NF-κB-activated SPRY4-IT1 promotes cancer cell metastasis by downregulating TCEB1 mRNA via Staufen1-mediated mRNA decay

Zhao, Lin; Jiang, Longyang; Zhang, Ming; Qiang, Zhang; Guan, Qiutong; Li, Yalun; He, Miao; Zhang, Jingdong

doi:10.1038/s41388-021-01900-8

Cited by 18 publications

(10 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Multiple transcription factors (TFs) are activated during tumorigenesis and tumor progression. The TFs, such as STAT1, STAT3, ZEB1, NF-kB, have been found to mediate CRC progression via modulating the expression of lncRNAs (40)(41)(42)(43). YY1 is a multifunctional protein that can activate or inhibit gene expression according to changes in conditions (44).…”

Section: Discussionmentioning

confidence: 99%

A Positive Feedback Loop of lncRNA MIR31HG-miR-361-3p -YY1 Accelerates Colorectal Cancer Progression Through Modulating Proliferation, Angiogenesis, and Glycolysis

et al. 2021

View full text Add to dashboard Cite

BackgroundColorectal cancer (CRC) is a common malignant tumor with high metastatic and recurrent rates. This study probes the effect and mechanism of long non-coding RNA MIR31HG on the progression of CRC cells.Materials and MethodsQuantitative real-time PCR (qRT-PCR) was used to analyze the expression of MIR31HG and miR-361-3p in CRC tissues and normal tissues. Gain- or loss-of-function assays were conducted to examine the roles of MIR31HG, miR-361-3p and YY1 transcription factor (YY1) in the CRC progression. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and colony formation experiment were conducted to test CRC cell proliferation. CRC cell invasion was determined by Transwell assay. The glucose detection kit and lactic acid detection kit were utilized to monitor the levels of glucose and lactate in CRC cells. The glycolysis level in CRC cells was examined by the glycolytic stress experiment. Western blot was performed to compare the expression of glycolysis-related proteins (PKM2, GLUT1 and HK2) and angiogenesis-related proteins (including VEGFA, ANGPT1, HIF1A and TIMP1) in HUVECs. The binding relationships between MIR31HG and miR-361-3p, miR-361-3p and YY1 were evaluated by the dual-luciferase reporter assay and RNA immunoprecipitation (RIP).ResultsMIR31HG was up-regulated in CRC tissues and was associated with poorer prognosis of CRC patients. The in-vitro and in-vivo experiments confirmed that overexpressing MIR31HG heightened the proliferation, growth, invasion, glycolysis and lung metastasis of CRC cells as well as the angiogenesis of HUVECs. In addition, MIR3HG overexpression promoted YY1 mRNA and protein level, and forced overexpression of YY1 enhanced MIR31HG level. Overexpressing YY1 reversed the tumor-suppressive effect mediated by MIR31HG knockdown. miR-361-3p, which was inhibited by MIR31HG overexpression, repressed the malignant behaviors of CRC cells. miR-361-3p-mediated anti-tumor effects were mostly reversed by upregulating MIR31HG. Further mechanism studies illustrated that miR-361-3p targeted and negatively regulated the expression of YY1.ConclusionThis study reveals that MIR31HG functions as an oncogenic gene in CRC via forming a positive feedback loop of MIR31HG-miR-361-3p-YY1.

show abstract

Section: Discussionmentioning

confidence: 99%

A Positive Feedback Loop of lncRNA MIR31HG-miR-361-3p -YY1 Accelerates Colorectal Cancer Progression Through Modulating Proliferation, Angiogenesis, and Glycolysis

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Conversely, EMT can be triggered by SPRY4-IT1 in cervical cancer, colorectal cancer, and hepatocellular carcinomas [190][191][192]. What is more, SPRY4-IT1 is transcriptionally activated by NF-κB and promotes staufen-mediated degradation of transcription elongation factor B subunit 1 (TCEB1) mRNA to upregulate HIF-1α levels [193]. Thus, it is probable that SPRY4-IT1 can control the expression of other ncRNAs, such as circ_ZNF91, which is regulated by HIF-1α (see Section 2.5.3 about circ_ZNF91).…”

