NGF regulates sertoli cell growth and prevents LPS-induced junction protein damage via PI3K/AKT/NFκB signaling

Lv, Dong-Liang; Zhao, Mengjie; Ni, Jie; Liu, Weidong; Ren, Yijie; Zhu, Dawei; Hu, Jianhong

doi:10.1016/j.theriogenology.2022.10.017

Cited by 6 publications

(2 citation statements)

References 55 publications

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“…The nerve growth factor receptor is expressed on the surface of SCs and increases their survival. This factor also restores the expression of the zonula occludens-1, Ocln, Gja1, and Cdh2 genes and the dynamics of BTB after testicular injury (29). The upregulation of gene expression in the present study could be due to the presence of these factors in SCCM.…”

Section: Real-time Pcr Analysissupporting

confidence: 60%

Sertoli cell-conditioned medium can improve blood-testis-barrier function and spermatogenesis in azoospermia mice induced by scrotal hyperthermia: An experimental study

Aghajanpour,

Soltani,

Afshar

et al. 2024

IJRM

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Background: An increase in the temperature of the testis is associated with damage to the epithelium of seminiferous tubules and disruption of sperm production. Objective: The current study aimed to investigate the effect of the Sertoli cell-conditioned medium (SCCM) on the blood-testis-barrier associated genes and spermatogenesis process following scrotal hyperthermia. Materials and Methods: In this experimental study, 40 adult NMRI mice (8 wk, 25–30 gr) were allocated into 4 groups: I) control, II) DMEM (10 μl Dulbecco’s Modified Eagle Medium), III) scrotal hyperthermia, and IV) scrotal hyperthermia+SCCM (10 μl SCCM). Hyperthermia was induced by placing the mice scrotum in water at 43°C for 20 min every other day for 10 days. Mice were treated every other day for 5 wk. Then the animals were euthanized, and the tails of epididymis were removed to analyze sperm parameters, testis were taken for stereological assessment, reactive oxygen spices and glutathione levels, and the expression of Ocln, Gja1, Cdh2, and Itgb1. Results: The results of sperm analysis indicated that SCCM-treated mice significantly increased sperm count and motility and reduced DNA fragmentation. In addition, histological and molecular findings showed that the volume of testicular tissue, the number of germ cells, the glutathione level, and the expression of Ocln, Gja1, Cdh2, and Itgb1 genes were significantly increased in the SCCM-treated mice. Conclusion: Findings suggest that growth factors of SCCM stimulate the proliferation and differentiation of germ cells through paracrine effects and upregulate the blood-testis-barrier-associated genes in mice subjected to scrotal hyperthermia. Key words: Testis, Sertoli cells, Culture media, Hyperthermia, Spermatogenesis.

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Section: Real-time Pcr Analysissupporting

confidence: 60%

Sertoli cell-conditioned medium can improve blood-testis-barrier function and spermatogenesis in azoospermia mice induced by scrotal hyperthermia: An experimental study

Aghajanpour,

Soltani,

Afshar

et al. 2024

IJRM

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“…Sertoli-Sertoli cell (tight) junctions must also be dynamically regulated, uncoupling to allow germ cells to migrate between the SCs, and then immediately reforming to maintain the blood-testes barrier and form interactions with the next developing germ cell (20). Mechanisms regulating junction formation and dynamics have been extensively studied (21)(22)(23); the current review will focus specifically on the role of MAPK proteins.…”

Section: Mapks Integrate Signals To Dynamically Regulate Testicular C...mentioning

confidence: 99%

MAPKs signaling is obligatory for male reproductive function in a development-specific manner

Kumar,

Solanki,

Jain

et al. 2024

Front. Reprod. Health

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Mitogen-activated protein kinases (MAPKs) represent widely expressed and evolutionarily conserved proteins crucial for governing signaling pathways and playing essential roles in mammalian male reproductive processes. These proteins facilitate the transmission of signals through phosphorylation cascades, regulating diverse intracellular functions encompassing germ cell development in testis, physiological maturation of spermatozoa within the epididymis, and motility regulation at ejaculation in the female reproductive tract. The conservation of these mechanisms appears prevalent across species, including humans, mice, and, to a limited extent, livestock species such as bovines. In Sertoli cells (SCs), MAPK signaling not only regulates the proliferation of immature SCs but also determines the appropriate number of SCs in the testes at puberty, thereby maintaining male fertility by ensuring the capacity for sperm cell production. In germ cells, MAPKs play a crucial role in dynamically regulating testicular cell-cell junctions, supporting germ cell proliferation and differentiation. Throughout spermatogenesis, MAPK signaling ensures the appropriate Sertoli-to-germ cell ratio by regulating apoptosis, controlling the metabolism of developing germ cells, and facilitating the maturation of spermatozoa within the cauda epididymis. During ejaculation in the female reproductive tract, MAPKs regulate two pivotal events—capacitation and the acrosome reaction essential for maintaining the fertility potential of sperm cells. Any disruptions in MAPK pathway signaling possibly may disturb the testicular microenvironment homeostasis, sperm physiology in the male body before ejaculation and in the female reproductive tract during fertilization, ultimately compromising male fertility. Despite decades of research, the physiological function of MAPK pathways in male reproductive health remains inadequately understood. The current review attempts to combine recent findings to elucidate the impact of MAPK signaling on male fertility and proposes future directions to enhance our understanding of male reproductive functions.

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Nerve growth factor alleviates arsenic-induced testicular injury by enhancing the function of Sertoli cells

Yang,

Hong,

Han

et al. 2024

Ecotoxicology and Environmental Safety

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NGF regulates sertoli cell growth and prevents LPS-induced junction protein damage via PI3K/AKT/NFκB signaling

Cited by 6 publications

References 55 publications

Sertoli cell-conditioned medium can improve blood-testis-barrier function and spermatogenesis in azoospermia mice induced by scrotal hyperthermia: An experimental study

Sertoli cell-conditioned medium can improve blood-testis-barrier function and spermatogenesis in azoospermia mice induced by scrotal hyperthermia: An experimental study

MAPKs signaling is obligatory for male reproductive function in a development-specific manner

Nerve growth factor alleviates arsenic-induced testicular injury by enhancing the function of Sertoli cells

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