Next-Generation Sequencing Reveals the Progression of COVID-19

In winter of 2020, SARS-CoV-2 emerged as a global threat, impacting not only health but also financial and political stability. To address the societal need for monitoring the spread of SARS-CoV-2, many existing diagnostic technologies were quickly adapted to detect SARS-CoV-2 RNA and antigens as well as the immune response, and new testing strategies were developed to accelerate time-to-decision. In parallel, the infusion of research support accelerated the development of new spectroscopic methods. While these methods have significantly reduced the impact of SARS-CoV-2 on society when coupled with behavioral changes, they also lay the groundwork for a new generation of platform technologies. With several epidemics on the horizon, such as the rise of antibiotic-resistant bacteria, the ability to quickly pivot the target pathogen of this diagnostic toolset will continue to have an impact.

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“…Next Generation Sequencing (NGS) testing allows surveillance, detection, and characterization of different SARS-CoV-2 strains. 50 …”

Section: Methods Making An Impactmentioning

confidence: 99%

COVID-19 Diagnostics: Past, Present, and Future

et al. 2021

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“…110,118 Furthermore, NGS technology was applied to investigate mechanisms of SARS-CoV-2 infection. 116 For example, RNA sequencing has been used to determine susceptible organs with higher expression of angiotensin-converting enzyme 2 (ACE2) receptor, which serves as a receptor for SARS-CoV-2. 119 NGS and single-cell RNA sequencing were used to determine expression of ACE2 receptor in numerous organs and cells after infection with SARS-CoV-2 116 , which will most definitely benefit diagnostic and therapeutic target identification.…”

Section: Sequencing During the Covid-19 Pandemicsmentioning

confidence: 99%

Sequencing of Nucleic Acids: from the First Human Genome to Next Generation Sequencing in COVID-19 Pandemic

Furlani

Kouter

Rozman

et al. 2021

ACSi

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Despite being around for more than 40 years, DNA sequencing is regarded as young technology in clinical medicine. As sequencing is becoming cheaper, faster and more accurate, it is rapidly being incorporated into clinical laboratories. In 2003, the completion of the first human genome opened the door to personalized medicine. Ever since it has been expected for genomics to widely impact clinical care and public health. However, many years can pass for genomic discoveries to reflect back and benefit the patients. DNA sequencing represents a less biased approach to diagnostics. It is not only a diagnostic tool, but can also influence clinical management and therapy. As new technologies rapidly emerge it is important for researchers and health professionals to have basic knowledge about the capabilities and drawbacks of the existing sequencing methods, and their use in clinical setting and research. This review provides an overview of nucleic acid sequencing technologies from historical perspective and later focuses on clinical utilization of sequencing. Some of the most promising areas are presented with selected examples from Slovenian researchers.

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“…Đối với công tác quản lý dịch bệnh ở người, cây trồng, vật nuôi, dữ liệu NGS góp phần phát hiện mầm bệnh, đặc biệt là các bệnh do vi sinh vật gây ra, phương thức lây truyền của tác nhân, nguy cơ bùng phát, qua đó kiểm soát sự xuất hiện và xác định cơ chế, nguồn lây lan của bệnh cũng như phát triển các phương pháp điều trị (Van Borm et al, 2014;Lefterova et al, 2015;Hadidi et al, 2016;Berry et al, 2020;Chen et al, 2021;Shahid et al, 2021). Coronavirus mới SARS-CoV-2 (gây bệnh Covid-19) đã gây ra đại dịch trên toàn cầu với khả năng lây lan rất cao.…”

Section: Trong Công Tác Quản Lý Dịch Bệnhunclassified

“…Do sự phát triển rất nhanh của dịch bệnh, việc xác định trình tự gen thông qua NGS và khai thác dữ liệu hệ gen đóng vai trò quan trọng ở nhiều khía cạnh, góp phần cung cấp thông tin về nguồn gốc và cơ chế lây nhiễm của SARS-CoV-2 ở người. Các công nghệ giải trình tự metagenome và giải trình tự tế bào đơn cũng được áp dụng để nghiên cứu các rối loạn về vi sinh vật đường ruột và di truyền miễn dịch của bệnh nhân COVID-19 (Chen et al, 2021). Việc áp dụng các kỹ thuật giải trình tự này có thể có ý nghĩa trong việc tìm kiếm các vật chủ SARS-CoV-2 trung gian mới nhằm ngăn chặn sự lây truyền giữa các loài.…”

Section: Trong Công Tác Quản Lý Dịch Bệnhunclassified

Genomics and big data: Research, development and applications

Hiền¹,

Văn²,

Oanh³

et al. 2021

Vietnam J. Biotechnol.

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Recent years, genomics and big data analytics have been widely applied and have significant impacts in various important areas of social life worldwide. The development of the next-generation sequencing (NGS) technologies, such as whole-genome sequencing (WGS), whole-exome sequencing (WES), transcriptome, and/or targeted sequencing, has enabled quickly generating the genomes of interested living organisms. Around the world many nations have invested in and promoted the development of genomics and big data analytics. A number of well-established projects on sequencing of human, animal, plant, and microorganism genomes to generate vast amounts of genomic data have been conducted independently or as collaborative efforts by national or international research networks of scientists specializing in different technical fields of genomics, bioinformatics, computational and statistical biology, automation, artificial intelligence, etc. Complicated and large genomic datasets have been effectively established, storage, managed, and used. Vietnam supports this new field of study through setting up governmental authorized institutions and conducting genomic research projects of human and other endemic organisms. In this paper, the research, development, and applications of genomic big data are reviewed with focusing on: (i) Available sequencing technologies for generating genomic datasets; (ii) Genomics and big data initiatives worldwide; (iii) Genomics and big data analytics in selected countries and Vietnam; (iv) Genomic data applications in key areas including medicine for human health care, agriculture - forestry, food safety, and environment.

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Next-Generation Sequencing Reveals the Progression of COVID-19

Cited by 47 publications

References 134 publications

COVID-19 Diagnostics: Past, Present, and Future

COVID-19 Diagnostics: Past, Present, and Future

Sequencing of Nucleic Acids: from the First Human Genome to Next Generation Sequencing in COVID-19 Pandemic

Genomics and big data: Research, development and applications

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