NFκB Signaling Directs Neuronal Fate Decision

Zhang, Yonggang; Hu, Wenhui

doi:10.5772/53503

Cited by 2 publications

(1 citation statement)

References 113 publications

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“…Upregulation of NF-κB and downregulation of pro-inflammatory cytokines points towards NF-κB activation with non-inflammatory function, such as angiogenesis which corresponds with the observed upregulation of VEGF-A and IL-6 markers. Another potential function of activated NF-κB together with Notch1 and STAT3 upregulation is control of astrogliosis and radial glia recruitment to the site of injury [27,28,[37][38][39][40]. The presence of Nestin positive cells at the site of injury supports the molecular data ( Fig.…”

Section: Mp Transplants Survive Due To a Decrease In Inflammation Andsupporting

confidence: 68%

Engineering advanced neural tissue constructs to mitigate acute cerebral inflammation after brain transplantation in rats

et al. 2019

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Stroke, traumatic brain injuries, and other similar conditions often lead to significant loss of functional brain tissue and associated disruption of neuronal signaling. A common strategy for replacing lost neurons is the injection of dissociated neural stem cells or differentiated neurons. However, this method is unlikely to be suitable for replacing large brain cavities, and the resulting distribution of neurons may lack the necessary architecture to support appropriate brain function. Engineered neural tissues may be a viable alternative. Cell death is a prominent concern in neuronal grafting studies, a problem that could be magnified with the transplantation of engineered neural tissues. Here, we examined the effect of one contributor to cell death, acute cerebral inflammation, on neuronal survival after the transplantation of bioengineered constructs based on silk scaffolds. We found evidence of a high degree of inflammation and poor neuronal survival after introducing engineered constructs into the motor cortex of rats. Integrating a corticosteroid (methylprednisolone) into the constructs resulted in significantly improved neuron survival during the acute phase of inflammation. The improved construct survival was associated with decreased markers of inflammation and an anti-inflammatory state of the immune system due to the steroid treatment.

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Section: Mp Transplants Survive Due To a Decrease In Inflammation Andsupporting

confidence: 68%

Engineering advanced neural tissue constructs to mitigate acute cerebral inflammation after brain transplantation in rats

et al. 2019

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Identification of undescribed Relb expression domains in the murine brain by new Relb:cre‐katushka reporter mice

Engelmann

Robinson‐Rechavi

Carlstedt

et al. 2020

Developmental Dynamics

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BackgroundNoncanonical NF‐κB signaling through activation of the transcription factor RelB acts as key regulator of cell lineage determination and differentiation in various tissues including the immune system. To elucidate temporospatial aspects of Relb expression, we generated a BAC transgenic knock‐in mouse expressing the fluorescent protein Katushka and the enzyme Cre recombinase under control of the murine Relb promoter (RelbCre‐Kat mice).ResultsCo‐expression of Katushka and Relb in fibroblast cultures and tissues of transgenic mice revealed highly specific reporter functions of the transgene. Crossing RelbCre‐Kat mice with ROSA26R reporter mice that allow for Cre‐mediated consecutive β‐galactosidase or YFP synthesis identified various Relb expression domains in perinatal and mature mice. Besides thymus and spleen, highly specific expression patterns were found in different neuronal domains, as well as in other nonimmune organs including skin, skeletal structures and kidney. De novo Relb expression in the mature brain was confirmed in conditional knockout mice with neuro‐ectodermal Relb deletion.ConclusionOur results demonstrate the usability of RelbCre‐Kat reporter mice for the detection of de novo and temporarily restricted Relb expression including cell and lineage tracing of Relb expressing cells. Relb expression during mouse embryogenesis and at adulthood suggests, beyond immunity, important functions of this transcription factor in neurodevelopment and CNS function.

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NFκB Signaling Directs Neuronal Fate Decision

Cited by 2 publications

References 113 publications

Engineering advanced neural tissue constructs to mitigate acute cerebral inflammation after brain transplantation in rats

Engineering advanced neural tissue constructs to mitigate acute cerebral inflammation after brain transplantation in rats

Identification of undescribed Relb expression domains in the murine brain by new Relb:cre‐katushka reporter mice

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