Next generation DNA sequencing reveals allopolyploid origin of decaploid Isoëtes lacustris (Isoëtaceae)

Grigoryan, Maxim Yu.; Bobrov, Alexander A.; Brunton, Daniel F.; Volkova, Polina A.; Logacheva, Maria D.; Neretina, Tatyana V.

doi:10.1016/j.aquabot.2020.103326

Cited by 9 publications

(9 citation statements)

References 38 publications

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“…A submerged species of acidic substrate in lakes in the boreal forest region of Manitoba (Figure 3r). Very rare and S2; possibly disjunct from a predominately eastern range (Brunton and Britton 1991;Grigoryan et al 2020). Known from six specimens (Table 1).…”

Section: Isoetaceae Quillwortsmentioning

confidence: 99%

A synopsis of lycophytes in Manitoba, Canada: their status, distribution, abundance, and habitats

Staniforth

Brunton²

2022

Can Field Nat

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A steady increase in the number of lycophyte taxa discovered in Manitoba over the last 20 years prompted a determination of which species should be included in an updated provincial list. Collections made throughout the province since 2008 and a critical examination of over 1000 herbarium specimens enabled a substantive review and update of Manitoba’s lycophyte flora. It now comprises 22 taxa: 14 species and two hybrid clubmosses (Lycopodiaceae), three spikemosses (Selaginellaceae), and two species and one hybrid quillwort (Isoetaceae). Thirteen of the 21 species are designated to be of conservation concern, with NatureServe ranks of Critically Imperilled (S1; three), Imperilled (S2; two), or Vulnerable (S3; nine). Based on verified specimens, we describe the abundance and habitats, and summarize recent changes in nomenclature and systematics for all Manitoba lycophyte taxa.

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Section: Isoetaceae Quillwortsmentioning

confidence: 99%

A synopsis of lycophytes in Manitoba, Canada: their status, distribution, abundance, and habitats

Staniforth

Brunton²

2022

Can Field Nat

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“…but I hadn't yet the opportunity to apply it to butterworts. However, independent similar experiments gave interesting results on ferns (Rothfels et al 2017), moonworts (Dauphin et al 2018) and quillworts (Grigoryan et al 2020), for example.…”

Section: Additional Comments and Discussionmentioning

confidence: 92%

Additional features for the recently described Pinguicula (Lentibulariaceae) species from Spain

Roccia¹

2021

Carniv. Pl. Newslett.

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Two new butterwort species, Pinguicula casperiana and P. tejedensis, from Central Spain have been recently described, and the name P. submediterranea has been resurrected in the same publication. My article describing “P. conquensis” was under review at that time and this plant is exactly the same as P. casperiana. As it gives additional morphological diagnostic characters, it is worth bringing it to everyone’s attention for a better understanding of this species. Additional comments are made about P. submediterranea and P. tejedensis as those populations were also studied in my article: those names are here considered later synonyms of P. dertosensis. Pinguicula casperiana raises to eleven the number of Pinguicula species from Spain, being the sixth endemic to the Iberian Peninsula.

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“…In our case, we studied the intragenomic variability of the transcribed spacer ITS1 using Illumina high-throughput next-generation sequencing (NGS). This approach is suitable for revealing the hidden polymorphism of hybrid taxa in the case of multiple crossing [10][11][12]. In addition, we took into analysis ITS1-5.8S rDNA-ITS2 sequences of the nuclear genome, as well as trnL-trnF and trnK-rps16 sequences of the chloroplast genome, obtained by the Sanger method.…”

Section: Introductionmentioning

confidence: 99%

Origin of the Rare Hybrid Genus ×Trisetokoeleria Tzvelev (Poaceae) According to Molecular Phylogenetic Data

Gnutikov

Носов

Королева

et al. 2022

Plants

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In our article, we analyzed new data on the origin of the hybrid genus ×Trisetokoeleria. According to the morphological criteria ×T. jurtzevii is a hybrid between Koeleria asiatica s. l. and Trisetum spicatum, ×T. taimyrica, and originated from Koeleria asiatica s. l. and Trisetum subalpestre, ×T. gorodkowii, a hybrid between Koeleria asiatica and Trisetum ruprechtianum. Later ×T. taimyrica was transferred to Koeleria. Parental taxa are prone to active hybridization themselves, thus, new methods of next-generation sequencing (NGS) were needed to clarify the relationships of these genera. For NGS we used the fragment 18S rDNA (part)–ITS1–5.8S rDNA (totally 441 accessions). We analyzed ITS1–5.8S rDNA–ITS2 region, trnL–trnF and trnK–rps16 from eight samples of the five species, using the Sanger method: ×Trisetokoeleria jurtzevii, ×T. taimyrica, Koeleria asiatica, Sibirotrisetum sibiricum (=Trisetum sibiricum), and Trisetum spicatum. We also studied the pollen fertility of ×Trisetokoeleria and its possible progenitors. Our data partly contradicted previous assumptions, based on morphological grounds, and showed us a picture of developed introgression within and between Koeleria and Trisetum. ×T. jurtzevii, a totally sterile hybrid formed rather recently. We can suppose that ×T. jurtzevii is a hybrid between K. asiatica and some Trisetum s. str. Species, but not T. spicatum. ×T. gorodkowii, a hybrid in the stage of primary stabilization; it has one unique ribotype related to T. spicatum s. l. The second parental species is unrelated to Trisetum ruprechtianum. ×T. taimyrica and is a stabilized hybrid species; it shares major ribotypes with the T. spicatum/T. wrangelense group and has a minor fraction of rDNA related to genus Deyeuxia s. l.

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Next generation DNA sequencing reveals allopolyploid origin of decaploid Isoëtes lacustris (Isoëtaceae)

Cited by 9 publications

References 38 publications

A synopsis of lycophytes in Manitoba, Canada: their status, distribution, abundance, and habitats

A synopsis of lycophytes in Manitoba, Canada: their status, distribution, abundance, and habitats

Additional features for the recently described Pinguicula (Lentibulariaceae) species from Spain

Origin of the Rare Hybrid Genus ×Trisetokoeleria Tzvelev (Poaceae) According to Molecular Phylogenetic Data

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