Newly Discovered Properties of Spore-Forming Sulfate-Reducing Bacteria, Desulfotomaculum Strains 435 and 781

Belyakova, E. V.; Rozanova, E. P.

doi:10.1023/b:mici.0000023993.95884.29

Cited by 4 publications

(8 citation statements)

References 5 publications

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“…However, a previous study reports Desulfotomaculum isolates in a salt pan [ 72 ]. Sulfate-reducing bacteria in Peptococcaceae perform oxidation from a broad spectrum of electron donors such as lactate [ 73 ]. Compatible solutes in Peptococcaceae have not been characterized; however, the theoretical energy yield, for example, in medium supplied with lactate is Δ G °′ = −160 kJ/mol, would give enough energy for the osmoprotectant synthesis or transport as, for example , Desulfovibrio vulgaris ; D. vulgaris is trophically analog to Desulfotomaculum species.…”

Section: Discussionmentioning

confidence: 99%

The Sulfate-Rich and Extreme Saline Sediment of the Ephemeral Tirez Lagoon: A Biotope for Acetoclastic Sulfate-Reducing Bacteria and Hydrogenotrophic Methanogenic Archaea

Montoya

Lozada-Chávez

Amils

et al. 2011

International Journal of Microbiology

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Our goal was to examine the composition of methanogenic archaea (MA) and sulfate-reducing (SRP) and sulfur-oxidizing (SOP) prokaryotes in the extreme athalassohaline and particularly sulfate-rich sediment of Tirez Lagoon (Spain). Thus, adenosine-5′-phosphosulfate (APS) reductase α (aprA) and methyl coenzyme M reductase α (mcrA) gene markers were amplified given that both enzymes are specific for SRP, SOP, and MA, respectively. Anaerobic populations sampled at different depths in flooded and dry seasons from the anoxic sediment were compared qualitatively via denaturing gradient gel electrophoresis (DGGE) fingerprint analysis. Phylogenetic analyses allowed the detection of SRP belonging to Desulfobacteraceae, Desulfohalobiaceae, and Peptococcaceae in ∂-proteobacteria and Firmicutes and SOP belonging to Chromatiales/Thiotrichales clade and Ectothiorhodospiraceae in γ-proteobacteria as well as MA belonging to methylotrophic species in Methanosarcinaceae and one hydrogenotrophic species in Methanomicrobiaceae. We also estimated amino acid composition, GC content, and preferential codon usage for the AprA and McrA sequences from halophiles, nonhalophiles, and Tirez phylotypes. Even though our results cannot be currently conclusive regarding the halotolerant strategies carried out by Tirez phylotypes, we discuss the possibility of a plausible “salt-in” signal in SRP and SOP as well as of a speculative complementary haloadaptation between salt-in and salt-out strategies in MA.

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Section: Discussionmentioning

confidence: 99%

The Sulfate-Rich and Extreme Saline Sediment of the Ephemeral Tirez Lagoon: A Biotope for Acetoclastic Sulfate-Reducing Bacteria and Hydrogenotrophic Methanogenic Archaea

Montoya

Lozada-Chávez

Amils

et al. 2011

International Journal of Microbiology

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“…In our experiments (up to 14-30 days), growth on acetate coupled with sulfide production was not observed. However, very slow acetate consumption by both strains after 56 days of cultivation has been noted [7]. The strains 435 T and 781 used sulfate, sulfite, and thiosulfate, but not nitrate as electron acceptors and carried out thiosulfate dismutation to sulfate and sulfide.…”

Section: Phenotypic Characteristics Of Strains 435 T and 781mentioning

confidence: 99%

“…salinus [ 4 ]. Morphology, ultrastructural organization, and physiology, including the ability to fix nitrogen of strains 435 T and 781 were studied, and the results were reported in papers [ 5 , 6 , 7 ]. DNA-DNA hybridization and phylogenetic analysis of their 16S rRNA genes were performed, which made it possible to classify strains 435 T and 781 to the new species ‘ Desulfotomaculum salinum ’ and to propose a description of the species [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

A Novel View on the Taxonomy of Sulfate-Reducing Bacterium ‘Desulfotomaculum salinum’ and a Description of a New Species Desulfofundulus salinus sp. nov.

Nazina,

Tourova,

Grouzdev

et al. 2024

Microorganisms

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Two thermophilic spore-forming sulfate-reducing strains, 435T and 781, were isolated from oil and gas reservoirs in Western Siberia (Russia) about 50 years ago. Both strains were found to be neutrophilic, chemoorganotrophic, anaerobic bacteria, growing at 45–70 °C (optimum, 55–60 °C) and with 0–4.5% (w/v) NaCl (optimum, 0.5–1% NaCl). The major fatty acids were iso-C15:0, iso-C17:0, C16:0, and C18:0. In sulfate-reducing conditions, the strains utilized H2/CO2, formate, lactate, pyruvate, malate, fumarate, succinate, methanol, ethanol, propanol, butanol, butyrate, valerate, and palmitate. In 2005, based on phenotypic characteristics and a 16S rRNA gene sequence analysis, the strains were described as ‘Desulfotomaculum salinum’ sp. nov. However, this species was not validly published because the type strain was not deposited in two culture collections. In this study, a genomic analysis of strain 435T was carried out to determine its taxonomic affiliation. The genome size of strain 435T was 2.886 Mb with a 55.1% genomic G + C content. The average nucleotide identity and digital DNA–DNA hybridization values were highest between strain 435T and members of the genus Desulfofundulus, 78.7–93.3% and 25.0–52.2%, respectively; these values were below the species delineation cut-offs (<95–96% and <70%). The cumulative phenotypic and phylogenetic data indicate that two strains represent a novel species within the genus Desulfofundulus, for which the name Desulfofundulus salinus sp. nov. is proposed. The type strain is 435T (=VKM B-1492T = DSM 23196T). A genome analysis of strain 435T revealed the genes for dissimilatory sulfate reduction, autotrophic carbon fixation via the Wood–Ljungdahl pathway, hydrogen utilization, methanol and organic acids metabolism, and sporulation, which were confirmed by cultivation studies.

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“…Sterile stock solutions of the following carbon sources: acetate, benzoate, butyrate, formate, fumarate, lactate, malate, propionate, pyruvate, succinate, fructose, glucose, ethanol, was added to medium at 10 mM final concentration as previously described. [15][16][17][18][19][20] Cell growth was identified as positive with blackening of the media in three successive subcultures. Salt requirement of isolate was determined in Medium 63a supplemented with various concentrations of NaCl.…”

Section: Physiological Studiesmentioning

confidence: 99%

Biocorrosion of Low Alloy Steel by Desulfotomaculum sp. and Effect of Biocides on Corrosion Control

2007

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Newly Discovered Properties of Spore-Forming Sulfate-Reducing Bacteria, Desulfotomaculum Strains 435 and 781

Cited by 4 publications

References 5 publications

The Sulfate-Rich and Extreme Saline Sediment of the Ephemeral Tirez Lagoon: A Biotope for Acetoclastic Sulfate-Reducing Bacteria and Hydrogenotrophic Methanogenic Archaea

The Sulfate-Rich and Extreme Saline Sediment of the Ephemeral Tirez Lagoon: A Biotope for Acetoclastic Sulfate-Reducing Bacteria and Hydrogenotrophic Methanogenic Archaea

A Novel View on the Taxonomy of Sulfate-Reducing Bacterium ‘Desulfotomaculum salinum’ and a Description of a New Species Desulfofundulus salinus sp. nov.

Biocorrosion of Low Alloy Steel by Desulfotomaculum sp. and Effect of Biocides on Corrosion Control

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