New yeast expression platforms based on methylotrophic and and on dimorphic and – A comparison

Abstract

Yeasts combine the ease of genetic manipulation and fermentation of a microbial organism with the capability to secrete and to modify proteins according to a general eukaryotic scheme. Yeasts thus provide attractive platforms for the production of recombinant proteins. Here, four important species are presented and compared: the methylotrophic Hansenula polymorpha and Pichia pastoris, distinguished by an increasingly large track record as industrial platforms, and the dimorphic species Arxula adeninivorans and Yarrrowia lipolytica, not yet established as industrial platforms, but demonstrating promising technological potential, as discussed in this article.

Synthesis and Accumulation of Cyanophycin in Transgenic Strains of Saccharomyces cerevisiae

“…A chromosomal integration of cphA in yeast would probably be especially advantageous, thereby allowing the use of technical, low-cost media instead of specific minimal media. In addition, the use of other yeasts such as P. pastoris and H. polymorpha (17), which have become increasingly interesting for biotechnical purposes because they exhibit high levels of productivity and because they can be grown to high cell densities, could be applied in the future.…”

“…Yeast-based expression systems excel because of their available constitutive or strongly inducible promoters and their growth to high cell densities on inexpensive substrates. The range of today's established yeast expression systems includes S. cerevisiae, Kluyveromyces lactis, Pichia pastoris, Yarrowia lipolytica, Arxula adeninivorans, and Hansenula polymorpha (8,17). Polymers such as human collagen and recombinant gelatin have also been produced successfully in several yeast strains (7,…”

Uricase production by a recombinant Hansenula polymorpha strain harboring Candida utilis uricase gene

“…sp, and recombinant uricase from E. coli or S. cerevisiae are now commercially available, but the production procedures still have the problems with low productivity, complicated isolation, and purification procedure for clinical applications (Li et al 2006;Lotfy 2008). The methylotrophic yeast H. polymorpha has been proven to be an effective expression system for the production of heterologous proteins with medical application (Gellissen 2000;Gellissen et al 2005). In this study, the C. utilis uricase was successfully expressed in H. polymorpha for the first time.…”

“…The expression of the key methanolmetabolizing enzymes, such as methanol oxidase (MOX; Ledeboer et al 1985) and formate dehydrogenase (Gellissen et al 1996), can amount up to one third of the total cellular protein during growth on methanol. The existence of inducible strong promoters, the formation of integrants in high copy numbers, high cell density (>100 g dry weight per liter) in cheap defined media, and the low antigenicity of proteins produced in H. polymorpha make H. polymorpha a highly competitive system for the production of recombinant proteins for medical application (Gellissen 2000;Gellissen et al 2005). Several eukaryotic proteins with clinical and therapeutic values, such as hirudin, hepatitis B surface B antigen, aprotinin, and human urokinase, have been expressed in H. polymorpha (Gellissen 2000).…”

Synthetic Biology and Metabolic Engineering Approaches and Its Impact on Non-Conventional Yeast and Biofuel Production

“…Each of these organisms presents with different advantages, similarities, and differences when compared with S. cerevisiae. Most of the nonconventional yeast, like K. lactis, P. pastoris, etc., possess a broad range of substrates which is superior to S. cerevisiae and reduce the cost of industrial biofuel production (Gellissen et al, 2005). Y. lipolytica and K. lactis secrete high titers of secretory proteins extracellularly which is better than the model yeast S. cerevisiae (Dominguez et al, 1998).…”