New Variants of the B16 Melanoma: Tumorigenicity and Metastatic Properties Under Different Culture Conditions

Aubert, C; Rougé, Françoise; Voulot, C

doi:10.1093/jnci/82.11.952

Cited by 5 publications

(5 citation statements)

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“…After long-term culture (1 year) in EMEM of B16 control, MMBl6, and AMB16 cells, spontaneous pulmonary metastases disappeared in C5Fla, CJC, and Cclc mice (P < 0.005). Thus, we observed that long-term culture was accompanied by the loss of spontaneous metastatic ability, as reported previously (Aubert et al, 1990).…”

Section: Differentiation and Numbers Of Pulmonary Metastases In Albinsupporting

confidence: 88%

“…Our results show that the production of melanin under Merent culture conditions, in the metastatic variant tumors and in pulmonary metagtases, exhibited a marked degree of heterogeneity that was unrelated to the metastatic phenotype. The evolution of the metastatic properties, in the course of sequential passages in cell culture, was manifested as a rapid decrease in the numbers of spontaneous and experimental metastases (Aubert et al, 1990). The B16 melanoma and its metastatic YB16 and MB16 variants, with the progression of tumors in vivo and the loss in vitro of metastatic ability, should prove to be an excellent model system for discrimination between the types of cellular change that occur throughout the stages of varying metastatic potential and differentiation that are related to genetically and epigenetically modulated conditions.…”

Section: Frequency Of Pigmented Metastases (Percentage) In Albino Recmentioning

confidence: 99%

“…'IEunorigenic human and mouse melanocytes exhibit a wide range of biochemical markers, metabolites, and enzymes that are associated with melanogenesis (Agrup et al, 1977;Halaban et al, 1984;Hansson, 1988;Hu, 1985;Hu et al, 1988;Rorsman et al, 1989). A great many lines of human and animal melanomas have been derived from original spontaneous tumors and studied in terms of both their ultrastructural characteristics and the biochemical parameters of melanogenesis (Aubert et al, 1987(Aubert et al, , 1990Berkelhammer et al, 1982;Fidler, 1973;Hearing et al, 1988;Ito et al, 1989;Kreider and Schmoyer, 1975). Furthermore, differentiation and tumorigenicity of human malignant melanocytes have been related to environmental conditions in vivo and in vitro (Aubert et al, 1976(Aubert et al, , 1984.…”

Section: Introductionmentioning

confidence: 99%

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Differentiation of New Metastatic Variants of B16 Melanoma Under Different Culture Conditions

Aubert

Ali-Mehidi

Rougé

et al. 1992

Pigment Cell Research

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The purpose of this study was to examine the differentiation of variant tumors of the B16 metastatic melanoma when tumors were grown serially under different culture conditions and transplanted into C57BL/6J black mice, lethal yellow Ay/a, albino c/c, and C+/c mutant mice. Morphological and biochemical markers of melanogenesis were examined in cells in culture and in the corresponding tumors. Cellular pigmentation was assessed in terms of the levels of DOPA and 5-S-CD and in terms of tyrosinase activity in the various cell lines and tumors. The observed change from high to low metastatic capacity, which was dependent on culture conditions, appeared to be unrelated to melanogenesis even though changes were observed in the biochemical melanotic phenotype. Overall, tumor cells from spontaneous pulmonary metastases appear to differentiate in ways that are unrelated to the instability of experimental metastatic capacity. The melanotic phenotype in albino c/c and C+/c mice was dependent on the phenotype of the parental tumors. A marked difference was observed between two pigmentation compartments, one of which was stable in the B16 control, while the other was unstable in YB16 and MB16 variant cells and in the tumors derived from them. It appears, therefore, that the metastatic capacity of B16 metastatic variants is changeable and is independent of the unstable melanogenic behavior. The production of metastases and the differentiation of tumors in the present experiments appeared to be related to the genetic background of the mice and the epigenetic metabolic environment of tumors and cells.

