Newcastle Disease Virus-Based Live Attenuated Vaccine Completely Protects Chickens and Mice from Lethal Challenge of Homologous and Heterologous H5N1 Avian Influenza Viruses

Ge, Jinying; Deng, Guohua; Wen, Zhiyuan; Tian, Guobing; Wang, Yong; Shi, Jianzhong; Wang, Xijun; Li, Yanbing; Hu, Sen; Jiang, Yongping; Yang, Chinglai; Yu, Kangzhen; Bu, Zhigao; Chen, Hualan

doi:10.1128/jvi.01514-06

Cited by 246 publications

(210 citation statements)

References 37 publications

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“…There are many reports on effectiveness of using live recombinant vaccines based on chicken pox and Newcastle disease viruses with expressed gene of epizootically actual AIV strains in prophylaxis of the highly pathogenic avian influenza A (H5N1) (Qiao et al, 2003, Veits et al, 2006, Ge et al, 2007. The most important advantage of the live recombinant vaccines is their ability to rapidly form adequate immunity (humoral and cellular) as soon as on the 7 th day after single vaccination.…”

Section: Discussionmentioning

confidence: 99%

Immunogenicity of the inactivated oil emulsion influenza A (H5N1) vaccine in chickens

Kydyrbayev¹,

Tabynov²,

Ryskeldynova³

et al. 2010

ABJNA

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The goal of the presented research was to evaluate immunogenicity of the inactivated emulsified avian influenza (AI) type A (H5N1) vaccine that was developed on the basis of epizootically important autogenic AI virus strain A/domestic goose/Pavlodar/1/05 (H5N1) isolated in the epizooty focus in Pavlodarskaya oblast, Republic of Kazakhstan, in 2005. The results of the performed study show high level of vaccine immunogenicity. The vaccine is able to form the immunity for 12 months in 2-months chickens immunized in dose of 0.5 ml. The antihemagglutinating antibodies were detected in 14 days and reached their peak of 1:277 on the 6 th week post vaccination. The vaccination effectiveness is 100% from the 28 th day till the 150 th day post vaccination, then slowly decreases down to the level of 80% till day 360 post immunization (period of observation) that is sufficient for ensuring the safety of the vaccinated birds. Moreover the vaccine has high level of protectiveness demonstrating 100% protection of vaccinated birds on the 28 th day post vaccination and preventing virus shedding from cloaks and tracheas of chickens challenged with a field strain. The results of the study allow concluding that the developed inactivated emulsified AI vaccine of subtype H5 on the basis of the A/domestic goose/Pavlodar/1/05 (H5N1) possesses high protective ability and long-term immunogenicity.

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Section: Discussionmentioning

confidence: 99%

Immunogenicity of the inactivated oil emulsion influenza A (H5N1) vaccine in chickens

Kydyrbayev¹,

Tabynov²,

Ryskeldynova³

et al. 2010

ABJNA

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“…Virus ND kadang-kadang bersama dengan AIV menginfeksi unggas dan memiliki kesamaan yang signifikan dengan AIV dalam menyebabkan kematian pada unggas di industri perunggasan (Ge et al, 2007). Gejala klinis NDV pada unggas berupa gejala syaraf yang menonjol yang menunjukkan, ataksia, hilang keseimbangan, kepala berputar-putar tidak beraturan (tortikolis) ataupun rebah di atas punggung, paralisis kaki (Alexander and Senne, 2008;Cattoli et al, 2011).…”

Section: Avian Influenza Virus (Aiv) Danunclassified

Pendekatan Diagnosis Avian Influenza Virus dan Newcastle Disease Virus pada Kasus Lapangan Ayam Petelur: Imunopatologis Streptavidin Biotin

