[1] The particulate backscattering coefficient b bp is an inherent optical property that plays a central role in studies of ocean color remote sensing. Because of practical difficulties associated with measurements of the volume scattering function (VSF) over the whole backward hemisphere, b bp is currently derived using fixed-angle backscattering sensors and applying a conversion factor for particulate backscattering, referred to as c p . The underlying assumptions of the fixed-angle approach are as follows: (1) in the green band, c p is fairly constant in the angular range 100°-150°and (2) for a fixed scattering angle, c p is wavelength-independent. In this study we investigated the variability of c p based on spectral measurements of the full VSF, both in situ and for algal culture in the laboratory. The in situ data used in our study were acquired in a coastal environment outside of phytoplankton blooms, whereas the laboratory data were representative for phytoplankton bloom conditions in oceanic waters. At 555 nm, c p was found to vary significantly in the angular range 100°-130°, and at 140°, c p was found to be weakly variable in nonblooming waters only. The spectral variability of c p was studied for the first time, and the spectral slopes of c p , measured in situ, were found to vary within ±6%. Under the assumption that c p (140°) is wavelength-independent, the induced error in the estimates of b bp was found to be lower than 10%. The algal culture showed a much higher spectral variability in c p (±20%), which induced an error in the estimates of b bp up to ±25.8%.Citation: Chami, M., E. Marken, J. J. Stamnes, G. Khomenko, and G. Korotaev (2006), Variability of the relationship between the particulate backscattering coefficient and the volume scattering function measured at fixed angles,