Next-generation sequencing-based microRNA profiling of mice testis subjected to transient heat stress

Rao, Meng; Zeng, Zhengyan; Li, Tang; Cheng, Guiping; Xia, Wei; Zhu, Changhong

doi:10.18632/oncotarget.22900

Cited by 20 publications

(17 citation statements)

References 37 publications

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“…For example, Guo et al (2017) reported that miR‐423 was a potential therapeutic target for the premature ovarian insufficiency leading to infertility. Rao et al (2017) reported that miR‐423 participated in the regulation of germ cell apoptosis, which became a potential candidate target in the process of male infertility. Low sperm motility is a frequent reason for fertilization failure of in vitro fertilization (IVF).…”

Section: Introductionmentioning

confidence: 99%

Upregulated microRNA‐423‐5p promotes oxidative stress through targeting glutathione S‐transferase mu 1 in asthenozoospermia

Zhang

Zuo

Cao

2021

Molecular Reproduction Devel

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The dysregulation of microRNAs (miRNAs) plays an important role in asthenozoospermia. This study evaluated the sperm microRNA‐423‐5p (miR‐423‐5p) expression in asthenozoospermia and normozoospermia, exploring the role of miR‐423‐5p in asthenozoospermia. Eighty participants were divided into asthenozoospermic (AZS, n = 40) and normozoospermic (Norm, n = 40) groups. Fresh semen samples were collected and the sperm cells were separated. Quantitative Real‐Time polymerase chain reaction was used to measure the sperm miR‐423‐5p level. Receiver operating characteristic curve (ROC) was employed to test the diagnostic performance of miR‐423‐5p in asthenospermia. Dual‐reporter luciferase assay was adopted to confirm the target gene of miR‐423‐5p. The target gene level in asthenozoospermia and normozoospermia was measured, and the biological function of target gene in asthenozoospermia was evaluated. Results showed that the miR‐423‐5p expression level in the AZS group was higher than that in Norm group, which was positively correlated with the severity of asthenozoospermia. ROC analysis of miR‐423‐5p showed an area under curve (AUC) of 0.69 (95% confidence interval = 0.57–0.80, p <0 .01), with 80% sensitivity and 60% specificity. Glutathione S‐transferase mu 1 (GSTM1) is a target gene of miR‐423‐5p, which significantly decreased in the AZS group. Compared with Norm group, glutathione S‐transferase (GST) activity and total antioxidant capacity (TAC) level decreased, while malondialdehyde (MDA) level increased in the AZS group. Furthermore, GST activity and TAC level were negatively correlated with miR‐423‐5p expression, while MDA level was positively correlated with miR‐423‐5p expression. In conclusion, the sperm miR‐423‐5p level significantly was upregulated in asthenozoospermia. High‐level miR‐423‐5p inhibited sperm motility through targeting GSTM1 to promote oxidative stress.

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Section: Introductionmentioning

confidence: 99%

Upregulated microRNA‐423‐5p promotes oxidative stress through targeting glutathione S‐transferase mu 1 in asthenozoospermia

Zhang

Zuo

Cao

2021

Molecular Reproduction Devel

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“…To date, roughly 500–1,000 miRNAs (both conserved and nonconserved) are found in the human genome that targeting >30% of human protein‐coding genes (Bentwich et al, 2005; Yang et al, 2017). Recent studies have revealed that specific miRNA contributes to the modulation of oxidative stress and their irregular results due to the impairment in spermatogenesis of infertile males (Kotaja, 2014; M. Rao et al, 2017). This is evident that miRNA‐122a plays an important role in germ cell development.…”

Section: Introductionmentioning

confidence: 99%

Oxidative stress‐related miRNAs in spermatozoa may reveal the severity of damage in grade III varicocele

et al. 2020

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Varicocele is characterised by abnormal dilation of the pampiniform plexus veins and stasis of the blood flow, and it is one of the leading causes of male infertility worldwide (Jensen et al., 2017). Although different factors, such as the reflux of renal and adrenal metabolites, hormonal upset, testicular hypoxia, elevated temperature in the scrotum and oxidative stress are involved in the pathophysiology of varicocele, the precise mechanisms of sperm abnormality following varicocele have not been fully understood (Hendin, 1999; Saleh et al., 2003). An important event in the pathophysiology of the varicocele is the high elevation of reactive oxygen species (ROS) (Cho,

