NGF stimulates incorporation of fucose or glucosamine into an external glycoprotein in cultured rat PC12 pheochromocytoma cells

McGuire, Jeffrey C.; Greene, Lloyd A.; Furano, Anthony V.

doi:10.1016/0092-8674(78)90004-1

Cited by 173 publications

(90 citation statements)

References 35 publications

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“…In the presence of nerve growth factor (NGF), PC12 cells acquire neuronal characteristics such as the outgrowth of neurites and electrical excitability, suggesting that this system may facilitate the evaluation of changes in biochemical composition that accompany the NGF-induced differentiation of these cells (1,2). Specific protein and gene expression profiles during the differentiation process of PC12 cells have been determined by various methods such as two-dimensional gel electrophoresis of cellular proteins and the differential screening of cDNA libraries (3)(4)(5). The information obtained by these methods, however, is somewhat limited.…”

Section: Introductionmentioning

confidence: 99%

Identification and initial characterization of stathmin by the differential display method in nerve growth factor-treated PC12 cells

Takekoshi

Nomura²,

Isobe³

et al. 1998

European Journal of Endocrinology

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The differential display of mRNA is a new strategy to identify genes that are differentially expressed under altered conditions. We applied this method to determine differential gene expression in the rat pheochromocytoma cell line during differentiation induced by nerve growth factor (NGF).Three different mRNA species were isolated, and their differential expression was confirmed by RT-PCR. One of the mRNA species was identified as stathmin, a 19 kDa cytosolic protein attracting increasing interest for its role in signal transduction. In the NGF-treated PC12 cells, the expression of stathmin mRNA increased in a time-dependent manner, as assessed by northern blot analysis and RT-PCR. We also assessed by northern blot analysis how the expression of stathmin mRNA was altered in human pheochromocytomas (n ¼ 5) compared with that in normal adrenal medulla tissue (n ¼ 5). The mRNA concentrations were found to be significantly greater in the pheochromocytomas than in the normal tissues. It has been shown that stathmin mRNA concentrations are increased in various tumor cells. As pheochromocytomas are well-differentiated tumors of neural origin, it is not unexpected that stathmin mRNA is overexpressed in these tumors.Stathmin was isolated and identified as a differentially expressed gene by the differential display method in PC12 cells during differentiation induced by NGF. In addition, stathmin mRNA was found to be overexpressed in human pheochromocytomas. The mechanisms responsible for the up-regulation of stathmin mRNA during differentiation of PC12 cells and the significance of its overexpression in human pheochromocytomas remain to be determined.

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Section: Introductionmentioning

confidence: 99%

Identification and initial characterization of stathmin by the differential display method in nerve growth factor-treated PC12 cells

Takekoshi

Nomura²,

Isobe³

et al. 1998

European Journal of Endocrinology

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“…We have been interested in a family of glycoproteins found on the surfaces of rat and mouse neurons. The best characterized of these glycoproteins is the 230,000-dalton nerve growth factor-inducible large external (NILE) glycoprotein which is expressed on the surface of PC12 cells (McGuire et al, 1978;Lee et al, 1981;Salton et al, 1983a, b).…”

Section: Introductionmentioning

confidence: 99%

Antibody against nerve growth factor-inducible large external (NILE) glycoprotein labels nerve fiber tracts in the developing rat nervous system

