New Water Soluble Polysaccharide From Periploca Laevigata: Functional Characterization and Evaluation of Multi-biological Activities.

Abstract

The water-soluble polysaccharide was isolated from Periploca laevigata root barks and purified. The structurale, surface, functional properties, and biological activities of P. laevigata polysaccharide (PLP1) were investigated. Scanning electron microscopy (SEM), X-ray diffraction crystallography (XRD), were used for structurale characterization of PLP1. SEM analysis suggested that the polysaccharide had irregular particle size with a large number of cavities, mostly seen as aggregates, and fibrous in nature. The samples had peaks at approximately 22°, and 38° 2θ degrees in the XRD pattern, which indicated both crystalline and amorphous structure. Functional properties of PLP1 were investigated based on water and oil holding capacity, solubility, emulsification activity, and foaming ability. Interestingly, PLP1 exhibited, a noticeable ability to emulsify corn and olive oil. Experimental analysis indicated that PLP1 able to decrease surface tension of water of 67.5 mN/m to 43.7 mN/m. On other trend, bioactivities evidenced that PLP1 is a potent protease and lipase inhibitor. The cell viability proved the cytoprotective effect of PLP1 on cell treated with hydrogen peroxide H2O2, suppressing apoptosis and ameliorating oxidative lesions. The experimental data and multi functionality provides enough evidence and open many new possibilities for biomedicine and food applications.

Recent advances in the extraction, purification, structural-property correlations, and antiobesity mechanism of traditional Chinese medicine-derived polysaccharides: a review

“…For example, Wu et al (22) assessed the anti-pancreatic lipase activity in vitro about four polysaccharide fractions (DFP-H, DFP-M, DFP-U, and DFP-P) obtained from D. indusiate through different extraction techniques, as well as the inhibition of PL has been determined as 58.39 ± 0.98%, 52.65 ± 0.83%, 54.67 ± 1.03%, and 63.20 ± 1.11%, correspondingly. Other researches (103)(104)(105)(106)(107) also demonstrated that TCMPOs exhibit obvious inhibitory effects on PL, which indicates that TCMPOs could be developed as potential antiobesity agents to impede the digestion and absorption of dietary fat.…”

“…Moreover, Shi et al ( 111 ) observed that Ophiopogon japonicus polysaccharides (MDG-1) could reduce the synthesis of cholesterol in the liver and inhibit body growth within obese mice by binding to BAs in the lumen, reducing their reflux into the liver, and suppressing the enterohepatic circulation of BAs. In addition to the two principal targets of preventing fat digestion and absorption demonstrated above, the polysaccharides extracted from some kinds of TCM could also affect how well exogenous lipids are absorbed and digested by decreasing the activity of α-amylase, pepsin and trypsin ( 103 , 104 , 106 , 107 ), affecting the stability of the cholesterol-micelle and reducing the amount of the unbroken cholesterol-micelle ( 22 , 103 , 106 ).…”