New views on three-dimensional imaging technologies for glaucoma: an overview

Abstract

Purpose of review To summarize the literature on three-dimensional (3D) technological advances in ophthalmology, the quantitative methods associated with this, and their improved ability to help detect glaucoma disease progression. Recent findings Improvements in measuring glaucomatous structural changes are the result of dual innovations in optical coherence tomography (OCT) imaging technology and in associated quantitative software. Summary Compared with two-dimensional (2D) OCT parameters, newer 3D parameters provide more data and fewer artifacts.

Ex-vivo-Mikro-CT in der Augenheilkunde: Probenbehandlung und -kontrastierung für die 3D-Darstellung

“…Grundsätzlich stehen mehrere bildgebende Verfahren zur Verfügung, die eine 3-dimensionale Analyse von biologischem Gewebe ermöglichen, sei es am Zell-oder Tiermodell sowie an Humangewebe. In der Augenheilkunde ist die 3D-In-vivo-Bildgebung jedoch auf Verfahren ohne ionisierende Strahlung beschränkt, allen voran die optische Kohärenztomografie (OCT) [9], konfokale Laser-Scanning-Mikroskopie (CLSM) [10], Ultrahochfeld-MRT (UHF-MRT) [11] und hochfrequente Ultraschall-Biomikroskopie (UBM) [12]. Bei Ex-vivo-Gewebe erlaubt Mikro-CT allerdings im Gegensatz zu anderen 3-dimensionalen Analyseverfahren eine zusammenhängende (integrative) Darstellung mehrerer verschiedener Gewebekomponenten über ein größeres Probenvolumen von bis zu mehreren Zentimetern Durchmesser, wie bspw.…”

“…Basically, there are several imaging methods available that allow three-dimensional analysis of biological tissue, whether from cell or animal models, as well as human tissues. In ophthalmology, however, 3D in vivo imaging is limited to procedures without ionizing radiation, primarily optical coherence tomography (OCT) [9], confocal laser scanning microscopy (CLSM) [10], ultra-high-field MRI (UHF MRI) [11], and high-frequency ultrasound biomicroscopy (UBM) [12]. For ex vivo tissue, however, in contrast to other three-dimensional analysis methods, micro-CT allows a coherent (integrative) representation of several different tissue components via a larger sample volume of up to several centimeters in diameter, such as the anterior chamber of the eye, at comparatively high resolution (▶ Fig.…”