New Trends in Immunoassays

Chan, Cangel Pui-yee; Cheung, Yiu-chi; Renneberg, Reinhard; Seydack, Matthias

doi:10.1007/10_2007_075

Cited by 53 publications

(40 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The lower limit of PSA in this method was 100 fg/mL, which is 1000 times more sensitive than ELISA. Compared with immunodetection (1 ng/mL), the method does not require specialized equipment and safeguards (12). Also, the method is simple and quick.…”

Section: Nano-nucleic Acid Barcode Dot Detectionmentioning

confidence: 99%

A novel sensitive immunoassay by nucleic acid barcode dot and its application in the detection of prostate-specific antigen

Kong

Zhou

et al. 2009

Clinical Chemistry and Laboratory Medicine

View full text Add to dashboard Cite

Background: The sensitivity and selectivity of traditional methods limits ultramicro detection of proteins. Bio-barcode amplification detection methods based on nanotechnology enables ultramicro detection of protein. However, bio-barcode amplification detection depends on the oligonucleotides being fixed on a glass chip. It also requires specialized equipment, which limits its application. We introduce a nanonucleic acid barcode dot detection technology to determine ultramicro concentrations of protein. The method is simple, quick and accurate. Methods: Magnetic probe (IgG-M) and dual-labeled gold nanoparticle bio-probe (IgG-Au-DNA) were prepared. Protein was captured using a sandwich assay technique and magnetic separation was used. The DNA barcode was released with dithiothreitol (DTT) and detected directly without the requirement for polymerase chain reaction (PCR). Serum prostate-specific antigen (PSA) from 135 patients was detected with this method and compared with enzyme linked immunosorbent assay (ELISA) and radioimmunoassay (RIA). Results: Each IgG-Au-DNA could be covered with 138"47 oligonucleotides and 11"3 antibodies. The IgG-M could

show abstract

Section: Nano-nucleic Acid Barcode Dot Detectionmentioning

confidence: 99%

A novel sensitive immunoassay by nucleic acid barcode dot and its application in the detection of prostate-specific antigen

Kong

Zhou

et al. 2009

Clinical Chemistry and Laboratory Medicine

View full text Add to dashboard Cite

show abstract

“…Magnetic beads are nano-sized or micro-sized ferromagnetic iron oxide particles. Functionalized magnetic beads coated with antibodies or oligonucleotides have been widely used for the purification, extraction and detection of biomolecules (26,27). We herein report a general method for RNA extraction based on magnetic beads and capturer strands, and its application for prostate cancer screening and diagnosis.…”

Section: Introductionmentioning

confidence: 99%

Improved sensitivity and specificity for prostate cancer diagnosis based on the urine PCA3/PSA ratio acquired by sequence-specific RNA capture

Zheng

Dou

et al. 2015

Oncology Reports

View full text Add to dashboard Cite

Abstract. Prostate cancer antigen 3 (PCA3) is a non-coding RNA fragment that is overexpressed in prostate cancer cells. However, the clinical applications of PCA3 are highly limited due to the instability of RNA and the lack of reliable and efficient RNA extraction and purification methods. Thus, in the present study, we compared three different methods of RNA extraction to further confirm the higher yield of commercial magnetic beads with poly-T functionalization and a capturer strand. The current protocols for RNA extraction of i) the phenol-chloroform method, ii) the affinity column method and iii) magnetic beads with poly-T functionalization and a capturer strand were applied separately for RNA extraction in urine samples. Reverse transcription-quantitative polymerase chain reaction was performed to evaluate the yield of the three methods of RNA extraction. Furthermore, 52 urine samples after prostate massage from patients suspected of a diagnosis of prostate cancer were collected. The Mag-Cap method and RT-PCR were applied to obtain the PCA3 score. The clinical value of the PCA3 score was investigated by comparison with the pathology of the prostate biopsy. The yield of the Mag-Cap method was higher than that of the phenol-chloroform method and commercial kits. Thirty-four patients were pathologically diagnosed with prostate cancer and 18 with benign prostatic hyperplasia (BPH). It was confirmed that the median PCA3 score was higher among the prostate cancer patients than those with benign disease (53.5 vs. 17, p=0.000). A sensitivity of 82.4% and a specificity of 77.8% were obtained when the cut-off value for the PCA3 score was 28.5. The Mag-Cap method was found to be more efficient for RNA extraction. The urinary PCA3 score is a promising method for prostate cancer screening, detection and diagnosis, and has the potential to reduce unnecessary prostate biopsies.

show abstract

“…Today, immunochemical methods represent a core diagnostic technique that will be available for increasing numbers of applications [2][3][4][5][6]. Research in immunodiagnostics relies heavily upon analytical chemistry and nanotechnology [7].…”

mentioning

confidence: 99%

Immunodiagnostics and Immunosensor Design

Gubala¹,

Klein²,

Templeton³

et al. 2016

IUPAC Standards Online

View full text Add to dashboard Cite

This work compiles information on the principles of diagnostic immunochemical methods and the recent advances in this field. It presents an overview of modern techniques for the production of diagnostic antibodies, their modification with the aim of improving their diagnostic potency, the different types of immunochemical detection systems, and the increasing diagnostic applications for human health that include specific disease markers, individualized diagnosis of cancer subtypes, therapeutic and addictive drugs, food residues, and environmental contaminants. A special focus lies in novel developments of immunosensor techniques, promising approaches to miniaturized detection units and the associated microfluidic systems. The trends towards high-throughput systems, multiplexed analysis, and miniaturization of the diagnostic tools are discussed. It is also made evident that progress in the last few years has largely relied on novel chemical approaches.

show abstract

New Trends in Immunoassays

Cited by 53 publications

References 54 publications

A novel sensitive immunoassay by nucleic acid barcode dot and its application in the detection of prostate-specific antigen

A novel sensitive immunoassay by nucleic acid barcode dot and its application in the detection of prostate-specific antigen

Improved sensitivity and specificity for prostate cancer diagnosis based on the urine PCA3/PSA ratio acquired by sequence-specific RNA capture

Immunodiagnostics and Immunosensor Design

Contact Info

Product

Resources

About