New visible and selective DNA staining method in gels with tetrazolium salts

Paredes, Aaron J.; Naranjo-Palma, Tatiana; Alfaro-Valdés, Hilda M.; Barriga, Andrés; Babul, Jorge; Wilson, Christian A.M.

doi:10.1016/j.ab.2016.11.004

Cited by 5 publications

(4 citation statements)

References 16 publications

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“…Tetrazolium violet (TZV) having an electron-withdrawing chemical group, such as nitroblue tetrazolium, has been shown to bind its electrons using its positive tetrazole ring. 24 TZV is an important pharmaceutical intermediate for the preparation of various medicines. High concentrations of TZV can conduce to total inhibition of bacterial growth and TZV have been widely used for microbiological studies.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, exploiting the high-efficiency and nontoxic inhibitors for copper corrosion in acid solutions is of great importance. Tetrazolium violet (TZV) having an electron-withdrawing chemical group, such as nitroblue tetrazolium, has been shown to bind its electrons using its positive tetrazole ring . TZV is an important pharmaceutical intermediate for the preparation of various medicines.…”

Section: Introductionmentioning

confidence: 99%

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Electrochemical Investigation of Tetrazolium Violet as a Novel Copper Corrosion Inhibitor in an Acid Environment

Tao

Liu

et al. 2020

ACS Omega

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Tetrazolium violet (TZV) is an important pharmaceutical intermediate for the preparation of various medicines, taking into account microbiological studies and TZV as a new inhibitor of heterocyclic compound. The corrosion inhibiting action of TZV for copper in 0.5 M H2SO4 solutions was assessed by potentiodynamic polarization and electrochemical impedance spectroscopy. The corroded copper surfaces were evaluated by scanning electron microscopy. Thereafter, the thermodynamics of TZV adsorption onto copper was computed and evaluated. As a result, the target TZV compound shows great corrosion inhibition performance to protect copper in sulfuric acid. Thermodynamic test results reveal that the Langmuir, Dhar–Flory–Huggins, and Bockris–Swinkels isotherm models provide a better description for the adsorption process of TZV on the metal surface. The calculated values of ΔG ads 0 indicate a spontaneous adsorption process of TZV on the copper surface accompanied by two kinds of interactions, physical adsorption and chemisorption.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Electrochemical Investigation of Tetrazolium Violet as a Novel Copper Corrosion Inhibitor in an Acid Environment

Tao

Liu

et al. 2020

ACS Omega

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“…(1,3,4,8,9) It is desirable to use a blue light transilluminator if possible, since UV light is not only harmful to the experimenter but also causes DNA damage during analysis. (4,(10)(11)(12)(13)(14) Generally, a gel document system is usually large because it includes UV fluorescence tubes, a darkroom, a high-performance camera for image capture, and a personal computer for camera control. (15) Even with the blue transilluminator, there is still room for further reduction in size because of the height required to achieve uniform light.…”

Section: Introductionmentioning

confidence: 99%

Low-cost Miniaturization of Gel Document System Using Blue LED

Koo¹,

Jo²,

Park³

et al. 2019

Sensors and Materials

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In this paper, we propose an inexpensive and compact gel document system in an Internet-of-Things (IoT) form employing recent open platforms. The proposed system adopts a smartphone camera developed for an open platform and uses blue LEDs as the lighting device with a recently commercialized light diffuser. The camera is controlled with the open IoT platform to implement the system in the IoT form. Employing open platforms greatly reduces the cost and size of the gel document system. The light diffusers are experimentally compared and the diffuser that made the proposed system more compact is selected. The proposed system has good characteristics of the IoT system such as low price, small installation space, and Internet controllability.

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“…In a recent publication 13 , a new visible technique to stain and quantify DNA in polyacrylamide gels was proposed, using the reduction of a bivalent tetrazolium salt called nitro blue tetrazolium (NBT) and fluorescent dyes like ethidium bromide, GelRed, SYBR Green I and SYBR Gold. This reaction worked in the presence of blue light or sunlight and allowed sample recovery with improved quality compared to the use of SG I with a UV transilluminator.The objective of this paper is to provide a detailed protocol of the staining technique using tetrazolium salts.…”

Section: Introductionmentioning

confidence: 99%

DNA Staining Method Based on Formazan Precipitation Induced by Blue Light Exposure

Paredes¹,

Alfaro-Valdés²,

Wilson³

2018

JoVE

Self Cite

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DNA staining methods are very important for biomedical research. We designed a simple method that allows DNA visualization to the naked eye by the formation of a colored precipitate. It works by soaking the acrylamide or agarose DNA gel in a solution of 1x (equivalent to 2.0 µM) SYBR Green I (SG I) and 0.20 mM nitro blue tetrazolium that produces a purple precipitate of formazan when exposed to sunlight or specifically blue light. Also, DNA recovery tests were performed using an ampicillin resistant plasmid in an agarose gel stained with our method. A larger number of colonies was obtained with our method than with traditional staining using SG I with ultraviolet illumination. The described method is fast, specific, and non-toxic for DNA detection, allowing visualization of biomolecules to the "naked eye" without a transilluminator, and is inexpensive and appropriate for field use. For these reasons, our new DNA staining method has potential benefits to both research and industry.

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New visible and selective DNA staining method in gels with tetrazolium salts

Cited by 5 publications

References 16 publications

Electrochemical Investigation of Tetrazolium Violet as a Novel Copper Corrosion Inhibitor in an Acid Environment

Electrochemical Investigation of Tetrazolium Violet as a Novel Copper Corrosion Inhibitor in an Acid Environment

Low-cost Miniaturization of Gel Document System Using Blue LED

DNA Staining Method Based on Formazan Precipitation Induced by Blue Light Exposure

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