Next Generation Sequencing Analysis of miRNAs: MiR-127-3p Inhibits Glioblastoma Proliferation and Activates TGF-β Signaling by Targeting SKI

Jiang, Hongyi; Jin, Chengmeng; Liu, Jie; Hua, Dasong; Zhou, Fan; Lou, Xiaoyan; Zhao, Na; Lan, Qing; Huang, Qiang; Yoon, Jae-Geun; Zheng, Shu; Lin, Biaoyang

doi:10.1089/omi.2013.0122

Cited by 45 publications

(43 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study, we found that miR-127 is commonly downregulated in HCC tissues and hepatoma cell lines. Our data are in accordance with some previous studies that showed that miR-127 is downregulated in cancers, including primary prostate tumors, bladder tumors, colon tumors [18], primary breast tumors [15], gastric cancer [19], and glioblastoma [23]. However, in the serum of patients with esophageal squamous cell carcinoma, the concentration of miR-127 is significantly higher than that obtained in normal controls [24].…”

Section: Discussionsupporting

confidence: 92%

MicroRNA-127 Post-Transcriptionally Downregulates Sept7 and Suppresses Cell Growth in Hepatocellular Carcinoma Cells

Zhou

Yang

et al. 2014

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: Hepatocellular carcinoma is one of the most common cancers worldwide. It has been suggested that microRNAs, a class of small regulatory RNAs, are associated with tumorigenesis by targeting the mRNAs of hundreds of genes that modulate a variety of biological processes, including cellular differentiation, apoptosis, metabolism, and proliferation. Methods/Results: we analyzed the expression levels of mir-127 in 33 HCC and non-cancerous tissues using qRT-PCR. MiR-127 is downregulated in 69.7% of HCC tissues compared with adjacent normal tissues, but its expression level is not correlated with the TNM stage, AFP level, or age. In vitro, miR-127 can arrest Huh7 at the G2/M phase and inhibit Huh7 cell proliferation. In an in vivo xenograft model, the overexpression of miR-127 can inhibit Huh7 cell tumorigenicity. The luciferase reporter and western blot results confirm that miR-127 downregulates Sept7 expression by targeting its 3'UTR. Furthermore, the knockdown of Sept7 has the same effect on cell proliferation as the overexpression of miR-127 in Huh7 cells. Conclusion: miR-127 plays a tumor-suppressor role and can serve as a potential diagnostic biomarker for HCC.

show abstract

Section: Discussionsupporting

confidence: 92%

MicroRNA-127 Post-Transcriptionally Downregulates Sept7 and Suppresses Cell Growth in Hepatocellular Carcinoma Cells

Zhou

Yang

et al. 2014

Cell Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…SFRP1 is frequently downregulated in gliomas, but the cause of its downregulation is not well understood. Many investigators have reported that other miRNAs, such as miR-1260b, 127-3p, 206, and 139-5p, can inhibit cells' proliferation by targeting SFRP1 in prostate cancer, glioblastoma, gastric cancer, and breast cancer [24][25][26][27]. Other investigators showed that miR-1/206 regulates the SFRP1 gene and affects skeletal muscle development [28].…”

Section: Discussionmentioning

confidence: 99%

MiR-27a regulates Wnt/beta-catenin signaling through targeting SFRP1 in glioma

Wang

Xie²,

Xie³

et al. 2015

NeuroReport

View full text Add to dashboard Cite

Glioma is one of the most common intracranial tumors, and the prognosis is poor, although more and more treatments are employed. Wnt/beta-catenin signaling has been reported to be associated with glioma. SFRP1 acts as an antagonist and inhibits Wnt signaling by binding to Wnt molecules. In the present study, we aimed to investigate miRNA-27a as an antineoplastic factor that inhibits the Wnt/beta-catenin pathway by binding to the SFRP1 3'-UTR in glioma in vitro. We first showed that the expression of miR-27a was elevated in both glioma samples and cell lines. Furthermore, downregulation of miR-27a induced growth inhibition, cycle arrest, and apoptosis, and suppressed invasion/migration in glioma cell lines. Quantitative real-time PCR, western blot, and luciferase assay analysis showed that SFRP1 is a direct target of miR-27a. Overexpression of SFRP1 inhibited the malignancy of glioma cell lines. Our investigation showed that downregulation of miR-27a suppressed beta-catenin/TCF-4 transcription activity by targeting SFRP1. Our findings identify a role for miR-27a in glioma cell viability, cycle, apoptosis, and invasion/migration after activation of Wnt/beta-catenin signaling through SFRP1.

