Next-generation sequencing identification of pathogenic bacterial genes and their relationship with fecal indicator bacteria in different water sources in the Kathmandu Valley, Nepal

Shrestha, Rajani Ghaju; Tanaka, Yasuhiro; Malla, Bikash; Bhandari, Dinesh; Tandukar, Sarmila; Inoue, Daisuke; Sei, Kazunari; Sherchand, Jeevan Bahadur; Haramoto, Eiji

doi:10.1016/j.scitotenv.2017.05.105

Cited by 48 publications

(64 citation statements)

References 28 publications

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“…A total of 28 water samples including 10 from commercial jar water, 14 from community well groundwater, and 4 from environmental water, all of which were collected from the Kathmandu Valley from August to September 2014 5), 10) , were used in this study. Ten jar water samples investigated here were selected from 30 different brands of jar water investigated previously 5) based on the detection of Escherichia coli and high and low concentrations of total coliforms.…”

Section: Water Samplesmentioning

confidence: 99%

“…Environmental water samples included 2 spring water and 2 river water samples. Community well groundwater and environmental water samples investigated here were also selected from 37 samples investigated previously 10) in the light of the occurrence and abundance of E. coli and total coliforms and geographical locations.…”

Section: Water Samplesmentioning

confidence: 99%

“…Previous studies have demonstrated the presence of fecal indicators and selected pathogens, including protozoa, viruses, and pathogenic bacteria, in jar water 4), 5) and groundwater in the valley 6)- 9) , confirming that the water sources were unsafe. Additionally, very recently, we investigated total bacterial communities in groundwater and spring and river water samples collected from the valley using nextgeneration sequencing (NGS), and revealed the occurrence of diverse potential pathogenic bacteria, relatively abundant occurrence of several potential pathogenic bacteria such as Acinetobacter, Arcobacter and Clostridium, and no sufficient correlation between fecal indicator bacteria and potential pathogenic bacteria 10) . The evidence emphasized the importance of further investigation of the occurrence of pathogenic bacteria in water sources in the valley.…”

Section: Introductionmentioning

confidence: 99%

“…DNA microarray analysis targeting > 900 different pathogenic bacterial species/ groups 9) was performed, and genus-specific PCR was used to confirm the occurrence and quantify selected pathogenic bacteria. DNA microarray analysis which targets only pathogenic bacteria would be useful especially for clarifying the occurrence of low abundance pathogenic bacteria that might be overlooked in NGS targeting whole bacterial communities that was conducted in our previous study 10) .…”

Section: Introductionmentioning

confidence: 99%

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Comprehensive Detection of Pathogenic Bacteria in Jar Water, Community Well Groundwater, and Environmental Water in the Kathmandu Valley, Nepal

大介

隼人

Malla

et al. 2018

Japanese J. Wat. Treat. Biol.

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Microbial contamination of water sources remains a serious problem in the Kathmandu Valley, Nepal. This study was conducted to comprehensively investigate the occurrence of pathogenic bacteria in water samples collected from the valley, most of which were used for drinking and other domestic purposes. The water samples consisted of 10 samples of commercial jar water, 10 of groundwater from shallow dug wells, 3 of groundwater from deep tube wells, 1 of stone spout water, 2 of spring water, and 2 of river water. DNA microarray analysis targeting 941 different pathogenic bacterial species/groups showed the prevalence of Acinetobacter, Arthrobacter, Brevibacterium, Pseudomonas, and Legionella and the presence of biosafety level 3 pathogens in some samples, which were partially confirmed by genus-specific PCR quantification for Legionella and Brucella. Additionally, the occurrence of most pathogens detected via DNA microarray analysis was not correlated with that of fecal indicator bacteria (total coliforms and Escherichia coli). These results will aid in understanding the overall picture of pathogenic bacterial contamination and in screening important pathogens that should be investigated in detail.

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Section: Water Samplesmentioning

confidence: 99%

Section: Water Samplesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comprehensive Detection of Pathogenic Bacteria in Jar Water, Community Well Groundwater, and Environmental Water in the Kathmandu Valley, Nepal

大介

隼人

Malla

et al. 2018

Japanese J. Wat. Treat. Biol.

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“…Therefore, it was chosen as the biological indicator of water quality (Edberg 2000). The occurrence of coliform in potable water indicates the presence of other potential pathogenic bacteria in water (Cappuccino and Sherman, 2002;Zareen 2014;Shrestha 2017). To determine the treatment efficacy of water treatment plants, total coliforms are one of the best indicators (Tallon 2005).…”

Section: Introductionmentioning

confidence: 99%

Risk assessment and biofilm formation of bacterial communities associated with drinking water distribution system

Ishaq¹,

Ali²

2018

JEB

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Aim : Methodology :Results : Interpretation :Potable water can be contaminated with variety of bacterial pathogens during the distribution process. The aim of this study was to analyze the bacterial risks associated with drinking water supply system of the city Kasur, Pakistan.Water analysis was accomplished by following the most probable number (MPN) method. Bacterial diversity was determined by 16S rRNA gene sequencing. Screening for O157 antigen was conducted for all the identified strains of . Strains were also screened for antibiotic susceptibility and biofilm formation.Analysis showed 2400 MPN index for few water samples. 16S rRNA sequencing confirmed the presence of bacterial strains belonging to the genera of and Serological analysis also detected the presence of pathogenic strains of O157. Antibiotic sensitivity pattern showed the resistance of strains against nitrofurantoin, cephalexin, nalidixic acid and ampicillin. For biofilm formation, DG1 and J4 were the most active biofilm producer in single cultures.Majority of the water samples were contaminated in terms of coliforms. Biofilm formation by bacterial strains indicated that water distribution network might be colonized by potential human pathogens.

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Detection and occurrence of indicator organisms and pathogens

Motlagh

Yang

2019

Water Environment Research

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This review paper focuses on detection and quantification techniques of indicator organisms that can be used for water quality assessment. The environmental pathogens that are critical to understand and better evaluate water quality are also discussed in this paper. Several recent studies using culture-independent methods such as microbial source tracking, pulsed field gel electrophoresis, mitochondrial DNA, and next generation sequencing to assess various environmental samples and water bodies have been reviewed.

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Next-generation sequencing identification of pathogenic bacterial genes and their relationship with fecal indicator bacteria in different water sources in the Kathmandu Valley, Nepal

Cited by 48 publications

References 28 publications

Comprehensive Detection of Pathogenic Bacteria in Jar Water, Community Well Groundwater, and Environmental Water in the Kathmandu Valley, Nepal

Comprehensive Detection of Pathogenic Bacteria in Jar Water, Community Well Groundwater, and Environmental Water in the Kathmandu Valley, Nepal

Risk assessment and biofilm formation of bacterial communities associated with drinking water distribution system

Detection and occurrence of indicator organisms and pathogens

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