luis Raez

Cortiana,

Van de Kieft,

Chorya

et al. 2024

Cancers

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Thoracic oncology continues to pose a great threat to human health as one of the most common forms of cancer. Liquid biopsies present a transformative approach to treating patients affected by these types of diseases by providing a less invasive genetic overview of the tumor, aiding in both diagnostic and treatment measures. The primary objective of this article is to examine the prospects of liquid biopsies in managing thoracic malignancies and to present barriers to their usage as demonstrated by Dr. Luis Raez. In examining why molecular diagnostics continue to be employed together with more traditional methods, this article presents the next steps in the clinical application of blood-based cancer screening. Future cancer diagnosis and treatment aim to prioritize circulating biomarker analyses based on their potential for the detection and monitoring of thoracic cancers. Liquid biopsies are favored thanks to their reduced invasiveness with respect to traditional treatments. The further study of clinical biomarkers and technological advancements are thus pivotal to enhance the clinical applicability of this method. In conclusion, this blood-based analysis offers a promising route by which the diagnosis, treatments, and outcomes of thoracic cancer can be improved.

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NGS in the clinical microbiology settings

Cited by 7 publications

References 9 publications

Laboratory automation, informatics, and artificial intelligence: current and future perspectives in clinical microbiology

Laboratory automation, informatics, and artificial intelligence: current and future perspectives in clinical microbiology

Porphyromonas gingivalis-induced hematogenous disseminated severe pneumonia: a case report

Innovations in Thoracic Oncology and the Promise of Liquid Biopsies with Dr. Luis Raez

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