New Uses of Vinyl Tape for Reliable Collection and Diagnosis of Common Superficial Mycoses

Miranda, Milton Edson; Silva, Ademir J.G.

doi:10.1111/j.1540-9740.2003.02057.x

Cited by 11 publications

(11 citation statements)

References 8 publications

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“…In 1953, Porto first used cellophane tape for the diagnosis of tinea versicolor, 6 and since then, there have been a few other studies supporting its use. [7][8][9][10] In 1974, Milne et al reported the superiority of cellophane method for demonstration of dermatophytes ( 4 In the current study, long, undulating, refractile and glistening fungal elements were visualized in both methods either as an intricate network of hyphal filaments or as sparsely distributed isolated strands (Fig. 1b,c).…”

Section: Discussionmentioning

confidence: 59%

“…In 1953, Porto first used cellophane tape for the diagnosis of tinea versicolor, and since then, there have been a few other studies supporting its use . In 1974, Milne et al .…”

Section: Discussionmentioning

confidence: 99%

“…It is a simpler technique that requires less time to do, and can be performed even in the absence of clinically perceptible scales. Importantly, it alleviates the need for a scalpel blade, which is often a cause of undue concern, especially in paediatric patients . Avoidance of sharp instruments also reduces the risk of inadvertent trauma and of spread of infection to the surrounding uninvolved skin.…”

Section: Discussionmentioning

confidence: 99%

“…Artefacts such as KOH crystals in the conventional method pose an additional disadvantage, precluding easy diagnosis, especially by untrained individuals. Besides being an easier technique, the cellophane tape method permits storage of slides for a longer period and allays the need for complex storage conditions . However the limitation of this technique is that it is unsuitable for sample collection in very moist areas and is also unsuitable for onychomycoses .…”

Section: Discussionmentioning

confidence: 99%

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Potassium hydroxide mount with cellophane adhesive tape: a method for direct diagnosis of dermatophyte skin infections

Raghukumar

Ravikumar

2018

Clin Exp Dermatol

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Section: Discussionmentioning

confidence: 59%

“…In 1953, Porto first used cellophane tape for the diagnosis of tinea versicolor, and since then, there have been a few other studies supporting its use . In 1974, Milne et al .…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Potassium hydroxide mount with cellophane adhesive tape: a method for direct diagnosis of dermatophyte skin infections

Raghukumar

Ravikumar

2018

Clin Exp Dermatol

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“…11,12 These methods may be traumatic to young children, and 2 other methods have been evaluated and proven efficacious in children-the toothbrush method and the cotton swab method. Samples can be obtained by several methods including plucking hair, scraping skin and hair with a scalpel blade, brushing hair, or using adhesive tape.…”

Section: Diagnosismentioning

confidence: 99%

The Assessment and Management of Tinea Capitis in Children

&NA;¹

2007

Pediatric Emergency Care

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Tinea capitis is an infection of the scalp and hair shaft caused by dermatophyte fungi. It is seen in all age groups, and the incidence seems to be on the rise in North America. Clinical diagnosis of tinea capitis can be challenging, as symptoms can vary from minimal pruritus with no hair loss, to severe tenderness, purulence, and permanent scarring in inflammatory kerion lesions. The diagnosis of tinea capitis must be confirmed in the laboratory by using fungal stains or obtaining cultures, since treatment may be prolonged with potential side effects. Systemic therapy is needed because topical antifungals cannot effectively penetrate the hair shaft to eradicate the infection. Oral griseofulvin remains the standard treatment agent, but terbinafine and itraconazole are also effective alternatives (although currently without Food and Drug Administration approval).

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Conventional Methods for the Diagnosis of Dermatophytosis

2008

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Dermatophytes are keratinolytic fungi responsible for a large variety of diseases that can affect glabrous skin, nails and hair. In many cases, the diagnosis is not clinically obvious, and mycological analysis is required. This includes both direct microscopic examination and cultures. First of all, clinical specimens have to be sampled according to localization and characteristics of the lesions. Direct microscopic examination is usually performed using clearing reagents (KOH or Amman's chloral-lactophenol), but its sensitivity may be greatly enhanced by the use of stains or fluorochromes such as Congo red or Calcofluor white. Histological analysis is an efficient method, but it is constraining for the patients and, as direct examination, it does not allow precise identification of the pathogen. Cultures are therefore needed, and specific culture media may be used to overcome the growth of rapidly growing contaminating moulds which may hamper the recovery of dermatophytes. Identification at the species level which may be useful to initiate an appropriate treatment or for setting prophylactic measures, relies on macroscopic and microscopic morphology. Subcultures on culture media which stimulate conidiation and, for some species, the production of pigments, are often necessary. Additionally, in case of atypical isolates, some biochemical or physiological tests may be performed such as the search for urease activity or the in vitro hair perforation test. However, their contribution to species identification is rather limited, and progress is still needed for the development of biochemical or immunological tests allowing an accurate identification at the species level, pending for the availability of molecular biology-based kits.

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New Uses of Vinyl Tape for Reliable Collection and Diagnosis of Common Superficial Mycoses

Abstract: In addition to improved patient compliance, ease of performance, and low cost, the technique seems appropriate for using in an office environment during a routine medical visit. Whenever vinyl tape specimens yield negative results, a scraping-sampled preparation should be provided.

Cited by 11 publications

References 8 publications

Potassium hydroxide mount with cellophane adhesive tape: a method for direct diagnosis of dermatophyte skin infections

Potassium hydroxide mount with cellophane adhesive tape: a method for direct diagnosis of dermatophyte skin infections

The Assessment and Management of Tinea Capitis in Children

Conventional Methods for the Diagnosis of Dermatophytosis

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