NGEP, a gene encoding a membrane protein detected only in prostate cancer and normal prostate

Abstract

We identified a gene (NGEP) that is expressed only in prostate cancer and normal prostate. The two NGEP transcripts are 0.9 kb and 3.5 kb in size and are generated by a differential splicing event. The short variant (NGEP-S) is derived from four exons and encodes a 20-kDa intracellular protein. The long form (NGEP-L) is derived from 18 exons and encodes a 95-kDa protein that is predicted to contain seven-membrane-spanning regions. In situ hybridization shows that NGEP mRNA is localized in epithelial cells of normal prostate and prostate cancers. Immunocytochemical analysis of cells transfected with NGEP cDNAs containing a Myc epitope tag at the carboxyl terminus shows that the protein encoded by the short transcript is localized in the cytoplasm, whereas the protein encoded by the long transcript is present on the plasma membrane. Because of its selective expression in prostate cancer and its presence on the cell surface, NGEP-L is a promising target for the antibody-based therapies of prostate cancer.

International Union of Basic and Clinical Pharmacology. LXXXV: Calcium-Activated Chloride Channels

“…Suzuki et al, 2010;Castoldi et al, 2011 TMEM16G Expression in prostate cancer and normal prostate. Bera et al, 2004;Das et al, 2007 TMEM16K Mutation found in autosomal-recessive cerebellar ataxia. Vermeer et al, 2010 12 and cancer.…”

“…RNA analysis revealed two splice variants of TMEM16G mRNA: a short form encoding a soluble protein and a long form encoding a polytopic membrane protein. Overexpression of TMEM16G in the prostate cancer cell line LNCaP promotes formation of large cell aggregates (Bera et al, 2004;Das et al, 2007). However, the exact role of TMEM16G in prostate is unknown.…”

Ca2+-activated Cl− channels at a glance

“…Similar to ANO1, ANO7 has a role in cancer, specifically prostate cancer (Bera et al, 2004). A set of dominant mutations in ANO5 was linked to the rare skeletal condition gnathodiaphyseal dysplasia (Tsutsumi et al, 2004), whereas recessive mutations were found to lead to limbgirdle muscular dystrophy or Miyoshi myopathy (Bolduc et al, 2010).…”

Role of anoctamins in cancer and apoptosis

“…Although a deeper understanding is currently lacking, it should be mentioned that various anoctamins have a role during murine embryogenesis [9]. Ano7 (TMEM17G, NGEP) has been detected in prostate cancer [52,53]. Studies indicate that the long version of NGEP is present on the plasma membrane of overexpressing LNCaP cells and is highly concentrated at cell-cell contact regions [53].…”