Next-generation sequencing of the basal cell carcinoma miRNome and a description of novel microRNA candidates under neoadjuvant vismodegib therapy: an integrative molecular and surgical case study

Sand, Michael; Bechara, Falk G.; Gambichler, Thilo; Sand, Daniel; Friedländer, Marc R.; Bromba, Michael; Schnabel, Rolf; Hessam, Schapoor

doi:10.1093/annonc/mdv551

Cited by 24 publications

(25 citation statements)

References 42 publications

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“…miR-197-3p belongs to miR-197 family and has been demonstrated to have a low expression in primary biliary cirrhosis [12]. In basal cell carcinoma, miR-197-3p is usually overexpressed, and can inhibit keratinocyte proliferation and migration [13]. LINC00312 has been reported to have a negative correlation with miR-197-3p in bladder cancer.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of long intergenic noncoding RNA LINC00312 inhibits the invasion and migration of thyroid cancer cells by down-regulating microRNA-197-3p

Huang

Yan

et al. 2017

Bioscience Reports

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The study evaluated the ability of long intergenic noncoding RNA LINC00312 (LINC00312) to influence the proliferation, invasion, and migration of thyroid cancer (TC) cells by regulating miRNA-197-3p. TC tissues and adjacent normal tissues were collected from 211 TC patients. K1 (papillary TC), SW579 (squamous TC), and 8505C (anaplastic TC) cell lines were assigned into a blank, negative control (NC), LINC00312 overexpression, miR-197-3p inhibitors, and LINC00312 overexpression + miR-197-3p mimics group. The expression of LINC00312, miR-197-3p, and p120 were measured using quantitative real-time PCR (qRT-PCR) and Western blotting. Cell proliferation was assessed via CCK8 assay, cell invasion through the scratch test, and cell migration via Transwell assay. In comparison with adjacent normal tissues, the expression of LINC00312 is down-regulated and the expression of miR-197-3p is up-regulated in TC tissues. The dual luciferase reporter gene assay confirmed that P120 is a target of miR-197-3p. The expression of LINC00312 and p120 was higher in the LINC00312 overexpression group than in the blank and NV groups. However, the expression of miR-197-3p was lower in the LINC00312 overexpression group than in the blank and NC groups. The miR-197-3p inhibitors group had a higher expression of miR-197-3p, but a lower expression of p120 than the blank and NC groups. The LINC00312 overexpression and miR-197-3p inhibitor groups had reduced cell proliferation, invasion and migration than the blank and NC groups. These results indicate that a LINC00312 overexpression inhibits the proliferation, invasion, and migration of TC cells and that this can be achieved by down-regulating miR-197-3p.

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Section: Introductionmentioning

confidence: 99%

Overexpression of long intergenic noncoding RNA LINC00312 inhibits the invasion and migration of thyroid cancer cells by down-regulating microRNA-197-3p

Huang

Yan

et al. 2017

Bioscience Reports

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“…Therapeutic cocktails that combine the various treatment modalities and target multiple pathways associated with BCCs may offer the unique opportunity for treating metastatic and advanced BCCs. Additionally, the finding that differentially expressed miRNAs was found in vismodegib treated BCC tissue warrants additional investigations to confirm the role noncoding RNA in promoting tumor resistance . Future studies can explore strategies to modulate miRNAs that are associated with BCC pathogenesis and resistance.…”

Section: Summary and Future Prospectsmentioning

confidence: 97%

“…Additionally, the finding that differentially expressed miRNAs was found in vismodegib treated BCC tissue warrants additional investigations to confirm the role noncoding RNA in promoting tumor resistance. 202 Future studies can explore strategies to modulate miRNAs that are associated with BCC pathogenesis and resistance. Finally, the discovery of mutations beyond HH could be important to develop therapies that target the novel additional driver signaling pathways that could be central to BCC growth progression despite targeting HH.…”

Section: Combination Therapiesmentioning

confidence: 99%

Basal cell carcinoma pathogenesis and therapy involving hedgehog signaling and beyond

