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Houten, Sander M.

doi:10.1007/s10545-017-0062-2

Cited by 4 publications

(5 citation statements)

References 3 publications

(4 reference statements)

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“…[117] Concentrations of fatty acids that exceed the maximal β-oxidation capacity are toxic to mitochondria and contribute to mitochondrial dysfunction. [118] As mentioned above, the studies by Cao et al and Yan et al showed that punicalagin, one of the important active ingredients of pomegranate, effectively alleviates mitochondrial damage in vivo and in vitro. From these points of view, mitochondria are more likely to be the targets of pomegranate extracts in the regulation of lipid metabolism in metabolic disorders.…”

Section: Mechanism Analysis Of Lipid Metabolism Regulated By Pomegranatementioning

confidence: 94%

Beneficial Effects of Pomegranate on Lipid Metabolism in Metabolic Disorders

Hou

Zhang

et al. 2019

Molecular Nutrition Food Res

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Pomegranate (Punica granatum Linn) is used in the prevention and treatment of metabolic syndrome in recent decades. Imbalances in lipid metabolism are profound features of metabolic disorders. In vivo and in vitro studies have shown that extracts of different pomegranate fractions (peels, flowers, juice, and seeds) regulate lipid metabolism in metabolic‐disorder‐associated diseases such as atherosclerosis, nonalcoholic fatty liver disease, and type 2 diabetes, helping to alleviate the development of diseases. Amelioration of oxidative stress and the inflammatory response is considered an important reason underlying the regulation of lipid metabolism by pomegranate extracts. Mitochondria, the major cellular site for lipid oxidation, are strongly associated with cellular oxidative and inflammatory status and are likely to be a target for pomegranate extract action. This review summarizes the main findings about the effects of different pomegranate fraction extracts on lipid metabolism in metabolic‐disorder‐associated diseases and analyses how pomegranate extracts achieve their effects. Furthermore, it also provides an important basis for the research and development of pomegranate‐related nutrients or drugs.

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Section: Mechanism Analysis Of Lipid Metabolism Regulated By Pomegranatementioning

confidence: 94%

Beneficial Effects of Pomegranate on Lipid Metabolism in Metabolic Disorders

Hou

Zhang

et al. 2019

Molecular Nutrition Food Res

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“…53 The pathway is also known for the synthesis of acetyl CoA, NADH, and FADH2. 54 Ascorbic acid (vitamin C), which is an essential part of a regular diet, plays a critical role in the maintenance of a normal mature collagen network in humans by preventing the auto-inactivation of lysyl and prolyl hydroxylase, two key enzymes in collagen biosynthesis. 55 Proline also plays an important role in collagen formation.…”

Section: Discussionmentioning

confidence: 99%

“…It is involved in the metabolism of fatty acids, which bind to coenzyme A to form fatty acyl CoA thioesters 53 . The pathway is also known for the synthesis of acetyl CoA, NADH, and FADH2 54 …”

Section: Discussionmentioning

confidence: 99%

A comprehensive analysis of metabolomics and lipidomics in areca nut mediated oral submucous fibrosis progression through LCMS and Raman spectroscopy

Verma,

Adhikari,

Singh

et al. 2024

J Raman Spectroscopy

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Habitual consumption of areca/betel nut in Southeast Asia has been associated with oral submucous fibrosis (OSMF) and its malignant transformation to oral squamous cell carcinoma. The current study aimed to assess the molecular alterations in fibroblast cell lines after treatment with areca nut. Areca nut extract (ANE) was prepared and characterized for its active ingredient, arecoline. ANE‐treated cells were subjected to cell viability, proliferation, migration, morphologic changes, and transcript of OSMF genes (col1a1, col1a2, hsp47, timp1, timp2, timp3, and timp4). Further, liquid chromatography–mass spectrometry (LCMS) of cell lysate and Raman microspectroscopy (RMS) of fixed cells were performed for metabolomics/lipidomics and biomolecular alterations in the nucleus and periphery of ANE‐treated cells. We compared and integrated the data from both techniques and observed that the cells treated with ANE mimicked OSMF and could be used as an in vitro model. LCMS showed a significant alteration in 17 metabolites and 165 lipid molecules in the cells treated with ANE. Further, 40 molecules in the nucleus and 29 in the periphery were found to be altered in these cells when subjected to RMS. Molecules associated with pathways such as the transfer of acetyl groups into mitochondria, amino sugar metabolism, and the Warburg effect were modulated the most upon ANE treatment. Acetyl CoA was found to be common in most of the altered pathways. Besides, the pathways affecting carbohydrate metabolism were also altered significantly. Targeting these molecules and pathways can help to prevent the malignant transformation of OSMF.

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“…Lipids serve a myriad of roles in biology; as catabolic carbon source [1] , components of cellular and organellar membranes [2] , post-translational protein modifications [3] , and signalling molecules [4] . The study of their contributions in biology is complicated by their lack of direct genetic encoding, their inherent lipophilicity, and the fact that chemical modifications, e.g.…”

Section: Introductionmentioning

confidence: 99%

Live‐Cell Imaging of Sterculic Acid—a Naturally Occurring 1,2‐Cyclopropene Fatty Acid—by Bioorthogonal Reaction with Turn‐On Tetrazine‐Fluorophore Conjugates**

et al. 2022

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In the field of lipid research, bioorthogonal chemistry has made the study of lipid uptake and processing in living systems possible, whilst minimising biological properties arising from detectable pendant groups. To allow the study of unsaturated free fatty acids in live cells, we here report the use of sterculic acid, a 1,2-cyclopropene-containing oleic acid analogue, as a bioorthogonal probe. We show that this lipid can be readily taken up by dendritic cells without toxic side effects, and that it can subsequently be visualised using an inverse electron-demand Diels-Alder reaction with quenched tetrazine-fluorophore conjugates. In addition, the lipid can be used to identify changes in protein oleoylation after immune cell activation. Finally, this reaction can be integrated into a multiplexed bioorthogonal reaction workflow by combining it with two sequential copper-catalysed Huisgen ligation reactions. This allows for the study of multiple biomolecules in the cell simultaneously by multimodal confocal imaging.

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News and views

Cited by 4 publications

References 3 publications

Beneficial Effects of Pomegranate on Lipid Metabolism in Metabolic Disorders

Beneficial Effects of Pomegranate on Lipid Metabolism in Metabolic Disorders

A comprehensive analysis of metabolomics and lipidomics in areca nut mediated oral submucous fibrosis progression through LCMS and Raman spectroscopy

Live‐Cell Imaging of Sterculic Acid—a Naturally Occurring 1,2‐Cyclopropene Fatty Acid—by Bioorthogonal Reaction with Turn‐On Tetrazine‐Fluorophore Conjugates**

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