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“…It is involved in the regulation of cell proliferation and apoptosis. In our previous study, we sequenced the miRNA of mouse testes after heat stress 9 and, for the first time, found that miR‐128‐3p expression is downregulated after heat stress. Previous research on miR‐128‐3p has mainly focused on tumor‐related diseases; few reports of miR‐128‐3p in relation to the reproductive system are available.…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies have found that microRNAs (miRNAs) play an important role in regulating spermatogenesis, especially spermatogenic meiotic division, apoptosis, and Sertoli cell function 6‐8 . In previous experiments, our team 9 identified some differentially expressed miRNAs (eg, miR‐449a‐3p, miR‐92a‐1‐5p, miR‐423‐3p, and miR‐128‐3p) after testicular heat stress, which are closely related to germ cell apoptosis and are involved in multiple pathways (eg, ribosomal, HIF‐1, and MAPK pathways). These pathways may also be critical in heat stress‐induced testicular injury.…”

Section: Introductionmentioning

confidence: 97%

“…Its expression is lower in the ovarian granulosa cells of young women with reduced ovarian reserve, 16 as well as in mice with re‐infection of genital chlamydia 17 . In our previous study, miR‐128‐3p was also found to be lower in the testes of mice subjected to heat stress 9 . Therefore, miR‐128‐3p was selected for further analysis in this study.…”

Section: Introductionmentioning

confidence: 97%

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The role of miR‐128‐3p through MAPK14 activation in the apoptosis of GC2 spermatocyte cell line following heat stress

Zou

Cheng

et al. 2020

Andrology

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Background MicroRNAs play a crucial role in the regulation of spermatogenesis. For example, miR‐128‐3p expression is known to decrease significantly after testicular hyperthermia, but the regulatory effect of this change on the spermatogenesis damage caused by heat stress remains unclear. Objectives This study aimed to verify whether the target gene of miR‐128‐3p is MAPK14, which affects spermatogenic cell proliferation and apoptosis under testicular hyperthermia. Materials and methods Mouse testis and GC2 spermatocyte cell line heat stress models were established. miR‐128‐3p expression before and after heat stress was analyzed by reverse transcription polymerase chain reaction. MAPK14 and p‐MAPK14 expression was detected by Western blot, and cell apoptosis was analyzed by Annexin V‐FITC/PI. Subsequently, miR‐128‐3p inhibitors and mimics were used to interfere with spermatocytes before and after heat stress, respectively, for correlation detection. Results Compared with the control group, the heat stress group showed decreased miR‐128‐3p expression, increased p‐MAPK14 expression, and decreased cell proliferation activity. In the GC2‐spd cell line in vitro, miR‐128‐3p inhibitors were found to upregulate p‐MAPK14 expression, reduce cell proliferation activity, and increase apoptosis, consistent with the results obtained in the heat treatment alone. Furthermore, miR‐128‐3p mimics transfected in the GC2 cells after heat stress reduced p‐MAPK14 expression, alleviated the decrease in cell proliferation, and decreased the apoptosis level. Conclusions The downregulation of miR‐128‐3p expression plays an important role in spermatogenesis damages after testicular hyperthermia, which is probably attributable to the activation of the MAPK signaling pathway. Downregulated miR‐128‐3p expression induces the apoptosis and inhibits the proliferation of spermatogenic cells by promoting MAPK14 phosphorylation.

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Next-generation sequencing-based microRNA profiling of mice testis subjected to transient heat stress

Cited by 20 publications

References 37 publications

Upregulated microRNA‐423‐5p promotes oxidative stress through targeting glutathione S‐transferase mu 1 in asthenozoospermia

Upregulated microRNA‐423‐5p promotes oxidative stress through targeting glutathione S‐transferase mu 1 in asthenozoospermia

Oxidative stress‐related miRNAs in spermatozoa may reveal the severity of damage in grade III varicocele

The role of miR‐128‐3p through MAPK14 activation in the apoptosis of GC2 spermatocyte cell line following heat stress

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