Newly Designed Primer Sets Available for Evaluating Various Cytokines and iNOS mRNA Expression in Guinea Pig Lung Tissues by RT-PCR

Yamada, Hiroyuki; Udagawa, Tadashi; Mizuno, Satoru; Hiramatsu, Kumiko; Sugawara, Isamu

doi:10.1538/expanim.54.163

Cited by 25 publications

(21 citation statements)

References 23 publications

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“…cDNA was produced by using an iScript cDNA synthesis kit (Bio-Rad), and quantitative real-time PCR was performed using iQ Syber green Supermix (Bio-Rad). Primers to amplify IFN-␥, TNF-␣, IL-12, IL-1␤, and ␤-actin for real-time PCR were obtained from previously published sequences (2,7,29,43). The primer sequences for IL-10 were forward, TTCTTCCAAACAC AGGATCAGC, and reverse, TCATTTCCGATAGGGCTTGG.…”

Section: Mycobacterium Speciesmentioning

confidence: 99%

Kinetics of the Immune Response Profile in Guinea Pigs after Vaccination withMycobacterium bovisBCG and Infection withMycobacterium tuberculosis

et al. 2009

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Section: Mycobacterium Speciesmentioning

confidence: 99%

Kinetics of the Immune Response Profile in Guinea Pigs after Vaccination withMycobacterium bovisBCG and Infection withMycobacterium tuberculosis

et al. 2009

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“…More recently, inflammation/ infection related microarrays have been developed and validated to some extent in guinea pigs. Immunohistochemical and flow cytometric analyses are also possible in guinea pigs and siRNA gene silencing and adenoviral gene transfection have been used in guinea pigs [295][296][297][298][299][300][301][302][303].…”

Section: Geneticsmentioning

confidence: 99%

Using guinea pigs in studies relevant to asthma and COPD

Canning

Chou

2008

Pulmonary Pharmacology & Therapeutics

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The guinea pig has been the most commonly used small animal species in preclinical studies related to asthma and COPD. The primary advantages of the guinea pig are the similar potencies and efficacies of agonists and antagonists in human and guinea pig airways and the many similarities in physiological processes, especially airway autonomic control and the response to allergen. The primary disadvantages to using guinea pigs are the lack of transgenic methods, limited numbers of guinea pig strains for comparative studies and a prominent axon reflex that is unlikely to be present in human airways. These attributes and various models developed in guinea pigs are discussed.

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“…These samples were snap-frozen in liquid nitrogen and stored at −85˚C until use. RNA extraction was performed as described previously (Yamada et al 2005). Briefly, the frozen tissues were homogenized in a microcentrifuge tube with an autoclaved disposable 1000-μ L tip cooled by dipping in liquid nitrogen.…”

Section: Real-time Pcrmentioning

confidence: 99%

Higher Susceptibility of Type 1 Diabetic Rats to Mycobacterium tuberculosis Infection

Sugawara

Mizuno

2008

Tohoku J. Exp. Med.

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An association between diabetes mellitus and tuberculosis has been implicated for a long time. We have previously reported that Goto Kakizaki type 2 diabetic rats are highly susceptible to Mycobacterium (M.) tuberculosis infection. As a next step, we attempted to clarify whether type 1 diabetic rats are more susceptible to M. tuberculosis than nondiabetic wild-type (WT) rats. Here, we used the Komeda diabetes-prone (KDP) rat, as a model of type 1 diabetes mellitus. The infected KDP rats developed large granulomas without central necrosis in their lungs, liver or spleen. This was consistent with a significant increase in the number of colony-forming units (cfu) of M. tuberculosis in the lungs and spleen ( p < 0.01). Insulin treatment resulted in significant reduction of tubercle bacilli in the infected KDP rats ( p < 0.01). Pulmonary levels of interferon-γ , tumor necrosis factor-α and interleukin-1β mRNAs were higher in the infected diabetic rats than in WT rats. Alveolar macrophages from KDP rats were not fully activated by M. tuberculosis infection because the macrophages did not secrete nitric oxide (NO) that can kill M. tuberculosis ( p < 0.01), but no significant difference in phagocytosis of tubercle bacilli by alveolar macrophages was observed between KDP and WT rats. Taken together, our findings indicate that type 1 diabetic rats are more susceptible to M. tuberculosis that WT rats. type 1 diabetic rat; type 1 diabetes mellitus; tuberculosis; cytokine.Tohoku

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Newly Designed Primer Sets Available for Evaluating Various Cytokines and iNOS mRNA Expression in Guinea Pig Lung Tissues by RT-PCR

Abstract: Guinea pigs are often used as an animal model of human tuberculosis (TB

Cited by 25 publications

References 23 publications

Kinetics of the Immune Response Profile in Guinea Pigs after Vaccination withMycobacterium bovisBCG and Infection withMycobacterium tuberculosis

Kinetics of the Immune Response Profile in Guinea Pigs after Vaccination withMycobacterium bovisBCG and Infection withMycobacterium tuberculosis

Using guinea pigs in studies relevant to asthma and COPD

Higher Susceptibility of Type 1 Diabetic Rats to Mycobacterium tuberculosis Infection

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