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Section: Differentiation and Numbers Of Pulmonary Metastases In Albinsupporting

confidence: 88%

Section: Frequency Of Pigmented Metastases (Percentage) In Albino Recmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Differentiation of New Metastatic Variants of B16 Melanoma Under Different Culture Conditions

Aubert

Ali-Mehidi

Rougé

et al. 1992

Pigment Cell Research

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“…Although these conclusions relied upon immunohistologic analysis and antibody ferentiation, adhesive, and metastatic properties of melanoma cells. Studies of tumor growth in syngeneic perturbation experiments, the present study makes use of NG2/HMP-expressing and nonexpressing mouse C57Bl/6 mice show that in the case of both B16F1 and B16F10 cells, expression of NG2 enhances subcutane-proliferative capacity of cells both in vitro and in vivo (Aubert et al, 1990;Tsukamoto et al, 1991). In some ous tumor growth.…”

Section: Discussionmentioning

confidence: 77%

Expression of the NG2 proteoglycan enhances the growth and metastatic properties of melanoma cells

1998

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The human homologue of NG2, the human melanoma proteoglycan (HMP), is expressed on most human melanomas. To investigate the role of this proteoglycan in melanoma progression, we have attempted to identify functionally important molecular ligands for NG2. Immunohistochemical analysis of cell lines that endogenously express NG2/HMP suggests that NG2/HMP associates with CD44 and alpha4beta1 integrin, two molecules previously implicated in melanoma progression. Transfection of rat NG2 into the NG2-negative B16 mouse melanoma cell line also resulted in a highly colocalized pattern of expression between the transfected rat NG2 and the endogenously expressed mouse CD44 and alpha4beta1 integrin molecules. In functional assays, expression of NG2 decreased the adhesion of B16 melanoma cells to CD44 monoclonal antibodies, hyaluronic acid, the C-terminal 40-kDa fibronectin fragment, and the CS1 fibronectin peptide, suggesting that NG2 may negatively modulate CD44- and alpha4beta1-mediated binding events. Expression of NG2 increased the proliferation of melanoma cells in culture and increased tumorigenicity in vivo. Moreover, NG2 expression led to increased lung metastasis of B16F1 and B16F10 melanoma cells in experimental metastasis studies. Together, these studies demonstrate that NG2 is capable of modulating the adhesion, proliferation, and metastatic potential of melanoma cells.

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“…In consonance with this idea, it has been proposed that the concept of dedierentiation in oncology 27 is associated with an increase in the proliferative capacity of the cells both in vitro and in vivo. 1,51 Sometimes this is attributable to changes in the repertoire of cell surface antigens expressed by transformed cells at varying stages of dierentiation. 15 Recent ®ndings show that not only is NG2 expressed in human brain sections 45 , but in our laboratory we have found NG2 +ve cells within human glionia sections.…”

Section: Discussionmentioning

confidence: 99%

The NG2 chondoitin sulfate proteoglycan: role in malignant progression of human brain tumours

Chekenya

Rooprai

Davies

et al. 1999

Intl J of Devlp Neuroscience

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The expression and function of NG2, a transmembrane chondroitin sulfate proteoglycan was studied in human gliomas of various histological types in culture using immunocytochemistry and flow cytometry. NG2 was differentially expressed in the neoplasms, with higher expression in high compared to low-grade gliomas. In acutely isolated cells from human biopsies, NG2 +ve and NG2 -ve populations were morphologically distinct from each other, and NG2 +ve cells were more proliferative than NG2 -ve cells. The mitogens platelet derived growth factor (PDGF-AA) and basic fibroblast growth factor (bFGF) added in combination to serum-free medium (SFM) upregulated NG2 expression on glioblastoma multiforme cells in culture but had little effect on NG2 expression on the anaplastic astrocytoma cells. Furthermore, NG2 was colocalised with the platelet derived growth factor alpha receptor (PDGFalphaR) and antibody blockade of the PDGF-alphaR ablated NG2 expression on the glioblastoma multiforme cells, suggesting that increased NG2 expression in the presence of PDGF-AA is mediated via the PDGF-alphaR. Assays of migration and invasion indicate that NG2 +ve glioma cells migrated more efficiently on collagen IV and that NG2 -ve cells were more invasive than their NG2 +ve counterparts. The results indicate that NG2 may be, respectively, positively and negatively related to the proliferative and invasive capacity of glioma cells. Thus, expression of the NG2 proteoglycan may have major implications for malignant progression in glial neoplasms and may prove a useful target for future therapeutic regimens.

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New Variants of the B16 Melanoma: Tumorigenicity and Metastatic Properties Under Different Culture Conditions

Cited by 5 publications

References 1 publication

Differentiation of New Metastatic Variants of B16 Melanoma Under Different Culture Conditions

Differentiation of New Metastatic Variants of B16 Melanoma Under Different Culture Conditions

Expression of the NG2 proteoglycan enhances the growth and metastatic properties of melanoma cells

The NG2 chondoitin sulfate proteoglycan: role in malignant progression of human brain tumours

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