New volumetric CNT-doped gelatin–cellulose scaffolds for skeletal muscle tissue engineering

Velasco-Mallorquí, Ferran; Fernández‐Costa, Juan M.; Neves, Luísa A.; Ramón‐Azcón, Javier

doi:10.1039/d0na00268b

Cited by 32 publications

(19 citation statements)

References 69 publications

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“…Several studies conclude that electrical stimulation and mechanical tension favor the maturation of the tissues. 35 , 61 , 79 Current studies to develop human functional bioartificial muscle use fibrin-based hydrogels. Fibrin composite matrices have tunable mechanical properties, can be remodeled by cells, and allow long-term cultures.…”

Section: Discussionmentioning

confidence: 99%

“…For this reason, anisotropic gelatin-cellulose cryogels were generated to engineer volumetric skeletal muscle using C2C12 myoblasts. 61 Anisotropic cryogels improve cell alignment, myotube fusion, and myogenic maturation ( Figure 4(c) ). Moreover, the addition of carbon nanotubes (CNTs) to the cryogel improves the electrical properties of the scaffolds, which enhances early myogenic maturation steps when electric pulse stimulation is applied.…”

Section: D Engineering For Skeletal Muscle Culturementioning

confidence: 99%

“…In order to mimic the extracellular environment and the native cellular morphology, the main bioengineering strategy is focused on the 3D encapsulation of muscular cell precursors in biocompatible materials. In the last years, 3D bioprinting, 43 – 54 hydrogel molding, 55 – 59 and microporous scaffolds 60 – 62 have been implemented to fabricate skeletal muscle tissues.…”

Section: D Engineering For Skeletal Muscle Culturementioning

confidence: 99%

“…Scale bars: 100 μm. Adapted from Velasco-Mallorqui et al 61 . The images in panel (a) are not published under the terms of the CC-BY license of this article.…”

Section: D Engineering For Skeletal Muscle Culturementioning

confidence: 99%

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Bioengineeredin vitroskeletal muscles as new tools for muscular dystrophies preclinical studies

et al. 2021

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Muscular dystrophies are a group of highly disabling disorders that share degenerative muscle weakness and wasting as common symptoms. To date, there is not an effective cure for these diseases. In the last years, bioengineered tissues have emerged as powerful tools for preclinical studies. In this review, we summarize the recent technological advances in skeletal muscle tissue engineering. We identify several ground-breaking techniques to fabricate in vitro bioartificial muscles. Accumulating evidence shows that scaffold-based tissue engineering provides topographical cues that enhance the viability and maturation of skeletal muscle. Functional bioartificial muscles have been developed using human myoblasts. These tissues accurately responded to electrical and biological stimulation. Moreover, advanced drug screening tools can be fabricated integrating these tissues in electrical stimulation platforms. However, more work introducing patient-derived cells and integrating these tissues in microdevices is needed to promote the clinical translation of bioengineered skeletal muscle as preclinical tools for muscular dystrophies.

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Section: Discussionmentioning

confidence: 99%

Section: D Engineering For Skeletal Muscle Culturementioning

confidence: 99%

Section: D Engineering For Skeletal Muscle Culturementioning

confidence: 99%

“…Scale bars: 100 μm. Adapted from Velasco-Mallorqui et al 61 . The images in panel (a) are not published under the terms of the CC-BY license of this article.…”

Section: D Engineering For Skeletal Muscle Culturementioning

confidence: 99%

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Bioengineeredin vitroskeletal muscles as new tools for muscular dystrophies preclinical studies

et al. 2021

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“…They reported that GO-implemented aligned nanofibers did not exhibit the same cell orientation as the cells in contact with pure PCL-aligned nanofibers. Carbon nanotubes (CNTs) have been investigated for the formulation of scaffolds for the potential application of skeletal muscle regeneration [ 181 ]. Patel et al built CNT-based hierarchical scaffolds in two different forms, interconnected microporous foams or aligned fiber mats, with tunable physicochemical behavior.…”

Section: Biomaterials For the Transplantation Of Striated Muscle Cellsmentioning

confidence: 99%

Current Strategies for the Regeneration of Skeletal Muscle Tissue

Alarçin

Bal‐Öztürk

Avcı

et al. 2021

IJMS

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Traumatic injuries, tumor resections, and degenerative diseases can damage skeletal muscle and lead to functional impairment and severe disability. Skeletal muscle regeneration is a complex process that depends on various cell types, signaling molecules, architectural cues, and physicochemical properties to be successful. To promote muscle repair and regeneration, various strategies for skeletal muscle tissue engineering have been developed in the last decades. However, there is still a high demand for the development of new methods and materials that promote skeletal muscle repair and functional regeneration to bring approaches closer to therapies in the clinic that structurally and functionally repair muscle. The combination of stem cells, biomaterials, and biomolecules is used to induce skeletal muscle regeneration. In this review, we provide an overview of different cell types used to treat skeletal muscle injury, highlight current strategies in biomaterial-based approaches, the importance of topography for the successful creation of functional striated muscle fibers, and discuss novel methods for muscle regeneration and challenges for their future clinical implementation.

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Sustainable Nanobiocomposites

Shah

Patel

Chavda

et al. 2022

Sustainable Nanotechnology

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New volumetric CNT-doped gelatin–cellulose scaffolds for skeletal muscle tissue engineering

Abstract: Currently, the fabrication of scaffolds for engineered skeletal muscle tissues is unable to reach the millimeter size.

Cited by 32 publications

References 69 publications

Bioengineeredin vitroskeletal muscles as new tools for muscular dystrophies preclinical studies

Bioengineeredin vitroskeletal muscles as new tools for muscular dystrophies preclinical studies

Current Strategies for the Regeneration of Skeletal Muscle Tissue

Sustainable Nanobiocomposites

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