Next Generation of Voluntary PRRS Virus Regional Control Programs

Magalhães, Edison S.; Zimmerman, Jeffrey J.; Holtkamp, Derald; Classen, Dyneah M.; Groth, Douglas D.; Glowzenski, Lauren; Philips, Reid; Silva, Gustavo S.; Linhares, Daniel

doi:10.3389/fvets.2021.769312

Cited by 3 publications

(3 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The most frequently used population-based sample types are oral fluids ( 9 ), processing fluids ( 10 ), and family oral fluids ( 11 ). It has been reported that population-based methods increase herd sensitivity without increasing cost, time, and labor ( 10 , 12 ), compared to bleeding a subset of animals.…”

Section: Introductionmentioning

confidence: 99%

Porcine reproductive and respiratory syndrome virus RNA detection in tongue tips from dead animals

et al. 2022

Self Cite

View full text Add to dashboard Cite

The control of porcine reproductive and respiratory syndrome virus (PRRSV) hinges on monitoring and surveillance. The objective of this study was to assess PRRSV RNA detection by RT-PCR in tongue tips from dead suckling piglets compared to serum samples, processing fluids, and family oral fluids. Tongue tips and serum samples were collected from three PRRSV-positive breeding herd farms (farms A, B, and C) of three different age groups: newborns (<24 h), processing (2 to 7 days of age), and weaning (18 to 22 days of age). Additionally, processing fluids and family oral fluids were collected from 2–7 days of age and weaning age, respectively. In farms A and B, PRRSV RNA was detected in tongue tips from all age groups (100 and 95%, respectively). In addition, PRRSV RNA was detected in pooled serum samples (42 and 27%), processing fluids (100 and 50%), and family oral fluids (11 and 22%). Interestingly, the average Ct value from tongue tips was numerically lower than the average Ct value from serum samples in the newborn age. In farm C, PRRSV RNA was only detected in serum samples (60%) and family oral fluids (43%), both from the weaning age. Further, no PRRSV RNA was detected in tongue tips when pooled serum samples from the same age group tested PRRSV RNA-negative. Taken together, these results demonstrate the potential value of tongue tips for PRRSV monitoring and surveillance.

show abstract

Section: Introductionmentioning

confidence: 99%

Porcine reproductive and respiratory syndrome virus RNA detection in tongue tips from dead animals

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…Annually, up to 19% of Quebec swine breeding herds experience the introduction of at least one new PRRSV strain [18]. To proactively manage the disease, PRRS regional control and elimination (ARC&E) initiatives were established in Quebec [ 21 ], similar to what has been done elsewhere [ 1 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

A descriptive study on spatial and temporal distributions of genetic clusters of porcine reproductive and respiratory syndrome virus infecting pig sites in Quebec, Canada, between 2010 and 2019

Lambert,

Arsenault,

Côté

et al. 2024

Porc Health Manag

View full text Add to dashboard Cite

Background The wide diversity of porcine reproductive and respiratory syndrome virus (PRRSV) strains combined with incomplete heterologous cross-protection complicates the management of the disease at both the herd and the regional levels. The objectives of this study were to describe the spatial and temporal distribution of various PRRSV genetic clusters infecting pig sites in Quebec, Canada, and to compare PRRSV regional diversity of wild-type sequences over the years. Materials and methods A retrospective surveillance-based study was conducted on all pig sites which had PRRSV ORF5 sequences from field submissions transferred into the Laboratoire d'épidémiologie et de médecine porcine database from January 1, 2010 to December 31, 2019. A maximum likelihood phylogenetic tree inferred from multiple sequence alignment was used to identify genetic clusters. For each wild-type cluster gathering ≥ 15 sequences, the number of pig sites in which the cluster was detected per administrative region and per year were displayed on bubble charts and the spatiotemporal distribution of pig sites was illustrated using pie chart maps. A molecular analysis of variance was performed to compare PRRSV wild-type sequence diversity according to the administrative region for each year. Results A total of 32 wild-type clusters gathering 1653 PRRSV2 sequences from 693 pig sites were described. Each cluster was detected on up to 132 pig sites and 7 administrative regions over the 10-year period. Annually, the mean (min–max) number of wild-type clusters detected in at least one pig site reached 24 (17–29). Some clusters remained localized on a few sites over time whereas others were widespread over the territory during a few or many years. For each year, regional differences were also observed in PRRSV diversity of wild-type sequences. Conclusions The differences observed in both the spatiotemporal distributions of PRRSV clusters and in the regional diversity of wild-type sequences highlight the importance of ongoing provincial surveillance to improve collective PRRS management strategies.

show abstract

“…In the course of practical implementation of the measures in a farm, effective cooperation has to be established with the pig farm managers and the staff working in each production phase. Eventually, Hungary commenced PRRS eradication of the pig population based on the territorial principle in 2014 [ 10 , 11 ]. The aim of the legislated program was set to eliminate the causative agent of the PRRS disease, including the vaccine virus strains, and reach the PRRS-free status of all pigs.…”

Section: Introductionmentioning

confidence: 99%

Sampling Strategies in PRRS Elimination in Hungary: An Observational Study Involving Four Farrow-to-Finish Swine Herds

Fornyos,

Búza,

Makkai

et al. 2023

Veterinary Sciences

View full text Add to dashboard Cite

PRRS elimination strategies often rely on depopulation-repopulation. However, this approach is accompanied by a long-term loss of production. With adequate control measures, such as well-designed immunization programs and technological changes along with prevalence-based laboratory testing, the virus-free status of the most vulnerable age groups in swine herds can be achieved. The most common reason for acquiring PRRSV at large farrow-to-finish swine farm units is that the previously settled fattening pigs serve as a source of infection for the newly reared PRRS-free animals. Following such unwanted events, PRRSV may persist in an affected establishment for several years. In this observational study, we selected four farrow-to-finish type swine herds. We implemented different laboratory testing protocols to find the most optimal solution for a successful PRRS elimination program. To aid our objectives, we used a DIVA PCR technique. The PRRS DIVA PCR assay is a fast, reliable method to identify sows shedding farm-specific PRRSV strain(s). As a result of elimination efforts at the sentinel pig herds, we found that reliable detection of wild-type PRRSV shedding among sows requires sampling at least three weaned piglets per litter. The strict adherence to this sampling protocol, the systematic use of laboratory methods that quickly detect the presence of wild virulent virus in the herd during the rearing period and the culling of DIVA PCR positive litters and their sows decreased the presence of the resident virus markedly. These procedures at Hungarian farrow-to-finish type farms successfully inhibited the wild-type PRRSV infection of different age groups. The results of this study demonstrate that applying this methodology together with strict biosecurity measures enabled us to reach PRRS-vaccinated-free status in large, farrow-to-finish herds within two years.

show abstract

Next Generation of Voluntary PRRS Virus Regional Control Programs

Cited by 3 publications

References 23 publications

Porcine reproductive and respiratory syndrome virus RNA detection in tongue tips from dead animals

Porcine reproductive and respiratory syndrome virus RNA detection in tongue tips from dead animals

A descriptive study on spatial and temporal distributions of genetic clusters of porcine reproductive and respiratory syndrome virus infecting pig sites in Quebec, Canada, between 2010 and 2019

Sampling Strategies in PRRS Elimination in Hungary: An Observational Study Involving Four Farrow-to-Finish Swine Herds

Contact Info

Product

Resources

About