Next generation sequencing, biochemical characterization, metabolic pathway analysis of novel probiotic Pediococcus acidilactici NCDC 252 and it’s evolutionary relationship with other lactic acid bacteria

Bansal, Poonam; Kumar, Raman; Singh, Jasbir; Dhanda, Suman

doi:10.1007/s11033-019-05022-z

Cited by 23 publications

(18 citation statements)

References 34 publications

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“…In that direction, more research about bacteria components of the intestinal microbiota that could become new candidates for next-generation probiotics with specific biotherapeutic and detoxifying role could be developed [ 30 ]. Moreover, this area of research is now evolving in parallel with omics methodologies [ 7 ], improved identification, culturing, and next-generation sequencing technologies [ 31 ].…”

Section: Introductionmentioning

confidence: 99%

Probiotic Strains and Intervention Total Doses for Modulating Obesity-Related Microbiota Dysbiosis: A Systematic Review and Meta-analysis

et al. 2020

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Obesity is a growing health threat worldwide. Administration of probiotics in obesity has also parallelly increased but without any protocolization. We conducted a systematic review exploring the administration pattern of probiotic strains and effective doses for obesity-related disorders according to their capacity of positively modulating key biomarkers and microbiota dysbiosis. Manuscripts targeting probiotic strains and doses administered for obesity-related disorders in clinical studies were sought. MEDLINE, Scopus, Web of Science, and Cochrane Library databases were searched using keywords during the last fifteen years up to April 2020. Two independent reviewers screened titles, abstracts, and then full-text papers against inclusion criteria according to PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines. From 549 interventional reports identified, we filtered 171 eligible studies, from which 24 full-text assays were used for calculating intervention total doses (ITD) of specific species and strains administered. Nine of these reports were excluded in the second-step because no specific data on gut microbiota modulation was found. Six clinical trials (CT) and 9 animal clinical studies were retained for analysis of complete outcome prioritized (body mass index (BMI), adiposity parameters, glucose, and plasma lipid biomarkers, and gut hormones). Lactobacillus spp. administered were double compared to Bifidobacterium spp.; Lactobacillus as single or multispecies formulations whereas most Bifidobacteria only through multispecies supplementations. Differential factors were estimated from obese populations’ vs. obesity-induced animals: ITD ratio of 2 × 106 CFU and patterns of administrations of 11.3 weeks to 5.5 weeks, respectively. Estimation of overall probiotics impact from selected CT was performed through a random-effects model to pool effect sizes. Comparisons showed a positive association between the probiotics group vs. placebo on the reduction of BMI, total cholesterol, leptin, and adiponectin. Moreover, negative estimation appeared for glucose (FPG) and CRP. While clinical trials including data for positive modulatory microbiota capacities suggested that high doses of common single and multispecies of Lactobacillus and Bifidobacterium ameliorated key obesity-related parameters, the major limitation was the high variability between studies and lack of standardized protocols. Efforts in solving this problem and searching for next-generation probiotics for obesity-related diseases would highly improve the rational use of probiotics.

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Section: Introductionmentioning

confidence: 99%

Probiotic Strains and Intervention Total Doses for Modulating Obesity-Related Microbiota Dysbiosis: A Systematic Review and Meta-analysis

et al. 2020

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“…Lactic acid bacteria such as L. plantarum (rod shaped) and P. acidilactici (coccoid shaped), are non-spore forming, facultative anaerobes that can ferment in the presence and absence of oxygen and tolerate a range of temperature and pH environments. P. acidilactici is homofermentative ( Bansal et al., 2019 ), whereas L. plantarum WCFS1 can switch between homofermentative and heterofermentative metabolism whereby ethanol, acetic acid and carbon dioxide are also produced ( Botta et al., 2017 ). The production of lactic acid results in the bacteria progressively acidifying their environment as they grow, thereby introducing a self-imposed stress on the microbiome.…”

Section: Resultsmentioning

confidence: 99%

The Application of Imaging Flow Cytometry for Characterisation and Quantification of Bacterial Phenotypes

Power

Barber

Groenhof

et al. 2021

Front. Cell. Infect. Microbiol.

