Newly developed Sr-substituted α-TCP bone cements

Pina, Sandra; Torres, Paula M.C.; Goetz-Neunhoeffer, F.; Neubauer, J.; Ferreira, J.M.F.

doi:10.1016/j.actbio.2009.09.001

Cited by 89 publications

(80 citation statements)

References 34 publications

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“…Not only is the required Sr provided at the interface with the biological medium, but also the dose of Sr delivered is said to be controlled under physiological conditions [11]. Even so, the reported biocompatibility was still limited, although all the claimed better effects were achieved, assumed to be due to the release of Sr ions [13,14]. A complication lies in the fact that the osteoblast response depends not only on the physico-chemical properties of the materials, but also the Sr content.…”

Section: Introductionmentioning

confidence: 98%

“…Recently, attention has been given to the modification of bioceramics with Sr, which has tended to enhance their biological performance, including 45S5 bioglass Ò [11], hydroxyapatite (HA) [12] and a-and b-tricalcium phosphate [13,14]. Not only is the required Sr provided at the interface with the biological medium, but also the dose of Sr delivered is said to be controlled under physiological conditions [11].…”

Section: Introductionmentioning

confidence: 99%

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Effects of strontium in modified biomaterials

et al. 2011

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Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Effects of strontium in modified biomaterials

et al. 2011

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“…[4][5][6] Furthermore, it has been found to enhance effectively the biological performance of implanted materials through partial substitution for Ca, due to chemical similarity, including 45S5 bioglass, 7 hydroxyapatite (HA), 8 and R-and β-TCP. 9,10 However, current pharmaceutical therapies focus mainly on modulating the activity of either osteoblasts or osteoclasts, or both, but pay little attention to the microenvironment or milieu where the cells reside. In fact, it is well established that the local milieu in osteoporotic bone is acidotic.…”

Section: Introductionmentioning

confidence: 99%

Interfacial pH: A Critical Factor for Osteoporotic Bone Regeneration

et al. 2011

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Osteoporosis is a disease attributed to an imbalance in communication between osteoblasts and osteoclasts, possibly arising from a locally acidic microenvironment which hinders normal cell function. However, to date, little or no attention has been paid to these cells' milieu in respect of implant materials. Although it has been claimed for a few biomaterials that they stimulate bone formation, seldom has their surface behavior been invoked to explain behavior. With degradation, ion concentrations and pH at the material's surface must vary and thus may affect osteoblast response directly. On degradation of a recently developed biomaterial, Sr-containing CaSiO3, the interfacial pH was found to be appreciably higher than that of the bulk medium and the "standard" physiological value of 7.4. At these high values (pH > 8), both the proliferation and alkaline phosphatase (ALP) activity of osteoblasts was significantly enhanced, with a maximum response at 10% Sr substitution for Ca. This shows that the chemistry of the solid-liquid interface is a critical factor in bone regeneration, although this has generally been overlooked. Thus, the interfacial pH in particular is to be considered, rather than the bulk value, and this may be of importance in many related contexts in bone-tissue engineering.

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“…Finally, Alizarin red staining was carried out to qualitatively assess the induction of osteogenic differentiation of the hASCs by the ionic-doped scaffolds. [Pina et al, 2009[Pina et al, , 2010bTorres et al, 2014]. The molar ratio of (Ca + X)/P = 1.48 (where X corresponds to Sr, Zn, or Mn) was used in order to avoid hydroxyapatite (HAp) formation (with a pH of approx.…”

Section: +mentioning

confidence: 99%

Biofunctional Ionic-Doped Calcium Phosphates: Silk Fibroin Composites for Bone Tissue Engineering Scaffolding

Pina

Canadas

Jiménez³

et al. 2017

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The treatment and regeneration of bone defects caused by traumatism or diseases have not been completely addressed by current therapies. Lately, advanced tools and technologies have been successfully developed for bone tissue regeneration. Functional scaffolding materials such as biopolymers and bioresorbable fillers have gained particular attention, owing to their ability to promote cell adhesion, proliferation, and extracellular matrix production, which promote new bone growth. Here, we present novel biofunctional scaffolds for bone regeneration composed of silk fibroin (SF) and β-tricalcium phosphate (β-TCP) and incorporating Sr, Zn, and Mn, which were successfully developed using salt-leaching followed by a freeze-drying technique. The scaffolds presented a suitable pore size, porosity, and high interconnectivity, adequate for promoting cell attachment and proliferation. The degradation behavior and compressive mechanical strengths showed that SF/ionic-doped TCP scaffolds exhibit improved characteristics for bone tissue engineering when compared with SF scaffolds alone. The in vitro bioactivity assays using a simulated body fluid showed the growth of an apatite layer. Furthermore, in vitro assays using human adipose-derived stem cells presented different effects on cell proliferation/differentiation when varying the doping agents in the biofunctional scaffolds. The incorporation of Zn into the scaffolds led to improved proliferation, while the Sr- and Mn-doped scaffolds presented higher osteogenic potential as demonstrated by DNA quantification and alkaline phosphatase activity. The combination of Sr with Zn led to an influence on cell proliferation and osteogenesis when compared with single ions. Our results indicate that biofunctional ionic-doped composite scaffolds are good candidates for further in vivo studies on bone tissue regeneration.

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Newly developed Sr-substituted α-TCP bone cements

Cited by 89 publications

References 34 publications

Effects of strontium in modified biomaterials

Effects of strontium in modified biomaterials

Interfacial pH: A Critical Factor for Osteoporotic Bone Regeneration

Biofunctional Ionic-Doped Calcium Phosphates: Silk Fibroin Composites for Bone Tissue Engineering Scaffolding

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