New viral vector for efficient production of target proteins in plants

Komarova, Tatiana V.; Скулачев, М. В.; Zvereva, Anna S.; Schwartz, Anton M.; Dorokhov, Yu. L.; Atabekov, J.G.

doi:10.1134/s0006297906080049

Cited by 39 publications

(41 citation statements)

References 20 publications

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“…For example, through replacement of the CP gene and the triple gene block (TGB) that encodes movement proteins, efficient production of the gene insert can be achieved. 456 Suppression of PTGS helped to improve yields, with transient co-expression of P19 from TBSV or HC-Pro from tobacco etch virus (TEV) resulting in a 44% and 83% increase in gene expression, respectively. 457 Amplicon-plus Targeting Technology utilizes this benefit of gene silencing suppression to enhance production.…”

Section: Applications Of Virus-based Particlesmentioning

confidence: 99%

Design of virus-based nanomaterials for medicine, biotechnology, and energy

2016

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Virus-based nanomaterials are versatile materials that naturally self-assemble and have relevance for a broad range of applications including medicine, biotechnology, and energy. This review provides an overview of recent developments in “chemical virology.” Viruses, as materials, provide unique nanoscale scaffolds that have relevance in chemical biology and nanotechnology, with diverse areas of applications. Some fundamental advantages of viruses, compared to synthetically programmed materials, include the highly precise spatial arrangement of their subunits into a diverse array of shapes and sizes and many available avenues for easy and reproducible modification. Here, we will first survey the broad distribution of viruses and various methods for producing virus-based nanoparticles, as well as engineering principles used to impart new functionalities. We will then examine the broad range of applications and implications of virus-based materials, focusing on the medical, biotechnology, and energy sectors. We anticipate that this field will continue to evolve and grow, with exciting new possibilities stemming from advancements in the rational design of virus-based nanomaterials.

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Section: Applications Of Virus-based Particlesmentioning

confidence: 99%

Design of virus-based nanomaterials for medicine, biotechnology, and energy

2016

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“…This new expression strategy, called magnifection ( Figure 2), combines the advantages of three biological systems: the speed and expression level/yield of the virus, the transfection efficiency of Agrobacterium, and the posttranslational capabilities and low production cost of plants. A similar approach, based on the use of PVX, has also been described recently [16]. Further development and automization of these protocols could increase the throughput of the process and reduce the amount of manpower required.…”

Section: Second-generation Vectors: Deconstructed Vectors and Magnifementioning

confidence: 95%

Viral vectors for the expression of proteins in plants

Gleba

Klimyuk

Marillonnet

2007

Current Opinion in Biotechnology

366

268

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“…This improved version infected systemically and directed high-levels of expression of a variety of recombinant proteins such as GFP and the human islet autoantigen glutamic acid decarboxylase (hGAD65) Avesani et al 2003 ) . Recent studies have shown that a minimal PVX vector in which the triple block and CP were removed can allow for high-level expression (Komorova et al 2006 ;Ravin et al 2008 ) . The target gene was inserted downstream of the fi rst viral subgenomic promoter and transcription of the recombinant PVX was controlled by the 35S promoter.…”

Section: Pvxmentioning

confidence: 97%

Plant Virus-Mediated Expression in Molecular Farming

Wang

2011

Molecular Farming in Plants: Recent Advances and Future Prospects

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Plant viruses have contrasting abilities. On one hand, they can induce gene silencing, termed as virus-induced gene silencing. On the other hand, they have evolved mechanisms that suppress gene silencing and allow the accumulation of very high levels of viral proteins in infected plants. The latter is the driving force for the manipulation of plant viruses for molecular farming in plants. In comparison to the transgenic approach which is often associated with low levels of expression and the requirement of a time-consuming and labour-intensive genetic transformation process, the plant virus-mediated expression approach has several advantages such as easy manipulation, high yield and fast manufacturing. This approach uses plant virus-based expression vectors as a vehicle to produce therapeutic proteins such as antibodies, enzymes, vaccines, and other recombinant proteins of interest in plants. Over the last two decades, a number of plant viruses have been developed and optimized for expression of a variety of pharmaceutical proteins. Some of these recombinant proteins are currently under pre-clinical or clinical trials. In this chapter, I will summarize recent progress, current challenges and future prospects of plant virus-mediated expression in molecular farming.

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New viral vector for efficient production of target proteins in plants

Cited by 39 publications

References 20 publications

Design of virus-based nanomaterials for medicine, biotechnology, and energy

Design of virus-based nanomaterials for medicine, biotechnology, and energy

Viral vectors for the expression of proteins in plants

Plant Virus-Mediated Expression in Molecular Farming

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