New validated spectrofluorimetric protocol for colistin assay through condensation with 2,2-dihydroxyindan-1,3-dione: application to content uniformity testing

Attia, Tamer Z.; Abdelmajed, Mahmoud A; Omar, Mahmoud A.; Thagfan, Sultan S. Al; El‐Din, Khalid M. Badr

doi:10.1039/d2ra04259b

Cited by 2 publications

(1 citation statement)

References 27 publications

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“…In the presence of phenylacetaldehyde, ninhydrin is utilized as a derivatizing agent to assay drugs with primary amine groups. producing yellow fluorescent derivatives [29][30][31][32][33][34][35][36]. In the developed experiment, ninhydrin condensed with the amino moiety of Poly B in addition to phenylacetaldehyde at λ em = 475.5 nm (following excitation at λ ex .…”

Section: Resultsmentioning

confidence: 99%

Condensation methodology for quantification of Polymyxin B fluorimetrically: application to pharmaceutical formulations and greenness assessment

Abdelmajed,

El-Din,

Attia

et al. 2024

BMC Chemistry

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The appearance of multidrug-resistant Gram-negative bacterial infections, along with the lack of newly discovered antibiotics, resulted in the return to old antimicrobial medications like Polymyxins. As a result, the suggested technique aims to develop a fast, environmentally friendly, and sensitive fluorimetric method for quantifying Polymyxin B. The investigated approach depends on generating a highly fluorescent derivative by a condensation pathway between the studied drug and ninhydrin in the presence of phenylacetaldehyde and then estimated spectrofluorimetrically. After the reaction conditions were well optimized, the fluorescent product was estimated at emission wavelength (λem) = 475.5 nm (following excitation at a wavelength (λex) = 386 nm. The developed calibration plot displayed rectilinear throughout the following range (0.2-3 µg mL− 1), and the calculated limit of detection and quantification were 0.062 µg mL− 1 and 0.187 µg mL− 1, respectively. As a consequence, the drug’s ophthalmic and intravenous pharmaceutical forms were both successfully quantified with an excellent degree of recovery. Finally, the methodology’s greenness was assessed utilizing Analytical Eco-Scale scores.

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Section: Resultsmentioning

confidence: 99%

Condensation methodology for quantification of Polymyxin B fluorimetrically: application to pharmaceutical formulations and greenness assessment

Abdelmajed,

El-Din,

Attia

et al. 2024

BMC Chemistry

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Full green assay of parenteral dosage forms of polymyxins utilizing xanthene dye: application to content uniformity testing

Abdelmajed,

El-Din,

Attia

et al. 2024

BMC Chemistry

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Due to the lack of other treatment options, a rebirth of polymyxins is urgently required. Colistin (also called polymyxin E) and polymyxin B are the only two examples of this antibiotic class that were effectively employed in such critical situations. In the present work, both of the two studied medications were quantified via a simple, green, and non-extracting spectrophotometric approach based on the formation of ion-pair complexes with Erythrosine B. Without using any organic solvents, the pink color of the created complexes was detected at wavelength = 558 nm. To achieve the highest intensity of absorbance, optimum conditions were established by the screening of many experimental factors such as pH, buffer volume, the volume of Erythrosine B, and the time consumed to undergo the reaction. For Colistin and Polymyxin B respectively, Beer-Lambert’s law was observed at the concentration ranges of 1–6, 1–9 µg mL− 1. The technique was approved and validated following ICH recommendations. Lastly, the suggested approach has been successfully implemented to quantify the cited medications colorimetrically, for the first time, in their parenteral dosage forms with excellent recoveries. Also, Content uniformity testing was implemented.

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New validated spectrofluorimetric protocol for colistin assay through condensation with 2,2-dihydroxyindan-1,3-dione: application to content uniformity testing

Abstract: Spectrofluorimetric assay of colistin in authentic and pharmaceutical market forms through condensation with ninhydrin. Application of content uniformity testing.

Cited by 2 publications

References 27 publications

Condensation methodology for quantification of Polymyxin B fluorimetrically: application to pharmaceutical formulations and greenness assessment

Condensation methodology for quantification of Polymyxin B fluorimetrically: application to pharmaceutical formulations and greenness assessment

Full green assay of parenteral dosage forms of polymyxins utilizing xanthene dye: application to content uniformity testing

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