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Bacteria modify their morphology in response to various factors including growth stage, nutrient availability, predation, motility and long-term survival strategies. Morphological changes may also be associated with specific physiological phenotypes such as the formation of dormant or persister cells in a “viable but non-culturable” (VBNC) state which frequently display different shapes and size compared to their active counterparts. Such dormancy phenotypes can display various degrees of tolerance to antibiotics and therefore a detailed understanding of these phenotypes is crucial for combatting chronic infections and associated diseases. Cell shape and size are therefore more than simple phenotypic characteristics; they are important physiological properties for understanding bacterial life-strategies and pathologies. However, quantitative studies on the changes to cell morphologies during bacterial growth, persister cell formation and the VBNC state are few and severely constrained by current limitations in the most used investigative techniques of flow cytometry (FC) and light or electron microscopy. In this study, we applied high-throughput Imaging Flow Cytometry (IFC) to characterise and quantify, at single-cell level and over time, the phenotypic heterogeneity and morphological changes in cultured populations of four bacterial species, Bacillus subtilis, Lactiplantibacillus plantarum, Pediococcus acidilactici and Escherichia coli. Morphologies in relation to growth stage and stress responses, cell integrity and metabolic activity were analysed. Additionally, we were able to identify and morphologically classify dormant cell phenotypes such as VBNC cells and monitor the resuscitation of persister cells in Escherichia coli following antibiotic treatment. We therefore demonstrate that IFC, with its high-throughput data collection and image capture capabilities, provides a platform by which a detailed understanding of changes in bacterial phenotypes and their physiological implications may be accurately monitored and quantified, leading to a better understanding of the role of phenotypic heterogeneity in the dynamic microbiome.

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Section: Discussionmentioning

confidence: 99%

“…Enterococci have reported to play a major role in ripening and aroma development in certain cheeses in southern Europe (Portugal, Spain, Italy and Greece) [44]. In Cebreiro, Kefalotyri, Manchego, Picante da Beira Baixa and Teleme cheeses, enterococci are the predominant microorganisms in the fully ripened cheese [44]. The beneficial role of enterococci in cheese-making has attributed to the lipolysis of milk by esterases [45] and enterococcal strains have been included in certain starter cultures.…”

Section: Discussionmentioning

confidence: 99%

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The microbiology of Kasseri cheese during the maturation

Bintsis¹

2021

World J. Adv. Res. Rev.

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The microbiology of Kasseri cheese, a Protected Designation of Origin (PDO) cheese of pasta-filata type was studied in order to identify the dominant species and strains that may contribute to the maturation process. Chemical composition and microbiological quality of Kasseri cheese samples from two different dairies during the maturation was studied at 0, 7, 25, 60 and 90 days of the maturation. Lactic acid bacteria and Enterococcus spp. were found to be the dominant microflora in fresh cheese. P. pentosaceous and P. acidilactici, E. hirae, E. faecium, E. durans and E. gallinarium, together with facultatively heterofermentative lactobacilli were found to be the dominant microflora. Since these strains are dominating the microflora of Kasseri cheese during maturation, the enzymic system need to be further studied in order to select the proper strains for adjunct culture in Kasseri cheese.

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Next generation sequencing, biochemical characterization, metabolic pathway analysis of novel probiotic Pediococcus acidilactici NCDC 252 and it’s evolutionary relationship with other lactic acid bacteria

Cited by 23 publications

References 34 publications

Probiotic Strains and Intervention Total Doses for Modulating Obesity-Related Microbiota Dysbiosis: A Systematic Review and Meta-analysis

Probiotic Strains and Intervention Total Doses for Modulating Obesity-Related Microbiota Dysbiosis: A Systematic Review and Meta-analysis

The Application of Imaging Flow Cytometry for Characterisation and Quantification of Bacterial Phenotypes

The microbiology of Kasseri cheese during the maturation

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