Bakshi

Chaudhary

Rana

et al. 2017

Molecular Carcinogenesis

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Basal cell carcinoma (BCC) of the skin is driven by aberrant hedgehog signaling. Thus blocking this signaling pathway by small molecules such as vismodegib inhibits tumor growth. Primary cilium in the epidermal cells plays an integral role in the processing of hedgehog signaling-related proteins. Recent genomic studies point to the involvement of additional genetic mutations that might be associated with the development of BCCs, suggesting significance of other signaling pathways, such as WNT, NOTCH, mTOR, and Hippo, aside from hedgehog in the pathogenesis of this human neoplasm. Some of these pathways could be regulated by noncoding microRNA. Altered microRNA expression profile is recognized with the progression of these lesions. Stopping treatment with Smoothened (SMO) inhibitors often leads to tumor reoccurrence in the patients with basal cell nevus syndrome, who develop 10–100 of BCCs. In addition, the initial effectiveness of these SMO inhibitors is impaired due to the onset of mutations in the drug-binding domain of SMO. These data point to a need to develop strategies to overcome tumor recurrence and resistance and to enhance efficacy by developing novel single agent-based or multiple agents-based combinatorial approaches. Immunotherapy and photodynamic therapy could be additional successful approaches particularly if developed in combination with chemotherapy for inoperable and metastatic BCCs.

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“…Project such as the Encyclopedia of DNA Elements project (ENCODE), which aims to map all functional elements in the human genome, has made great progress . Methods of RNA microarray and novel high‐throughput technologies such as next‐generation sequencing (NGS) provide powerful tools for the analysis of miRNA transcription profiles and their target genes in diseases . miRNAs regulate target genes through the base pair interaction of seed region (nucleotide 2‐7 of the miRNA) with target mRNA 3′UTR.…”

Section: Perspective Of Mirna Therapiesmentioning

confidence: 99%

“…150 Methods of RNA microarray and novel high-throughput technologies such as next-generation sequencing (NGS) provide powerful tools for the analysis of miRNA transcription profiles and their target genes in diseases. [151][152][153][154] miRNAs regulate target genes through the base pair interaction of seed region (nucleotide 2-7 of the miRNA) with target mRNA 3′UTR. A sequence of this length will occur with much high frequency in the whole genome; therefore, the prediction of functional miRNA target sites is challenging but will be critical task.…”

Section: Per S Pec Tive Of Mirna Ther Apie Smentioning

confidence: 99%

Dysregulation of miRNA and its potential therapeutic application in schizophrenia

Cao

Zhen

2018

CNS Neurosci Ther

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Although it is generally believed that genetic and developmental factors play critical roles in pathogenesis of schizophrenia, however, the precise etiological mechanism of schizophrenia remains largely unknown. Over past decades, miRNAs have emerged as an essential post-transcriptional regulator in gene expression regulation. The importance of miRNA in brain development and neuroplasticity has been well-established. Abnormal expression and dysfunction of miRNAs are known to involve in the pathophysiology of many neuropsychiatric diseases including schizophrenia. In this review, we summarized the recent findings in the schizophrenia-associated dysregulation of miRNA and functional roles in the development and pathogenesis of schizophrenia. We also discussed the potential therapeutic implications of miRNA regulation in the illness.

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Next-generation sequencing of the basal cell carcinoma miRNome and a description of novel microRNA candidates under neoadjuvant vismodegib therapy: an integrative molecular and surgical case study

Cited by 24 publications

References 42 publications

Overexpression of long intergenic noncoding RNA LINC00312 inhibits the invasion and migration of thyroid cancer cells by down-regulating microRNA-197-3p

Overexpression of long intergenic noncoding RNA LINC00312 inhibits the invasion and migration of thyroid cancer cells by down-regulating microRNA-197-3p

Basal cell carcinoma pathogenesis and therapy involving hedgehog signaling and beyond

Dysregulation of miRNA and its potential therapeutic application in schizophrenia

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