Newborn pig skin as model membrane in in vitro drug permeation studies: A technical note

Cilurzo, Francesco; Minghetti, Paola; Sinico, Chiara

doi:10.1208/pt0804094

Cited by 77 publications

(53 citation statements)

References 9 publications

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“…Newborn pig skin has attracted considerable attention for evaluation of topical drug formulations (Cilurzo et al, 2007). However, the diversity in the thickness of newborn pig skin regarding the age of animal ranging from only one day old pig (1.2 kg) (Manconi et al, 2011b;Mura et al, 2009) to 40 days old animal (20 kg) needs to be taken into the consideration (Wang et al, 2014).…”

Section: Porcine Skinmentioning

confidence: 99%

In vitro skin models as a tool in optimization of drug formulation

Flaten

Palac²,

Engesland

et al. 2015

European Journal of Pharmaceutical Sciences

172

135

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Abstract(Trans)dermal drug therapy is gaining increasing importance in the modern drug development. To fully utilize the potential of this route, it is important to optimize the delivery of active ingredient/drug into/through the skin. The optimal carrier/vehicle can enhance the desired outcome of the therapy therefore the optimization of skin formulations is often included in the early stages of the product development. A rational approach in designing and optimizing skin formulations requires well-defined skin models, able to identify and evaluate the intrinsic properties of the formulation. Most of the current optimization relies on the use of suitable ex vivo animal/human models.However, increasing restrictions in use and handling of animals and human skin stimulated the search for suitable artificial skin models. This review attempts to provide an unbiased overview of the most commonly used models, with emphasis on their limitations and advantages. The choice of the most applicable in vitro model for the particular purpose should be based on the interplay between the availability, easiness of the use, cost and the respective limitations.

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Section: Porcine Skinmentioning

confidence: 99%

In vitro skin models as a tool in optimization of drug formulation

Flaten

Palac²,

Engesland

et al. 2015

European Journal of Pharmaceutical Sciences

172

135

View full text Add to dashboard Cite

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“…Porcine ear skins were used as the in vitro model for this study, because its essential permeation characteristics were similar with human skin [26,27] . The percutaneous absorption experiment of the four creams was performed through a drug transdermal diffusion absorption apparatus (Huanghai, model RYJ-6A, China).…”

Section: Transdermal Permeation Experimentmentioning

confidence: 99%

Preparation and Characterization of Microcapsules Encapsulating Octyl Methoxycinnamate by Complex Coacervation

Zhao¹

2016

ijps

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Zhao, et al.,: Octyl Methoxycinnamate-loaded MicrocapsulesThe aim of this study was to entrap the lipophilic ultraviolet absorber, octyl methoxycinnamate in microcapsuels to enhance its application. A complex coacervation method was established. Through the appearance of encapsulated glass beads prepared with different wall materials, gelatin and sodium polyphosphate were selected as wall materials because of dense structure and less adhesion. The effects of three variables, core/ (core+wall) ratio, pH at complex coacervation, and amount of cross-linking agent, on encapsulation efficiency were studied. Response surface methodology was employed to optimize the technology, and a polynomial regression model equation was generated. The optimization method enabled to predict the response variable value within the experimental range with good agreement between the predicted and experimental values. The microcapsules under the optimized conditions appeared spherical in shape with smooth nonporous surface and no tendency to aggregation. The photodegradation percentage of octyl methoxycinnamate after exposing to ultraviolet irradiation was determined and the transdermal permeation pattern of octyl methoxycinnamate and its photodegradation product from various formulations was estimated in vitro. From the results obtained, the microcapsules had achieved decreasing transdermal permeation of octyl methoxycinnamate and its photodegradation product, enhancing the accumulation of the sunscreen at the administration site, and then increasing the protection effect and safety. This study revealed the potential of sunscreen microcapsules as new skin drug delivery systems.

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“…The pigskin was used for the permeation study because the pig stratum corneum is the most similar to human stratum corneum in terms of lipid composition (26). The permeation studies of freshly excised pigskin were performed in triplicate (at least three times in order to achieve statistical significance) by means of vertical Franz diffusion cells with an effective diffusion area of 1.53 cm 2 .…”

Section: Permeation Of Resveratrol Through the Pigskin Ex Vivomentioning

confidence: 99%

Cyclodextrin-Based Nanosponges for Delivery of Resveratrol: In Vitro Characterisation, Stability, Cytotoxicity and Permeation Study

et al. 2011

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Abstract. The aim of this work was to increase the solubility, stability and permeation of resveratrol by complexation with cyclodextrin-based nanosponges (NS). Nanosponges are recently developed hypercross-linked cyclodextrin polymers nanostructured to form three-dimensional networks; they are obtained by reacting cyclodextrin with a cross-linker such as carbonyldiimidazole. They have been used to increase the solubility and stability of poorly soluble actives. This study aimed at formulating complexes of resveratrol with β-cyclodextrin nanosponges in different weight ratios. DSC, FTIR and Xray powder diffraction (XRPD) studies confirmed the interaction of resveratrol with NS. XRPD showed that the crystallinity of resveratrol decrease after encapsulation. The particle sizes of resveratrol-loaded NS are in between 400 to 500 nm with low polydispersity indices. Zeta potential is sufficiently high to obtain a stable colloidal nanosuspension. TEM measurement also revealed a particle size around 400 nm for NS complexes. The in vitro release and stability of resveratrol complex were increased compared with plain drug. Cytotoxic studies on HCPC-I cell showed that resveratrol formulations were more cytotoxic than plain resveratrol. The permeation study indicates that the resveratrol NS formulation showed good permeation in pigskin. The accumulation study in rabbit mucosa showed better accumulation of resveratrol NS formulation than plain drug. These results signify that resveratrol NS formulation can be used for buccal delivery and topical application.

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Newborn pig skin as model membrane in in vitro drug permeation studies: A technical note

Cited by 77 publications

References 9 publications

In vitro skin models as a tool in optimization of drug formulation

In vitro skin models as a tool in optimization of drug formulation

Preparation and Characterization of Microcapsules Encapsulating Octyl Methoxycinnamate by Complex Coacervation

Cyclodextrin-Based Nanosponges for Delivery of Resveratrol: In Vitro Characterisation, Stability, Cytotoxicity and Permeation Study

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