Section: Spry4-it1mentioning

confidence: 99%

Oncogenic Role of Exosomal Circular and Long Noncoding RNAs in Gastrointestinal Cancers

Yun

Choi

Son

et al. 2022

IJMS

View full text Add to dashboard Cite

Circular RNAs (circRNAs) and long noncoding RNAs (lncRNAs) are differentially expressed in gastrointestinal cancers. These noncoding RNAs (ncRNAs) regulate a variety of cellular activities by physically interacting with microRNAs and proteins and altering their activity. It has also been suggested that exosomes encapsulate circRNAs and lncRNAs in cancer cells. Exosomes are then discharged into the extracellular environment, where they are taken up by other cells. As a result, exosomal ncRNA cargo is critical for cell–cell communication within the cancer microenvironment. Exosomal ncRNAs can regulate a range of events, such as angiogenesis, metastasis, immune evasion, drug resistance, and epithelial-to-mesenchymal transition. To set the groundwork for developing novel therapeutic strategies against gastrointestinal malignancies, a thorough understanding of circRNAs and lncRNAs is required. In this review, we discuss the function and intrinsic features of oncogenic circRNAs and lncRNAs that are enriched within exosomes.

show abstract

“…It participates in the transport of transcripts to various subcellular localizations. Expression of SPRY4-IT1 is also activated by NF-κB/p65 ( 5 ).…”

Section: Cell Line Studiesmentioning

confidence: 99%

“…Using a panel of colon, breast, and ovarian cancer tissues, Zhao et al. have found that elevation of SPRY4-IT1 expression is associated with aggressive behavior and poor clinical outcome of patients ( 5 ). Another study has shown that SPRY4-IT1 overexpression in breast cancer tissues is associated with a larger neoplasm bulk and higher pathological stage ( 7 ).…”

Section: Human Studiesmentioning

confidence: 99%

A Review on the Role of SPRY4-IT1 in the Carcinogenesis

et al. 2022

View full text Add to dashboard Cite

Sprouty RTK signaling antagonist 4-intronic transcript 1 (SPRY4-IT1) is a long non-coding RNA (lncRNA) encoded by a gene located on 5q31.3. This lncRNA has a possible role in the regulation of cell growth, proliferation, and apoptosis. Moreover, since SPRY4-IT1 controls levels of lipin 2, it is also involved in the biosynthesis of lipids. During the process of biogenesis, SPRY4-IT1 is produced as a primary transcript which is then cleaved to generate a mature transcript which is localized in the cytoplasm. SPRY4-IT1 has oncogenic roles in diverse tissues. A possible route of participation of SPRY4-IT1 in the carcinogenesis is through sequestering miRNAs such as miR-101-3p, miR‐6882‐3p and miR-22-3p. The sponging effect of SPRY4-IT1 on miR-101 has been verified in colorectal cancer, osteosarcoma, cervical cancer, bladder cancer, gastric cancer and cholangiocarcinoma. SPRY4-IT1 has functional interactions with HIF-1α, NF-κB/p65, AMPK, ZEB1, MAPK and PI3K/Akt signaling. We explain the role of SPRY4-IT1 in the carcinogenesis according to evidence obtained from cell lines, xenograft models and clinical studies.

show abstract

NF-κB-activated SPRY4-IT1 promotes cancer cell metastasis by downregulating TCEB1 mRNA via Staufen1-mediated mRNA decay

Cited by 18 publications

References 46 publications

A Positive Feedback Loop of lncRNA MIR31HG-miR-361-3p -YY1 Accelerates Colorectal Cancer Progression Through Modulating Proliferation, Angiogenesis, and Glycolysis

A Positive Feedback Loop of lncRNA MIR31HG-miR-361-3p -YY1 Accelerates Colorectal Cancer Progression Through Modulating Proliferation, Angiogenesis, and Glycolysis

Oncogenic Role of Exosomal Circular and Long Noncoding RNAs in Gastrointestinal Cancers

A Review on the Role of SPRY4-IT1 in the Carcinogenesis

Contact Info

Product

Resources

About