NGF primed spleen cells injected in brain of developing rats differentiate into mast cells

Aloe, Luigi; Simone, Roberta De

doi:10.1016/0736-5748(89)90015-4

Cited by 28 publications

(11 citation statements)

References 27 publications

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“…This observation was confirmed and extended in later years by showing that NGF-primed spleen cells, transplanted into the brain ventricles of donor rats, differentiate into MC (71).…”

Section: Ngf and The Immune Systemsupporting

confidence: 51%

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The expanding role of nerve growth factor: from neurotrophic activity to immunologic diseases

Aloe

Bracci-Laudiero

Бонини

et al. 1997

Allergy

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204

127

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Numerous studies published in the last 10–15 years have shown that nerve growth factor (NGF), a polypeptide originally discovered in connection with its neurotrophic activity, also acts on cells of the immune system. NGF has been found in various immune organs including the spleen, lymph nodes, and thymus, and cells such as mast cells, eosinophils, and B and T cells. The circulating levels of NGF increase in inflammatory responses, in various autoimmune diseases, in parasitic infections, and in allergic diseases. Stress‐related events both in animal models and in man also result in an increase of NGF, suggesting that this molecule is involved in neuroendocrine functions. The rapid release of NGF is part of an alerting signal in response to either psychologically stressful or anxiogenic conditions in response to homeostatic alteration. Thus, the inflammation and stress‐induced increase in NGF might alone or in association with other biologic mediators induce the activation of immune cells during immunologic insults. A clearer understanding of the role of NGF in these events may be useful to identify the mechanisms implicated in certain neuroimmune and immune dysfunctions.

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“…This observation was confirmed and extended in later years by showing that NGF-primed spleen cells, transplanted into the brain ventricles of donor rats, differentiate into MC (71).…”

Section: Ngf and The Immune Systemsupporting

confidence: 51%

“…MC express NGF-r (70)(71)(72)(73) and in vitro administration of NGF induces MC degranulation and histamine release (70,74), increasing their survival (72,75) (Fig. 1).…”

Section: Ngf and The Immune Systemmentioning

confidence: 99%

The expanding role of nerve growth factor: from neurotrophic activity to immunologic diseases

Aloe

Bracci-Laudiero

Бонини

et al. 1997

Allergy

Self Cite

204

127

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“…NGF induces biogenic amine release from rat peritoneal mast cells3,6 and synergises with other agonists in this respect, and histamine and 5-HT in turn appear to influence cholinergic function.7 NGF is involved in the differentiation of mast cells in embryo rat brain and NGFprimed spleen cells injected into the lateral ventricles of developing rat brain differentiate into mast cells. 8 Furthermore, NGF induces a systemic increase and phenotypic change in rat tissue mast cells,9 promotes the survival of rat mast cells in ' culture10 and neurofibromas have been associated with systemic mastocytosis.ll Overall, NGF therefore appears to have potent biological activities in addition to its neurotrophic effects.…”

Section: Introductionmentioning

confidence: 99%

Human Placental Mast Cells as an In Vitro Model System in Aspects of Neuro-Immunotoxicity Testing

Purcell

Atterwill

1994

Hum Exp Toxicol

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1 In both the developing and adult nervous systems, nerve growth factor (NGF) influences neuronal survival, differentiation and recovery following insult, 2 The effect of NGF upon human placental mast cells (HPMC) was investigated, since it is known that rodent mast cells express a functional receptor for NGF and secrete histamine upon challenge with this neurotrophic factor. Furthermore, human placental tissue contains a significant amount of NGF and expresses a NGF receptor. 3 HPMC were shown to secrete histamine in a concentration dependent manner in response to NGF (0.001-10.0 μg ml-1) in the presence of the lipid cofactor phosphatidylserine (10.0 μg ml-1). 4 NGF induced histamine release from isolated HPMC with an EC50 of 0.1 μg ml -1 NGF and maximal secretion of total cellular histamine of 22.3 ± 3.4% at 3.0 μg ml-1. 5 The response was shown to be a secretory process, dependent upon the presence of exogenous calcium ions and to be pH- and temperature-sensitive. 6 HPMC are suggested to be a suitable primary cell model for use in aspects of in vitro toxicity testing, in terms of assessing the neuro-immunotoxic potential of neurotrophic therapeutics. In addition, mechanistic studies concerning those xenobiotics which may exert their neurotoxic effect via interaction with neurotrophic factors and, or their receptors, may be studied in this human cell model.

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“…The association of mucosal type mast cell with development or expression of the model disease is further supported by their localisation, close to inflamed blood vessels 13 days post immunisation. The hyperplasia of these cells could be tentatively related to the enhanced NGF and NGF mRNA (Micera et al, 1995;De Simone et al, 1996) levels in various brain areas, including brain-stem of EAE rats since NGF was shown to induce mast cell proliferation in the neonatal rat brain (Aloe and Levi-Montalcini, 1977;Aloe and De Simone, 1989;Marshall et al, 1990;LeviMontalcini et al, 1995).…”

Section: Discussionmentioning

confidence: 95%

Mast cell specific proteases in rat brain: changes in rats with experimental allergic encephalomyelitis

Rouleau

Dimitriadou²,

Tuong

et al. 1997

J. Neural Transmission

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Mast cell populations were identified within brain parenchyma by their specific proteases, using antibodies for immunohistochemistry and ELISAs, and riboprobes were developed for in situ hybridisation. Connective tissue mast cells expressing rat mast cell protease I (RMCPI) mRNA and immunoreactivity were observed in thalamus and showed no degranulation at 3, 8 and 13 days after induction of experimental allergic encephalomyelitis (EAE). Mucosal-like mast cells were clearly demonstrated in control rats by measuring RMCPII and by visualising cells expressing RMCPII mRNA and immunoreactivity. At day 13, but not 3 and 8 post immunisation, the number of RMCPII-expressing cells markedly increased in the EAE-induced group, mainly within brainstem and spinal cord close to inflammed blood vessels. The markers of histaminergic neurons were marginally affected 13 days after immunisation and the increase of [3H] histamine synthesis elicited by the H3-receptor antagonist, thioperamide, was not modified in any region of the brain. It is concluded that the cerebral RMCPII-expressing mast cells could play a role during EAE.

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NGF primed spleen cells injected in brain of developing rats differentiate into mast cells

Cited by 28 publications

References 27 publications

The expanding role of nerve growth factor: from neurotrophic activity to immunologic diseases

The expanding role of nerve growth factor: from neurotrophic activity to immunologic diseases

Human Placental Mast Cells as an In Vitro Model System in Aspects of Neuro-Immunotoxicity Testing

Mast cell specific proteases in rat brain: changes in rats with experimental allergic encephalomyelitis

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