Newly Obtained Apple Pectin as an Adjunct to Irinotecan Therapy of Colorectal Cancer Reducing E. coli Adherence and β-Glucuronidase Activity

Palko-Łabuz, Anna; Maksymowicz, Jerzy; Sobieszczańska, Beata; Wikiera, Agnieszka; Skonieczna, Magdalena; Wesołowska, Olga; Środa-Pomianek, Kamila

doi:10.3390/cancers13122952

Cited by 22 publications

(24 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The gut microbiome decomposes irinotecan-metabolite back to the active form, causing side effects. A new therapy, together with apple pectin, was able to inhibit metabolites responsible for diarrhoea in cell culture with human colorectal tumour cells and at the same time enhance the cytotoxic effect of irinotecan [78]. However, nonresponse to chemotherapy may also represent a complication of chemotherapy.…”

Section: Discussionmentioning

confidence: 99%

The Association of Gut Microbiota and Complications in Gastrointestinal-Cancer Therapies

et al. 2021

View full text Add to dashboard Cite

The therapy of gastrointestinal carcinomas includes surgery, chemo- or immunotherapy, and radiation with diverse complications such as surgical-site infection and enteritis. In recent years, the microbiome’s influence on different diseases and complications has been studied in more detail using methods such as next-generation sequencing. Due to the relatively simple collectivisation, the gut microbiome is the best-studied so far. While certain bacteria are sometimes associated with one particular complication, it is often just the loss of alpha diversity linked together. Among others, a strong influence of Fusobacterium nucleatum on the effectiveness of chemotherapies is demonstrated. External factors such as diet or specific medications can also predispose to dysbiosis and lead to complications. In addition, there are attempts to treat developed dysbiosis, such as faecal microbiota transplant or probiotics. In the future, the underlying microbiome should be investigated in more detail for a better understanding of the precipitating factors of a complication with specific therapeutic options.

show abstract

Section: Discussionmentioning

confidence: 99%

The Association of Gut Microbiota and Complications in Gastrointestinal-Cancer Therapies

et al. 2021

View full text Add to dashboard Cite

show abstract

“…In contrast, the viability of normal mouse fibroblasts was not affected by pectins in the studied concentrations. Recently, cellulase-extracted apple pectin, PC, was demonstrated to reduce the viability of colon cancer cells [20]. Pectins isolated from other plant sources, such as potato [26] and ginseng [27], were previously shown to also be anticancer agents in human colorectal adenocarcinoma HT-29 cells.…”

Section: Discussionmentioning

confidence: 99%

“…In our previous study [20], the promising anticancer activity of apple pectin PC, obtained with the use of endo-cellulase, was demonstrated. Its anticancer properties in colon cancer cell line HCT 116 were shown to be superior to commercially available modified citrus pectin, PectaSol.…”

Section: Introductionmentioning

confidence: 98%

The Use of Endo-Cellulase and Endo-Xylanase for the Extraction of Apple Pectins as Factors Modifying Their Anticancer Properties and Affecting Their Synergy with the Active Form of Irinotecan

et al. 2022

Self Cite

View full text Add to dashboard Cite

Pectin constitutes an essential component of dietary fiber. Modified pectins from various sources possess potent anticancer and immunomodulatory activities. In this study, two pectins isolated from apple pomace by Trichoderma enzyme treatment, PX (with endo-xylanase) and PCX (with both endo-cellulase and endo-xylanase), were studied in colon cancer cell lines (HCT 116, Caco-2, and HT-29). Both pectins reduced colon cancer cell viability, induced apoptosis, and increased intracellular amounts of reactive oxygen species. Additionally, synergy between pectin and an active form of irinotecan, SN-38, in all aspects mentioned above, was discovered. This drug is a common component of cytotoxic combinations recommended as treatment for colon cancer patients. PX and PCX demonstrated significant anti-inflammatory activity in lipopolysaccharide-stimulated cells. Interaction of apple pectins with galectin-3 and Toll-like Receptor 4 (TLR4) was suggested to be responsible for their anticancer and anti-inflammatory effect. Since PCX was more active than PX in almost all experiments, the role of the enzyme used to obtain the pectin for its biological activity was discussed. It was concluded that co-operation between both enzymes was needed to obtain the molecule of the most beneficial properties. The low molecular mass of PCX together with a high proportion of rhamnogalacturonan I (RG I) regions seemed to be crucial for its superior activity.