Stallcup¹,

Beasley²,

Levine³

1985

J. Neurosci.

125

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The NILE (nerve growth factor-inducible large external) glycoprotein is a 230,000-dalton molecule found on the surface of PC12 cells. immunologically cross-reactive glycoproteins in the molecular weight range of 215,000 to 230,000 have been found on many types of neurons in culture. Using immunohistochemical methods, we have shown that NILErelated glycoproteins are present in neuronal fiber tracts of the developing rat brain. Antibody against the NILE glycoprotein specifically labels processes that appear identical to those recognized by antibodies against the neurofilament triplet of proteins. These processes are clearly distinct from the radial glial fibers recognized by antibody against the intermediate filament protein vimentin. NILE glycoprotein is not distributed uniformly over the entire neuronal surface but is concentrated on neurites and is much less abundant on cell bodies. NILE-positive fiber tracts are first seen in the spinal cord and rhombencephalon on embryonic day 11 and over the next 2 days appear in the mesencephalon and diencephalon.Staining in the telencephalon is not seen until embryonic day 15. The appearance of NILE immunoreactivity in these various regions closely parallels the appearance of neurofilament polypeptides, suggesting that NILE-related glycoproteins are present during the early phases of fiber tract formation. This idea is supported by the finding that the NILE glycoprotein can be found postnatally in parts of the nervous system such as the cerebellar cortex and olfactory bulb which undergo major histogenesis during the postnatal period. In the cerebellum the appearance of NILE immunoreactivity in the two major fiber zones, the molecular layer and the white matter, parallels the development of the fiber structure of these layers. These findings support tissue culture studies which suggest a role for the NILE glycoprotein in mediating nerve fiber fasciculation.

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“…In the presence of NGF, PC12 cells differentiate from adrenal chromaffin-like cells into sympathetic neuronlike cells. A number of gene products have been identified that are quantitatively induced during the neural differentiation of PC12 cells, including the NGF-inducible large external glycoprotein (66,85,86), ornithine decarboxylase (ODC) (33,65), SCG10 (3,4), GAP-43 (10,49), VGF (57), peripherin (55,56), and the protease transin (63). In addition, several immediate-early genes, some of which encode transcriptional regulatory proteins, are rapidly turned on in PC12 cells in response to NGF or epidermal growth factor (EGF) (21,22,25,35,52,69,70,98,108).…”

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confidence: 99%

Structure of the gene encoding VGF, a nervous system-specific mRNA that is rapidly and selectively induced by nerve growth factor in PC12 cells.

1991

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Nerve growth factor (NGF) plays a critical role in the development and survival of neurons in the peripheral nervous system. Following treatment with NGF but not epidermal growth factor, rat pheochromocytoma (PC12) cells undergo neural differentiation. We have cloned a nervous system-specific mRNA, NGF33.1, that is rapidly and relatively selectively induced by treatment of PC12 cells with NGF and basic fibroblast growth factor in comparison with epidermal growth factor. Analysis of the nucleic acid and predicted amino acid sequences of the NGF33.1 cDNA clone suggested that this clone corresponded to the NGF-inducible mRNA called VGF (A. Levi, J. D. Eldridge, and B. M. Paterson, Science 229:393-395, 1985; R. Possenti, J. D. Eldridge, B. M. Paterson, A. Grasso, and A. Levi, EMBO J. 8:2217-2223, 1989). We have used the NGF33.1 cDNA clone to isolate and characterize the VGF gene, and in this paper we report the complete sequence of the VGF gene, including 853 bases of 5' flank revealed TATAA and CCAAT elements, several GC boxes, and a consensus cyclic AMP response element-binding protein binding site. The VGF promoter contains sequences homologous to other NGF-inducible, neuronal promoters. We further show that VGF mRNA is induced in PC12 cells to a greater extent by depolarization and by phorbol-12-myristate-13-acetate treatment than by 8-bromo-cyclic AMP treatment. By Northern (RNA) and RNase protection analysis, VGF mRNA is detectable in embryonic and postnatal central and peripheral nervous tissues but not in a number of nonneural tissues. In the cascade of events which ultimately leads to the neural differentiation of NGF-treated PC12 cells, the VGF gene encodes the most rapidly and selectively regulated, nervous-system specific mRNA yet identified.

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NGF stimulates incorporation of fucose or glucosamine into an external glycoprotein in cultured rat PC12 pheochromocytoma cells

Cited by 173 publications

References 35 publications

Identification and initial characterization of stathmin by the differential display method in nerve growth factor-treated PC12 cells

Identification and initial characterization of stathmin by the differential display method in nerve growth factor-treated PC12 cells

Antibody against nerve growth factor-inducible large external (NILE) glycoprotein labels nerve fiber tracts in the developing rat nervous system

Structure of the gene encoding VGF, a nervous system-specific mRNA that is rapidly and selectively induced by nerve growth factor in PC12 cells.

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