Newly Isolated Animal Pathogen Corynebacterium silvaticum Is Cytotoxic to Human Epithelial Cells

Möller, Jens; Busch, Anne; Berens, Christian; Hotzel, Helmut; Burkovski, Andreas

doi:10.3390/ijms22073549

Cited by 11 publications

(6 citation statements)

References 59 publications

(84 reference statements)

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“…За последние несколько лет число зарегистрированных случаев заболеваний, обусловленных Сorynebacterium spp., значительно увеличилось, что связывают с совершенствованием методов микробиологического исследования, направленных на точную идентификацию этих микроорганизмов [3]. Учитывая имеющиеся указания на роль в патологии недифтерийных коринебактерий, важное значение имеет установление параметров их патогенности, связанных с иными, помимо токсина, факторами (адгезивность, инвазивность, цитотоксичность, способность ряда видов продуцировать ферменты гемолизин и уреазу) [15,16]. Учитывая, что в настоящее время изоляты микроорганизмов с множественной лекарственной устойчивостью (МЛУ) расценивают как высоко патогенные [17], особую важность приобретает определение резистентности штаммов Сorynebaсterium spp.…”

Section: Rostov-on-don Plague Control Research Institute; 344002 Rost...unclassified

Corynebacterium Spp.: Relationship of Pathogenic Properties and Antimicrobial Resistance

Mangutov

Алиева

Харсеева

et al. 2023

RCLD

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Corynebacterium spp. are part of the human microbiome, but can cause the development of inflammatory diseases of various localization. Purpose - to evaluate the relationship between pathogenic properties and resistance to antimicrobial drugs (AMD) of Corynebacterium spp. from patients with inflammatory diseases of the respiratory tract. Strains of Corynebacterium spp. isolated from patients with inflammatory diseases of the respiratory tract (99 pcs.) and practically healthy individuals (33 pcs.). Isolates were identified by mass spectrometric method (MALDI-ToFMS), their adhesive and invasive activity on Hep-2 cells, cytopathic effect (CPE) in CHO-K1 cell culture, and resistance to antimicrobial drugs (AMD) were determined. Indicators of adhesion (3.65±0.679(CFU±m)x102/ml), invasion (1.72±0.230 (CFU±m)x102/ml), cytotoxicity (69.1±3.8% of dead CHO-K1 cells ) Corynebasterium spp. strains isolated from patients are higher (p≤0.05) than similar indicators in practically healthy people. 90.9% of isolates from patients had resistance to AMD, in most cases (57.6±4.9%) resistance to only one AMP was noted, less often to two (25.2±4.3%), three or more (8.08±2.7%). According to the results of correlation-regression analysis, pathogenic properties (adhesiveness, invasiveness, cytotoxicity) of Corynebacterium spp. strains isolated from patients are in close direct relationship with resistance to AMD. This indicates the importance of identifying strains of non-diphtheria corynebacteria resistant to AMDs, which, under the influence of developing resistance to AMDs, can increase their pathogenic potential, moving from commensalism to parasitism.

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Section: Rostov-on-don Plague Control Research Institute; 344002 Rost...unclassified

Corynebacterium Spp.: Relationship of Pathogenic Properties and Antimicrobial Resistance

Mangutov

Алиева

Харсеева

et al. 2023

RCLD

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“…While in principle less detrimental, even the NTTB strain W25 was cytotoxic to human epithelial cells and in the invertebrate model systems Caenorhabditis elegans and Galleria mellonella [ 53 ]. In the frame of a first proteome study of this species, the whole cell and cell surface fraction as well as the exoproteome of strain W25 were analyzed [ 54 ].…”

Section: Relevance and Properties Of Toxigenic Corynebacteriamentioning

confidence: 99%

Proteomics of Toxigenic Corynebacteria

Burkovski

2023

Proteomes

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Within the genus Corynebacterium, six species are potential carriers of the tox gene, which encodes the highly potent diphtheria exotoxin: Corynebacterium diphtheriae, Corynebacterium belfantii, Corynebacterium rouxii, Corynebacterium ulcerans, Corynebacterium pseudotuberculosis and Corynebacterium silvaticum. Based on their potential to infect different host species and cause either human infections, zoonotic diseases or infections of economically important animals, these bacteria are of high scientific and economic interest and different research groups have carried out proteome analyses. These showed that especially the combination of MS-based proteomics with bioinformatic tools helped significantly to elucidate the functional aspects of corynebacterial genomes and to handle the genome and proteome complexity. The combination of proteomic and bioinformatic approaches was also used to discover new vaccine and drug targets. In addition, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry has been established as a fast and precise tool for the identification of these bacteria.

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“…C. rouxii causes chronic arteritis leading to ulcerations on feet and legs, and peritonitis ( Badell et al, 2020 ). C. silvaticum has only been isolated from pigs and roe deer to date, causing caseous lymphadenitis ( Dangel et al, 2020 ), and is cytotoxic for human epithelial cells ( Möller et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Evidence of episodic positive selection in Corynebacterium diphtheriae complex of species and its implementations in identification of drug and vaccine targets

Viana

Profeta

Cerqueira

et al. 2022

PeerJ

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Background Within the pathogenic bacterial species Corynebacterium genus, six species that can produce diphtheria toxin (C. belfantii, C. diphtheriae, C. pseudotuberculosis, C. rouxii, C. silvaticum and C. ulcerans) form a clade referred to as the C. diphtheria complex. These species have been found in humans and other animals, causing diphtheria or other diseases. Here we show the results of a genome scale analysis to identify positive selection in protein-coding genes that may have resulted in the adaptations of these species to their ecological niches and suggest drug and vaccine targets. Methods Forty genomes were sampled to represent species, subspecies or biovars of Corynebacterium. Ten phylogenetic groups were tested for positive selection using the PosiGene pipeline, including species and biovars from the C. diphtheria complex. The detected genes were tested for recombination and had their sequences alignments and homology manually examined. The final genes were investigated for their function and a probable role as vaccine or drug targets. Results Nineteen genes were detected in the species C. diphtheriae (two), C. pseudotuberculosis (10), C. rouxii (one), and C. ulcerans (six). Those were found to be involved in defense, translation, energy production, and transport and in the metabolism of carbohydrates, amino acids, nucleotides, and coenzymes. Fourteen were identified as essential genes, and six as virulence factors. Thirteen from the 19 genes were identified as potential drug targets and four as potential vaccine candidates. These genes could be important in the prevention and treatment of the diseases caused by these bacteria.

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Newly Isolated Animal Pathogen Corynebacterium silvaticum Is Cytotoxic to Human Epithelial Cells

Cited by 11 publications

References 59 publications

Corynebacterium Spp.: Relationship of Pathogenic Properties and Antimicrobial Resistance

Corynebacterium Spp.: Relationship of Pathogenic Properties and Antimicrobial Resistance

Proteomics of Toxigenic Corynebacteria

Evidence of episodic positive selection in Corynebacterium diphtheriae complex of species and its implementations in identification of drug and vaccine targets

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