Ekaningtias

Wuryastuty

Wasito

2017

Jurnal Sain Veteriner

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Poultry can be infected not only by one type of virus, but also by more than one type of virus. Avian influenza virus (AIV) and Newcastle disease virus (NDV) are two important pathogens in poultry that can cause recurrent outbreaks and often lead to clinical symptoms and pathological lesions similar in poultry with high morbidity and mortality causing significant economic losses to the poultry industry. This study aims to detect and differentiate pathogenic viruses in commercial laying chickens with similar clinical symptoms, especially torticolis and curled toe paralysis, and anatomic pathological lesions, such as foci necrotic hemorrhages in the digestive tract by applying streptavidin-biotin immunohistochemical immunological test (IHC SB).Samples (chickens) were taken from the cases of the disease in poultry in several commercial poultry farms. The layer chickens in which showed clinical symptoms of torticolis and curled toe paralysis, and hemorrhagic lesions in the lungs and / or digestive system are suspected of having AIV and NDV infections. After being necropsied, then lungs and gastrointestinal tract were tested IHC SB. The IHC SB results were examined with a digital microscope camera system. The results of the present study indicated that AIV antigen was detected in the lungs, but not in the digestive tract, whereas, NDV was detected in the digestive tract, but not in the lungs.It was concluded, that the IHC SB can be applied for confirmation of the diagnosis of AIV and NDV and indicated, that the commercial laying chickens with clinical symptoms of torticolis and curled toe paralysis, as well as pathological hemorrhagic lesions in the gastrointestinal tract could be infected by both viruses. Keywords: AIV, NDV, IHC SB, torticolis, paralysis AbstrakUnggas tidak hanya dapat terinfeksi oleh satu jenis virus, tetapi dapat juga terinfeksi oleh lebih dari satu jenis virus. Virus avian influenza dan Newcastle disease adalah dua patogen penting pada unggas yang dapat menyebabkan wabah berulang dan seringkali menimbulkan gejala klinis dan lesi patologis yang serupa pada unggas dengan morbiditas dan mortalitas tinggi sehingga menyebabkan kerugian ekonomis yang besar pada industri perunggasan. Penelitian ini bertujuan untuk mendeteksi AIV dan/atau NDV pada ayam petelur komersial dengan gejala klinis serupa, terutama tortikolis dan paralisis kaki serta lesi patologis anatomis berupa foki nekrotik hemoragis pada saluran pencernaan dengan uji imunologis imunohistokimia streptavidin biotin (IHK SB). Sampel diambil dari kasus-kasus penyakit pada unggas di peternakan ayam petelur komersial. Ayam petelur tersebut menunjukkan gejala klinis tortikolis dan paralisis kaki, serta lesi hemoragis pada paru-paru dan/atau sistem pencernaan yang diduga terinfeksi AIV dan NDV. Setelah unggas dinekropsi, paru-paru dan saluran pencernaan diuji IHK SB kemudian diamati di bawah microscope digital camera system. Hasil penelitian ini membuktikan, bahwa dengan pewarnaan IHK SB, antigen AIV dapat dideteksi pada paru-paru, tetapi t...

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“…Among candidates, fowl pox virus (FPV), Newcastle disease virus (NDV), infectious laryngotracheitis virus (ILTV), Marek disease virus (MDV-1) and herpesvirus of turkeys (HVT) are receiving the most attention as potential vectors, since these viruses have restricted host ranges among avian species. 14,[16][17][18][19] The recombinant FP vector-based vaccine "Trovac AI H5" was licensed in the USA in 1998 for the immunization of poultry and an NDV-based live attenuated vaccine "NDV-H5HA" is commercially available in China. 20 However, there are some inherent shortcomings, such as lower susceptibility in very young birds due to maternally derived antibodies, that limit their effectiveness and commercial use.…”

Section: Engineering Hvt As a Bivalent Vaccine For Avian Influenza Anmentioning

confidence: 99%

Progress toward the development of polyvalent vaccination strategies against multiple viral infections in chickens using herpesvirus of turkeys as vector

Iqbal

2012

biobugs

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Newcastle Disease Virus-Based Live Attenuated Vaccine Completely Protects Chickens and Mice from Lethal Challenge of Homologous and Heterologous H5N1 Avian Influenza Viruses

Abstract: H5N1 highly pathogenic avian influenza virus (HPAIV

Cited by 246 publications

References 37 publications

Immunogenicity of the inactivated oil emulsion influenza A (H5N1) vaccine in chickens

Immunogenicity of the inactivated oil emulsion influenza A (H5N1) vaccine in chickens

Pendekatan Diagnosis Avian Influenza Virus dan Newcastle Disease Virus pada Kasus Lapangan Ayam Petelur: Imunopatologis Streptavidin Biotin

Progress toward the development of polyvalent vaccination strategies against multiple viral infections in chickens using herpesvirus of turkeys as vector

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