show abstract

“…The anti-tumor effects of dietary pectin may be boosted by formulating the fiber with a chemo-protective food component [69,70]. The mechanisms underlying this effect are associated with modulation of gut microbiota [71], apoptosis [72,73], tumor cell growth [74], and miRNA expression [67]. The anti-tumor efficacy of pectins have been primarily investigated in colon cancer.…”

Section: Pectinmentioning

confidence: 99%

“…Pectin supplementation facilitated an anti-PD-mAb effect in humanized C57BL/6 mice harboring colon cancer patient cells by enhancing T cell infiltration into the tumor microenvironment [71]. A study explored the possibility of its use as an adjuvant in irinotecan therapy for both enhancing curative efficacy and ameliorating the side-effects of colon cancer treatment [72]. In the study, a novel enzymatically-extracted apple pectin was shown to induce apoptosis and ROS production, while reducing cell viability and preventing the adhesion of prototype adherent-invasive E. coli in colorectal cancer cells, and HCT116 and Caco-2 cells.…”

Section: Pectinmentioning

confidence: 99%

The Anti-Cancer Effects of Red-Pigmented Foods: Biomarker Modulation and Mechanisms Underlying Cancer Progression

2022

View full text Add to dashboard Cite

Cancer is one the most malignant diseases that is a leading cause of death worldwide. Vegetables and fruits contain beneficial nutrients such as vitamins, minerals, folates, dietary fibers, and various natural bioactive compounds. These can prevent the pathological processes of many cancers and reduce cancer related mortality. Specifically, the anti-cancer effect of vegetables and fruits is largely attributable to the natural bioactive compounds present within them. A lot of bioactive compounds have very specific colors with pigments and the action of them in the human body varies by their color. Red-pigmented foods, such as apples, oranges, tomatoes, cherries, grapes, berries, and red wine, have been widely reported to elicit beneficial effects and have been investigated for their anti-tumor, anti-inflammatory, and antioxidative properties, as well as anti-cancer effect. Most of the anti-cancer effects of bioactive compounds in red-pigmented foods arise from the suppression of cancer cell invasion and metastasis, as well as the induction of apoptosis and cell cycle arrest. In this review, we assessed publications from the last 10 years and identified 10 bioactive compounds commonly studied in red-pigmented foods: lycopene, anthocyanin, β-carotene, pectin, betaine, rutin, ursolic acid, kaempferol, quercetin, and myricetin. We focused on the mechanisms and targets underlying the anti-cancer effect of the compounds and provided rationale for further investigation of the compounds to develop more potent anti-cancer treatment methods.

show abstract

Newly Obtained Apple Pectin as an Adjunct to Irinotecan Therapy of Colorectal Cancer Reducing E. coli Adherence and β-Glucuronidase Activity

Cited by 22 publications

References 75 publications

The Association of Gut Microbiota and Complications in Gastrointestinal-Cancer Therapies

The Association of Gut Microbiota and Complications in Gastrointestinal-Cancer Therapies

The Use of Endo-Cellulase and Endo-Xylanase for the Extraction of Apple Pectins as Factors Modifying Their Anticancer Properties and Affecting Their Synergy with the Active Form of Irinotecan

The Anti-Cancer Effects of Red-Pigmented Foods: Biomarker Modulation and Mechanisms Underlying Cancer Progression

Contact Info

Product

Resources

About