Next-generation Raman tomography instrument for non-invasive in vivo bone imaging

Demers, Jennifer Lynn; Esmonde-White, Francis W. L.; Esmonde-White, Karen A.; Morris, Michael D.; Pogue, Brian W.

doi:10.1364/boe.6.000793

Cited by 54 publications

(42 citation statements)

References 31 publications

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“…[305] This is realized by arranging the excitation and collection fibers at certain distances.P otential applications include the noninvasive diagnosis of bone disease and cancer and monitoring glucose levels.Anext-generation Raman tomography instrument combined ten detection fibers and five excitation fibers. [306] Phantom measurements of hydroxyapatite concentrations from 50 to 300 mg mL À1 showed alinear response.Promising results from animal experiments paved the way for longitudinal measurements during fracture healing and scaling of Raman tomography towards measurements in humans.F irst in vivo noninvasive Raman spectra of rat tibiae were obtained using fiber-optic probes and holders designed for transcutaneous Raman measurements in small animals. [307] Thec onfiguration places multiple fibers around al eg,m aintaining contact with the skin.…”

Section: Angewandte Chemiementioning

confidence: 95%

Label‐Free Molecular Imaging of Biological Cells and Tissues by Linear and Nonlinear Raman Spectroscopic Approaches

et al. 2017

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Raman spectroscopy is an emerging technique in bioanalysis and imaging of biomaterials owing to its unique capability of generating spectroscopic fingerprints. Imaging cells and tissues by Raman microspectroscopy represents a nondestructive and label-free approach. All components of cells or tissues contribute to the Raman signals, giving rise to complex spectral signatures. Resonance Raman scattering and surface-enhanced Raman scattering can be used to enhance the signals and reduce the spectral complexity. Raman-active labels can be introduced to increase specificity and multimodality. In addition, nonlinear coherent Raman scattering methods offer higher sensitivities, which enable the rapid imaging of larger sampling areas. Finally, fiber-based imaging techniques pave the way towards in vivo applications of Raman spectroscopy. This Review summarizes the basic principles behind medical Raman imaging and its progress since 2012.

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Section: Angewandte Chemiementioning

confidence: 95%

Label‐Free Molecular Imaging of Biological Cells and Tissues by Linear and Nonlinear Raman Spectroscopic Approaches

et al. 2017

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“…The Raman measurements were able to detect the presence of heterotopic ossification in mouse models much earlier (5 days post surgery) than microCT (three weeks post surgery). SORS has also been combined with other complementary techniques such as optical coherence tomography for in-vivo bone measurement [408]. One recent development in SORS include non-invasive micro-SORS monitoring of blood donations stored in sterile plastic (PVC) blood-bags [409].…”

Section: Applicationsmentioning

confidence: 99%

Raman spectroscopy: techniques and applications in the life sciences

Shipp¹,

Sinjab²,

Notingher³

2017

Adv. Opt. Photon.

233

189

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Raman spectroscopy is an increasingly popular technique in many areas including biology and medicine.It is based on Raman scattering, a phenomenon in which incident photons lose or gain energy via interactions with vibrating molecules in a sample. These energy shifts can be used to obtain information regarding molecular composition of the sample with very high accuracy. Applications of Raman spectroscopy in the life sciences have included quantification of biomolecules, hyperspectral molecular imaging of cells and tissue, medical diagnosis, and others. This review briefly presents the physical origin of Raman scattering explaining the key classical and quantum mechanical concepts. Variations of the Raman effect will also be considered, including resonance, coherent, and enhanced Raman scattering. We discuss the molecular origins of prominent bands often found in the Raman spectra of biological samples. Finally, we examine several variations of Raman spectroscopy techniques in practice, looking at their applications, strengths, and challenges. This review is intended to be a starting resource for scientists new to Raman spectroscopy, providing theoretical background and practical examples as the foundation for further study and exploration.

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“…As such, chemical and structural information were not obtained for trabecular or endosteal surfaces. The periosteal surface was chosen for evaluation because it is the most likely surface to be probed via in vivo Raman spectroscopy when used as a transcutaneous or intraoperative screening tool to identify compromised tissue [38]. It is possible that PTH and irradiation have more pronounced effects at anatomic locations prone to more rapid bone turnover.…”

Section: Discussionmentioning

confidence: 99%

Parathyroid hormone attenuates radiation-induced increases in collagen crosslink ratio at periosteal surfaces of mouse tibia

Oest

Gong

Esmonde-White

et al. 2016

Bone

Self Cite

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As part of our ongoing efforts to understand underlying mechanisms contributing to radiation-associated bone fragility and to identify possible treatments, we evaluated the longitudinal effects of parathyroid hormone (PTH) treatment on bone quality in a murine model of limited field irradiation. We hypothesized PTH would mitigate radiation-induced changes in the chemical composition and structure of bone, as measured by microscope-based Raman spectroscopy. We further hypothesized that collagen crosslinking would be especially responsive to PTH treatment. Raman spectroscopy was performed on retrieved tibiae (6-7/group/time point) to quantify metrics associated with bone quality, including: mineral-to-matrix ratio, carbonate-to-phosphate ratio, mineral crystallinity, collagen crosslink (trivalent:divalent) ratio, and the mineral and matrix depolarization ratios. Irradiation disrupted the molecular structure and orientation of bone collagen, as evidenced by a higher collagen crosslink ratio and lower matrix depolarization ratio (vs. non-irradiated control bones), persisting until 12 weeks post-irradiation. Radiation transiently affected the mineral phase, as evidenced by increased mineral crystallinity and mineral-to-matrix ratio at 4 weeks compared to controls. Radiation decreased bone mineral depolarization ratios through 12 weeks, indicating increased mineral alignment. PTH treatment partially attenuated radiation-induced increases in collagen crosslink ratio, but did not restore collagen or mineral alignment. These post-radiation matrix changes are consistent with our previous studies of radiation damage to bone, and suggest that the initial radiation damage to bone matrix has extensive effects on the quality of tissue deposited thereafter. In addition to maintaining bone quality, preventing initial radiation damage to the bone matrix (i.e. crosslink ratio, matrix orientation) may be critical to preventing late-onset fragility fractures.

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Next-generation Raman tomography instrument for non-invasive in vivo bone imaging

Cited by 54 publications

References 31 publications

Label‐Free Molecular Imaging of Biological Cells and Tissues by Linear and Nonlinear Raman Spectroscopic Approaches

Label‐Free Molecular Imaging of Biological Cells and Tissues by Linear and Nonlinear Raman Spectroscopic Approaches

Raman spectroscopy: techniques and applications in the life sciences

Parathyroid hormone attenuates radiation-induced increases in collagen crosslink ratio at periosteal surfaces of mouse tibia

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