Newly isolated phages preying on Pseudomonas syringae pv. garcae: In vitro and ex vivo inactivation studies in coffee plant leafs

Silva, Erica C.; Rodrigues, Lucas M.R.; Vila, Marta M.D.C.; Balcão, Victor M.

doi:10.1016/j.enzmictec.2023.110325

Cited by 5 publications

(15 citation statements)

References 82 publications

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“…As a result, our collection has greater intrinsic diversity due to the lower number of phages per genus and the higher number of genera. For our study, we hypothesized that a high diversity of P. syringae phages would require a large number of different enrichment strains compared to other studies that typically used only one or two bacterial strains [25, 26, 50]. Yet, to increase the diversity of P. syringae phages with an efficient sampling effort, it seems that using at least three enrichment strains (instead of 21) from a single geographical site would enable the collection of many distinct genera of P. syringae phages.…”

Section: Resultsmentioning

confidence: 99%

Novel phages of Pseudomonas syringae unveil numerous potential auxiliary metabolic genes

Feltin,

Garneau,

Morris

et al. 2024

Journal of General Virology

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Relatively few phages that infect plant pathogens have been isolated and investigated. The Pseudomonas syringae species complex is present in various environments, including plants. It can cause major crop diseases, such as bacterial canker on apricot trees. This study presents a collection of 25 unique phage genomes that infect P. syringae. These phages were isolated from apricot orchards with bacterial canker symptoms after enrichment with 21 strains of P. syringae. This collection comprises mostly virulent phages, with only three being temperate. They belong to 14 genera, 11 of which are newly discovered, and 18 new species, revealing great genetic diversity within this collection. Novel DNA packaging systems have been identified bioinformatically in one of the new phage species, but experimental confirmation is required to define the precise mechanism. Additionally, many phage genomes contain numerous potential auxiliary metabolic genes with diversified putative functions. At least three phages encode genes involved in bacterial tellurite resistance, a toxic metalloid. This suggests that viruses could play a role in bacterial stress tolerance. This research emphasizes the significance of continuing the search for new phages in the agricultural ecosystem to unravel novel ecological diversity and new gene functions. This work contributes to the foundation for future fundamental and applied research on phages infecting phytopathogenic bacteria.

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Section: Resultsmentioning

confidence: 99%

Novel phages of Pseudomonas syringae unveil numerous potential auxiliary metabolic genes

Feltin,

Garneau,

Morris

et al. 2024

Journal of General Virology

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“…The phage particles were centrifuged in a micro-ultracentrifuge from Beckman-Coulter (model Optima TLX, Indianapolis, IN, USA) for 150 min at 45,000 rpm and 4 °C. The samples were negatively stained employing uranyl acetate at 2% ( w / v ) and pH 7.0, [ 21 , 22 ] and photomicrographed in a Transmission Electron Microscope from JEOL (model JEM 2100, Tokyo, Japan) using a high-resolution CCD camera from GATAN Inc. (model ORIUS™ 832.J4850 SC1000B, Pleasanton, CA, USA). The software Gatan Microscopy Suite (DigitalMicrograph from GATAN Inc., version 2.11.1404.0) was used to obtain digital photos of the phage virions.…”

Section: Methodsmentioning

confidence: 99%

An Edible Antibacterial Coating Integrating Lytic Bacteriophage Particles for the Potential Biocontrol of Salmonella enterica in Ripened Cheese

Vila,

Cinto,

Pereira

et al. 2024

Polymers

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The goal of this research was to create an antibacterial biopolymeric coating integrating lytic bacteriophages against Salmonella enterica for use in ripened cheese. Salmonella enterica is the main pathogen that contaminates food products and the food industry. The food sector still uses costly and non-selective decontamination and disease control methods. Therefore, it is necessary to look for novel pathogen biocontrol technologies. Bacteriophage-based biocontrol seems like a viable option in this situation. The results obtained show promise for food applications since the edible packaging developed (EdiPhage) was successful in maintaining lytic phage viability while preventing the contamination of foodstuff with the aforementioned bacterial pathogen.

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“…As a result, our collection has greater intrinsic diversity due to the lower number of phages per genus and the higher number of genera. For our study, we hypothesised that a high diversity of P. syringae phages would require a large number of different enrichment strains compared to other studies that typically used only one or two bacterial strains [25,26,50]. Yet, to increase the diversity of P. syringae phages with an efficient sampling effort, it seems that using at least 3 enrichment strains (instead of 21) from a single geographical site would enable the collection of many distinct genera of P. syringae phages.…”

Section: P Syringae Phages From Apricot Soils Represent 11 New Genera...mentioning

confidence: 99%

Novel phages ofPseudomonas syringaeunveil numerous potential auxiliary metabolic genes

Feltin,

Garneau,

Morris

et al. 2024

Preprint

View full text Add to dashboard Cite

Relatively few phages that infect plant pathogens have been isolated and investigated. The Pseudomonas syringae species complex is present in various environments, including plants. It can cause major crop diseases, such as bacterial canker on apricot trees. This study presents a collection of 25 unique phages genomes that infect P. syringae. These phages were isolated from apricot orchards with bacterial canker symptoms after enrichment with 21 strains of P. syringae. This collection comprises mostly virulent phages, with only three being temperate. They belong to 14 genera, 11 of which are newly discovered, and 18 new species, revealing great genetic diversity within this collection. Novel DNA packaging systems have been identified bioinformatically in one of the new phage species, but experimental confirmation is required to define the precise mechanism. Additionally, many phage genomes contain numerous potential auxiliary metabolic genes with diversified putative functions. At least three phages encode genes involved in bacterial tellurite resistance, a toxic metalloid. This suggests that viruses could play a role in bacterial stress tolerance. This research emphasises the significance of continuing the search for new phages in the agricultural ecosystem to unravel novel ecological diversity and new gene functions. This work contributes to the foundation for future fundamental and applied research on phages infecting phytopathogenic bacteria.

show abstract

Newly isolated phages preying on Pseudomonas syringae pv. garcae: In vitro and ex vivo inactivation studies in coffee plant leafs

Cited by 5 publications

References 82 publications

Novel phages of Pseudomonas syringae unveil numerous potential auxiliary metabolic genes

Novel phages of Pseudomonas syringae unveil numerous potential auxiliary metabolic genes

An Edible Antibacterial Coating Integrating Lytic Bacteriophage Particles for the Potential Biocontrol of Salmonella enterica in Ripened Cheese

Novel phages ofPseudomonas syringaeunveil numerous potential auxiliary metabolic genes

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