New trends in gamete's cryopreservation

Arav, Amir; Yavin, S.; Zeron, Yoel; Natan, Dity; Dekel, Izik; Gacitua, H.

doi:10.1016/s0303-7207(01)00700-6

Cited by 178 publications

(125 citation statements)

References 16 publications

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“…These findings of the beneficial impact of DF on sperm quality are in Benefits derived from DF are believed to be due in part to the reduction of mechanical trauma to the sperm membrane during ice crystal formation. This is accomplished through a controlled seeding or nucleation process that results in laminar as opposed to random (straw method) ice crystal formation (Arav et al 2002). In addition, the linear controlled nucleation process eliminates the freezing point plateau typically observed with straw freezing systems, thus reducing the exposure time of sperm cells to potentially harmful supersaturated cryopreservation media (Mazur 1984, O'Brien & Robeck 2006.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of motility, membrane status and DNA integrity of frozen–thawed bottlenose dolphin (Tursiops truncatus) spermatozoa after sex-sorting and recryopreservation

Montano¹,

Kraemer²,

Love³

et al. 2012

Reproduction

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Artificial insemination (AI) with sex-sorted frozen-thawed spermatozoa has led to enhanced management of ex situ bottlenose dolphin populations. Extended distance of animals from the sorting facility can be overcome by the use of frozen-thawed, sorted and recryopreserved spermatozoa. Although one bottlenose dolphin calf had been born using sexed frozen-thawed spermatozoa derived from frozen semen, a critical evaluation of in vitro sperm quality is needed to justify the routine use of such samples in AI programs. Sperm motility parameters and plasma membrane integrity were influenced by stage of the sex-sorting process, sperm type (non-sorted and sorted) and freezing method (straw and directional) (P!0.05). After recryopreservation, sorted spermatozoa frozen with the directional freezing method maintained higher (P!0.05) motility parameters over a 24-h incubation period compared to spermatozoa frozen using straws. Quality of sperm DNA of non-sorted spermatozoa, as assessed by the sperm chromatin structure assay (SCSA), was high and remained unchanged throughout freeze-thawing and incubation processes. Though a possible interaction between Hoechst 33342 and the SCSA-derived acridine orange was observed in stained and sorted samples, the proportion of sex-sorted, recryopreserved spermatozoa exhibiting denatured DNA was low (6.6G4.1%) at 6 h after the second thawing step and remained unchanged (PO0.05) at 24 h. The viability of sorted spermatozoa was higher (P!0.05) than that of non-sorted spermatozoa across all time points after recryopreservation. Collective results indicate that bottlenose dolphin spermatozoa undergoing cryopreservation, sorting and recryopreservation are of adequate quality for use in AI.

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Section: Discussionmentioning

confidence: 99%

Evaluation of motility, membrane status and DNA integrity of frozen–thawed bottlenose dolphin (Tursiops truncatus) spermatozoa after sex-sorting and recryopreservation

Montano¹,

Kraemer²,

Love³

et al. 2012

Reproduction

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“…One method, termed multi-thermal gradient (MTG), has been reported (Arav et al, 2002). This freezing technology is based on directional freezing.…”

