NGFI-C expression is affected by physiological stimulation and seizures in the somatosensory cortex

Mack, Kenneth J.; Yi, Sang-Doe; Chang, Shu-Ying; Millan, Noemi; Mack, Pat

doi:10.1016/0169-328x(94)00243-8

Cited by 26 publications

(8 citation statements)

References 22 publications

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“…We have demonstrated widespread expression of Egr-3 protein in the rat brain in regions including the cerebral cortex, especially layers II and IV, caudate putamen, hippocampus, thalamus, inferior colliculus, cerebellar Purkinje cells, and brainstem nuclei. The distribution of Egr-3 immunoreactivity in cor-tical layers is similar to that reported by Mack et al (1995).…”

Section: Regional Specificity Of Egr Dna-binding Activitysupporting

confidence: 87%

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Differential Expression of Egr‐1‐Like DNA‐Binding Activities in the Naive Rat Brain and After Excitatory Stimulation

Beckmann

Davidson

Goodenough

et al. 1997

Journal of Neurochemistry

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Egr-1 and related proteins are inducible transcription factors within the brain recognizing the same consensus DNA sequence. Three Egr DNA-binding activities were observed in regions of the naive rat brain. Egr-1 was present in all brain regions examined. Bands composed, at least in part, of Egr-2 and Egr-3 were present in different relative amounts in the cerebral cortex, striatum, hippocampus, thalamus, and midbrain. All had similar affinity and specificity for the Egr consensus DNA recognition sequence. Administration of the convulsants NMDA, kainate, and pentylenetetrazole differentially induced Egr-1 and Egr-2/3 DNA-binding activities in the cerebral cortex, hippocampus, and cerebellum. All convulsants induced Egr-1 and Egr-2 immunoreactivity in the cerebral cortex and hippocampus. These data indicate that the members of the Egr family are regulated at different levels and may interact at promoters containing the Egr consensus sequence to fine tune a program of gene expression resulting from excitatory stimuli.

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Section: Regional Specificity Of Egr Dna-binding Activitysupporting

confidence: 87%

“…1995;Mack et al, 1995) also does not exclude a role for this protein in the Egr DNA-binding activities observed in this study.…”

Section: Regional Specificity Of Egr Dna-binding Activitymentioning

confidence: 66%

Differential Expression of Egr‐1‐Like DNA‐Binding Activities in the Naive Rat Brain and After Excitatory Stimulation

Beckmann

Davidson

Goodenough

et al. 1997

Journal of Neurochemistry

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“…Multiplex assays, in which the gene of interest is amplified together with an internal (i.e. housekeeping gene) or external control (in vitro transcribed RNA) of different size [22,23], and competitive PCR, in which the target DNA is competing for amplifcation with a standard of known concentration [24], have successfully been employed to overcome this problem. Recent development of a fluorogenic system now allows continuous quantification of PCR products (including internal controls) as the PCR proceeds, providing an effective tool for routine quantification of mRNAs present in widely differing abundance.…”

Section: Detecting Changes In Gene Expressionmentioning

confidence: 99%

Memory formation and the regulation of gene expression

Stork

Welzl

1999

Cellular and Molecular Life Sciences (CMLS)

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On a cellular level, formation of memory is based on a selective change in synaptic efficacy that is both fast and, in case of important information, long-lasting. Rapidity of cellular changes is achieved by modifying preexisting synaptic molecules (receptors, ion channels), which instantaneously alters the efficacy of synaptic transmission. Endurance, that is the formation of long-term memory (LTM), is based on transient and perhaps also long-lasting changes in protein synthesis. A number of different methods exist to interfere with the synthesis of specific proteins or proteins in general. Other methods, in turn, help to identify proteins whose synthesis is changed following learning. These mostly molecular methods are briefly described in the present review. Their successful application in a variety of memory paradigms in invertebrates and vertebrates is illustrated. The data support the importance of selective changes in gene expression for LTM. Proteins newly synthesized during memory consolidation are likely to contribute to restructuring processes at the synapse. altering the efficiency of transmission beyond the scope of STM. Increased or, less often, decreased synthesis of proteins appears during specific time windows following learning. Recent evidence supports older data suggesting that two or even more waves of protein synthesis exist during the consolidation period. It is expected that the new molecular methods will help to identify and characterize molecules whose expression changes during LTM formation even in complex vertebrate learning paradigms.

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“…Results were normalized to those of ␤-actin. The primers for Egr-1, Egr-3, and NGFI-C have been described previously (23,24).…”

Section: Methodsmentioning

confidence: 99%

Activation of Serum Response Factor in the Depolarization Induction of Egr-1 Transcription in Pancreatic Islet β-Cells

Bernal-Mizrachi

Wice

Inoue

et al. 2000

Journal of Biological Chemistry

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The results of the current studies define the major elements whereby glucose metabolism in islet ␤-cells leads to transcriptional activation of an early response gene in insulinoma cell lines and in rat islets. Glucose stimulation (2-20 mM) resulted in a 4-fold increase in Egr-1 mRNA at 30 min, as did the depolarizing agents KCl and tolbutamide. This response was inhibited by diazoxide and EGTA, indicating that ␤-cell depolarization and Ca Pancreatic islet ␤-cell mass can be regulated by multiple stimuli, including growth factors and nutrients. The ability to produce insulin is proportional to the islet mass that is defined by a balance between rates of proliferation and apoptosis. Several growth factors have been shown to stimulate pancreatic

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NGFI-C expression is affected by physiological stimulation and seizures in the somatosensory cortex

Cited by 26 publications

References 22 publications

Differential Expression of Egr‐1‐Like DNA‐Binding Activities in the Naive Rat Brain and After Excitatory Stimulation

Differential Expression of Egr‐1‐Like DNA‐Binding Activities in the Naive Rat Brain and After Excitatory Stimulation

Memory formation and the regulation of gene expression

Activation of Serum Response Factor in the Depolarization Induction of Egr-1 Transcription in Pancreatic Islet β-Cells

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