show abstract

“…miRNAs serve an important role in the progression of cancer, and a number of miRNAs are tumor suppressors, such as miR-127 (25). Inhibiting Tudor-SN promotes the expression of miR-127, and miR-127 has the ability to inhibit the migration of tumor cells (26); therefore, Tudor-SN is considered to have the ability to adjust the expression of AGPS .…”

Section: Discussionmentioning

confidence: 99%

Tudor‑staphylococcal nuclease regulates the expression and biological function of alkylglycerone phosphate synthase via nuclear factor‑κB and microRNA‑127 in human glioma U87MG cells

Zhang

Jia

et al. 2018

Oncol Lett

View full text Add to dashboard Cite

Abstract. Glioma is one of the malignant tumor types detrimental to human health; therefore, it is important to find novel targets and therapeutics for this tumor. The downregulated expression of Tudor-staphylococcal nuclease (SN) and alkylglycerone phosphate synthase (AGPS) can decrease cancer malignancy, and the overexpression of them can the increase viability and migration potential of various tumor cell types; however, the role of AGPS in the proliferation and migration of glioma, and the association of Tudor-SN and AGPS in human glioma is not clear. In the present study, it was determined that AGPS silencing suppressed the proliferation and migration potential of glioma U87MG cells, and suppressed the expression of the circular RNAs circ-ubiquitin-associated protein 2, circ-zinc finger protein 292 and circ-homeodomain-interacting protein kinase 3, and the long non-coding RNAs H19 imprinted maternally expressed transcript (non-protein coding), colon cancer-associated transcript 1 (non-protein coding) and hepatocellular carcinoma upregulated long non-coding RNA. Furthermore, Tudor-SN silencing suppressed the expression of AGPS; however, nuclear factor (NF)-κB and microRNA (miR)-127 retrieval experiments partially reduced the expression of AGPS. Additionally, it was determined that Tudor-SN silencing suppressed the activity of the mechanistic target of rapamycin (mTOR) signaling pathway, and NF-κB and miR-127 retrieval experiments partially reduced the activity of mTOR. Therefore, it was considered that NF-κB and miR-127 may be the mediators of Tudor-SN-regulated AGPS via the mTOR signaling pathway. These results improve on our knowledge of the mechanisms underlying Tudor-SN and AGPS in human glioma. IntroductionGliomas are a type of tumor formed by neoplastic transformation of neural stem cells, progenitor cells and differentiated glial cells, including astrocytes, oligodendrocytes and ependymal cells (1). Neoplastic cells may spread diffusely to normal brain tissues and damage normal neurological functions, which is the reason why malignant gliomas are detrimental to human health (2). In China, gliomas constitute 44.69% of primary intracranial tumors and 1-3% of generalized malignancies (3). According to the World Health Organization, malignant glioma causes the second highest amount of mortalities in sufferers <34 years old, and the third highest amount of mortalities in sufferers aged 35-54 years old (4). It is estimated that the survival time of the majority of glioma sufferers is ~1 year. Although there are currently various treatments available, including excision, chemotherapy and radiotherapy, the characteristic of strong invasiveness has severely influenced the effectiveness of glioma treatment.MicroRNAs (miRNAs/miRs) are an important molecular mediator of cell genetic changes, and are directly or indirectly associated with the occurrence and development of a number

show abstract

Next Generation Sequencing Analysis of miRNAs: MiR-127-3p Inhibits Glioblastoma Proliferation and Activates TGF-β Signaling by Targeting SKI

Cited by 45 publications

References 23 publications

MicroRNA-127 Post-Transcriptionally Downregulates Sept7 and Suppresses Cell Growth in Hepatocellular Carcinoma Cells

MicroRNA-127 Post-Transcriptionally Downregulates Sept7 and Suppresses Cell Growth in Hepatocellular Carcinoma Cells

MiR-27a regulates Wnt/beta-catenin signaling through targeting SFRP1 in glioma

Tudor‑staphylococcal nuclease regulates the expression and biological function of alkylglycerone phosphate synthase via nuclear factor‑κB and microRNA‑127 in human glioma U87MG cells

Contact Info

Product

Resources

About