Section: Advances In Freezing Semenmentioning

confidence: 99%

Integration of future biotechnologies into the equine industry

Squires¹

2005

Animal Reproduction Science

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There has and will continue to be reproductive techniques available that have a positive impact upon the equine breeding industry. This review focuses on semen technologies that have been developed or are in the process of being developed. The use of fluorescent dyes and flow cytometry has provided the researcher and clinician with powerful tools to evaluate several sperm attributes. These procedures have been utilized to evaluate sperm viability, acrosome status, mitochondrial status, DNA integrity and stages of capacitation. Flow cytometry allows several sperm attributes to be evaluated on thousands of spermatozoa in a matter of seconds. Development of procedures for insemination of mares with relatively small numbers of spermatozoa has the potential to change how stallions and their semen are managed. This review discusses the use of insemination of fresh, frozen and sex-sorted spermatozoa in relatively small numbers compared with conventional insemination technologies. The recent acceptance of frozen-thawed semen by many of the major breed registries has stimulated an increase in research on frozen semen. Many of the studies have focused on identifying damage during the freezing and thawing process. Numerous studies also have been conducted to modify freezing extenders so that the sperm are protected during the freezing and thawing process. The production of in vitro-produced embryos is extremely limited in the horse due to the failure of in vitro fertilization. However, intracytoplasmic sperm injection (ICSI) has been used for the production of foals from stallions that have less than typical sperm numbers or from stallions that have died and a limited quantity of frozen semen is available. This technique has been used by several laboratories to produce embryos in vitro. The breeder and veterinarian now have access to techniques that allow assessment of semen quality, improvement of procedures for freezing and thawing and insemination of mares with fewer numbers of spermatozoa. It is likely that the next decade will also produce tremendous advances in semen technologies that can be utilized in the horse industry.

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“…The development of cryopreservation technology in nonhuman mammalian oocytes can be applied to humanassisted reproductive technology, in which oocyte cryopreservation shows many advantages for infertility treatment (Arav et al 2002). Recently, vitrification has been widely used to cryopreserve oocytes in many mammals (Kono et al 1991, Arav & Zeron 1997, Isachenko et al 1998, including man (Kuleshova et al 1999, Yoon et al 2000.…”

Section: Introductionmentioning

confidence: 99%

Improved development by Taxol pretreatment after vitrification of in vitro matured porcine oocytes

Shi¹,

Zhu

Zhang³

et al. 2006

Reproduction

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This study was designed to examine the effect of Taxol pretreatment on vitrification of porcine oocytes matured in vitro by an open pulled straw (OPS) method. In the first experiment, the effect of Taxol pretreatment and fluorescein diacetate (FDA) staining on parthenogenetic development of oocytes was evaluated. In the second experiment, viability, microtubule organization and embryo development of oocytes were assessed after oocytes were exposed to vitrification/warming solutions or after vitrification with or without Taxol pretreatment. The results showed that Taxol pretreatment and/or FDA staining did not negatively influence the oocyte's developmental competence after parthenogenetic activation. After being exposed to vitrification/warming solutions, the survival rate (83.3%) of the oocytes was significantly (P < 0.05) reduced as compared with that in the control (100%). Vitrification/warming procedures further reduced the survival rates of oocytes regardless of oocytes being treated with (62.1%) or without (53.8%) Taxol. The proportions of oocytes with normal spindle configuration were significantly reduced after the oocytes were exposed to vitrification/warming solutions (38.5%) or after vitrification with (10.3%) or without (4.1%) Taxol pretreatment as compared with that in control (76.8%). The rates of two-cell-stage (5.6-53.2%) embryos at 48 h and blastocysts (0 -3.8%) at 144 h after activation were significantly reduced after exposure to vitrification/warming solutions or after vitrification as compared with control (90.9% and 26.6% respectively). However, the proportion of vitrified oocytes developed to two-cell stage was significantly higher when oocytes were pretreated with (24.3%) than without (5.6%) Taxol. These results indicate that pretreatment of oocytes with Taxol before vitrification helps to reduce the damage induced by vitrification and is a potential way to improve the development of vitrified porcine oocytes. Reproduction (2006) 131 795-804

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New trends in gamete's cryopreservation

Cited by 178 publications

References 16 publications

Evaluation of motility, membrane status and DNA integrity of frozen–thawed bottlenose dolphin (Tursiops truncatus) spermatozoa after sex-sorting and recryopreservation

Evaluation of motility, membrane status and DNA integrity of frozen–thawed bottlenose dolphin (Tursiops truncatus) spermatozoa after sex-sorting and recryopreservation

Integration of future biotechnologies into the equine industry

Improved development by Taxol pretreatment after vitrification of in vitro matured porcine oocytes

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