New Treatment of Wound Healing With Allogenic Acellular Human Skin Graft: Preclinical Assessment and In Vitro Study

Nita, Magdalena; Pliszczyński, Jacek; Kowaléwski, Cezary; Woźniak, Katarzyna; Eljaszewicz, Andrzej; Moniuszko, Marcin; Kamiński, Artur; Śladowski, Dariusz; Zimek, Z.; Majewski, Sławomir; Kosieradzki, Maciej; Fiedor, Piotr

doi:10.1016/j.transproceed.2020.02.115

Cited by 9 publications

(5 citation statements)

References 33 publications

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“…Having established the high customizability and effectiveness of keystone elements of ADMs, on their own they hold a great deal of potential. Even so, skin substitutes' therapeutic potential may be improved by the supplemental utilization of stem and progenitor cells with well-characterized activities supporting the healing process, as in the case of mesenchymal stem cells (MSCs) and fibroblasts [103,108,109]. Interestingly MSCs co-cultured with skin substitutes in vitro were shown to release trophic factors important for tissue regeneration [110] and to improve the healing of diabetic wounds when used in tandem skin substitutes, including ADMs [76,79,[110][111][112].…”

Section: Cell-supplemented Dermal Matricesmentioning

confidence: 99%

Chronic Diabetic Wounds and Their Treatment with Skin Substitutes

Holl

Kowaléwski

Zimek

et al. 2021

Cells

137

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With the global prevalence of type 2 diabetes mellitus steeply rising, instances of chronic, hard-healing, or non-healing diabetic wounds and ulcers are predicted to increase. The growing understanding of healing and regenerative mechanisms has elucidated critical regulators of this process, including key cellular and humoral components. Despite this, the management and successful treatment of diabetic wounds represents a significant therapeutic challenge. To this end, the development of novel therapies and biological dressings has gained increased interest. Here we review key differences between normal and chronic non-healing diabetic wounds, and elaborate on recent advances in wound healing treatments with a particular focus on biological dressings and their effect on key wound healing pathways.

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Section: Cell-supplemented Dermal Matricesmentioning

confidence: 99%

Chronic Diabetic Wounds and Their Treatment with Skin Substitutes

Holl

Kowaléwski

Zimek

et al. 2021

Cells

137

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“…Among them, a treatment protocol based on the grafting of autologous skin after adequate debridement through negative pressure wound therapy or hyperbaric oxygen therapy gave promising results in immunosuppressed patients [ 2 , 3 ]; we previously reported on positive outcome with glycerolized skin on a PG lesion [ 4 ]. Recently, grafting of an acellular dermal matrix for a deep PG wound was described [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

“…We here report our preliminary experience of two cases successfully treated with a composite grafting technique based on acellular dermal matrix and glycerolized skin prepared in the Skin Bank Unit of Siena Hospital [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

Successful Treatment of Pyoderma Gangrenosum With Composite Grafting of Acellular Dermal Matrix and Glycerolized Skin: A Preliminary Experience

Tognetti,

Calabrese,

Pianigiani

et al. 2024

Dermatol Pract Concept

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“…However, despite the advances in tissue bioengineering, skin allografts continue to serve as the “gold standard” for the temporary coverage of extensive and deep burns [ 12 ]. The allografts have several desirable properties since they present the capacity to adhere the wound course, providing a better closure, promoting the retention of humidity while improving the thermoregulation of the grafted location [ 13 – 16 ].…”

Section: Introductionmentioning

confidence: 99%

Sterilized human skin graft with a dose of 25 kGy provides a privileged immune and collagen microenvironment in the adhesion of Nude mice wounds

et al. 2022

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This study aimed to report the effects of different doses of ionizing radiation on inflammatory and repair stage of human skin graft adherence in Nude mice wounds. Animals were divided into transplanted with irradiated human skin grafts (IHSG) at 25 and 50 kGy (IHSG 25 kGy; IHSG 50 kGy) and non-IHSG and euthanized on the 3rd, 7th and 21st days after the surgery, by gross and microscopic changes, immunostaining for human type I collagen (Col I) and mouse Col I and Col III and inflammatory cells. We found an effectiveness of human split-thickness graft adherence in mice transplanted with IHSG 25 kGy, as well decrease in dermo-epidermal necrosis and neutrophils, lower loss of skin thickness, epithelization and neo-vascularization. Day 21 post-transplantation with IHSG 25 kGy was observed a well-preserved human skin in the border of the graft, a prominent granulation tissue in an organization by proliferated fibroblasts, Col III deposition and increased B-cells and macrophages. A complete adherence of human skin graft occurred with IHSG 25 kGy. We suggest that the ionizing radiation at 25 kGy mediates inflammation and the repair stage of human skin graft adherence in murine model, thus emerging as a potential tool in healing cutaneous wounds.

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New Treatment of Wound Healing With Allogenic Acellular Human Skin Graft: Preclinical Assessment and In Vitro Study

Cited by 9 publications

References 33 publications

Chronic Diabetic Wounds and Their Treatment with Skin Substitutes

Chronic Diabetic Wounds and Their Treatment with Skin Substitutes

Successful Treatment of Pyoderma Gangrenosum With Composite Grafting of Acellular Dermal Matrix and Glycerolized Skin: A Preliminary Experience

Sterilized human skin graft with a dose of 25 kGy provides a privileged immune and collagen microenvironment in the adhesion of Nude mice wounds

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