Next-generation sequencing, isolation and characterization of 14 microsatellite loci of Canthon cyanellus (Coleoptera: Scarabaeidae)

Abstract

BackgroundWe used Illumina paired-end-sequencing to isolate and characterize microsatellites of Canthon cyanellus, a Neotropical roller dung beetle encompassing several lineages within its distribution range. Methods and ResultsWe examined C. cyanellus specimens collected at eight different localities in Mexico (two or three specimens per locality). We initially made ampli cation tests with 16 loci, but two of these showed ampli cation problems; thus, we evaluated 14 loci. The 14 microsatellites were polymorphic, with 2-16 alleles each. The expected and observed heterozygosity ranged from 0.11 to 0.76 and from 0.20 to 0.78, respectively. ConclusionsThese microsatellites will help to assess structure at the population and lineage levels, identify zones of potential hybridization between lineages, and draw a more precise geographic delimitation of Canthon cyanellus lineages.

Evolutionary history and diversity in the ball roller beetle Canthon cyanellus

“…Currently, 14 polymorphic microsatellites have been developed (2-16 alleles each) for the populations of C. cyanellus that belong to the different clades identified by Nolasco-Soto et al (2017, 2020. These microsatellites contain private alleles that suggest their potential use to assess the genetic structure and more precise geographic delimitation of clades within C. cyanellus (Arce-Valdés et al, 2021).…”

“…Canthon cyanellus LeConte, 1859 is a necrophagous roller beetle from the Neotropics (Favila, 2001), which provides valuable ecosystem services similar to other Scarabaeinae (e.g., nutrient recycling, fly control, carrion removal, secondary seed dispersal, soil aeration; Nichols et al, 2008). It is a well-studied species in ecological and evolutionary aspects related to reproductive behavior (Favila, 2001;Favila et al, 2005;Chamorro-Florescano and Favila, 2008, 2009Chamorro-Florescano et al, 2011, 2017, larval development (Hernández-Martínez and Martínez, 2003;Martínez, 2005), chemical communication (Bellés and Favila, 1983;Ortiz-Domínguez et al, 2006a,b;Cortez et al, 2012Cortez et al, , 2015Favila et al, 2012), population genetics and phylogeography (Ortiz-Domínguez et al, 2010;Nolasco-Soto et al, 2017;Arce-Valdés et al, 2021), among others. Traditionally, the taxonomy of C. cyanellus was based in non-informative morphological characters like variations in body color (Halffter, 1961).…”

Assessment of Diversity and Genetic Variability of Guava (<em>Psidium guajava</em>) by Means of Microsatellite Markers in Montería, Córdoba-Colombia

“…Molecular tools are used for this purpose, with microsatellite markers being prominent among them. These markers are repetitive DNA sequences of 1 to 6 base pairs and are widely used to understand how living organisms change over time [7].…”