New tools to mitigate drug resistance in Enterobacteriaceae – Escherichia coli and Klebsiella pneumoniae

Sundaramoorthy, Niranjana Sri; Shankaran, Prakash; Gopalan, Vidhya; Nagarajan, Saisubramanian

doi:10.1080/1040841x.2022.2080525

Cited by 8 publications

(2 citation statements)

References 174 publications

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“…This gene has been shown to control the appearance of numerous genes that are responsible in antibiotic resistance, including the acrAB efflux pump and the ompK36 porin. 18 It has the capability of binding to the promoters' region in the acrAB efflux pump gene and repress it is expression. It is also binds to the promoters' region in the ompK36 porin which activates its expression.…”

Section: Detection Of Mrka Genementioning

confidence: 99%

Prevalence and molecular detection of Klebsiella pneumoniae isolated from children with infectious diarrhoea in AL-Ramadi city

Al-Omar

2024

IJMMTD

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: Infectious diarrhoea result from a wide range of bacteria, viruses and parasites. This condition is also identified as gastroenteritis, is a well-known as one of most common bacterial pathogens causing gastroenteritis. This study aims to estimate the incidence and identifying both the phenotypic and genotypic characterization of causing gastroenteritis in children under the age of five in al-Ramadi Maternity and Children Teaching Hospital.: Stool samples were collected for 106 children suffering from gastroenteritis, Cultural and microscopical approaches were used for selection, its characteristic features were confirmed using the Vitek2 compact system, anti-microbial sensitivity test, and biofilm production test. furthermore, DNA extracted, purification and Polymerase chain reaction (PCR) were accomplished for genotypic confirmation. In the presented research, stool samples were collected for 106 children suffering from gastroenteritis, and 100 samples were identified as source for bacterial gastroenteritis. The bacterium under consideration () has a percentage of 30%. This isolate revealed resistance to Ceftazidime (80%), nalidixic acid (33%), amikacin (36%), Azithromycin (20%), vancomycin (10%), and Imipenem (6%). Likewise, four genes in isolate were studied via PCR and the results indicates htrA, iss, Mrka and rmpA were 15 (50%), 9 (30%), 6 (20%), 0 (0%) respectively. The results of biofilm production for exposed that 3 (10%) were strong, 10 (33%) moderate, 7 (24%) weak, and 10 (33%) non-producers. : The presented research displayed the bacterium under consideration () has a higher resistance rate to the commonly antibiotics used for bacterial gastroenteritis. In addition, (under consideration) with high resistance to antibiotics showed resistance genes in PCR, in addition to strong biofilm production.

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Section: Detection Of Mrka Genementioning

confidence: 99%

Prevalence and molecular detection of Klebsiella pneumoniae isolated from children with infectious diarrhoea in AL-Ramadi city

Al-Omar

2024

IJMMTD

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“…A major difference in the sensitivity between biofilm-embedded cells and cells in the planktonic state belonging to the same strain has been reported [ 20 , 24 ]. Together, these observations stress the importance of considering biofilm formation ability as a vital bacterial determinant in the planning of UTI treatment [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

Correlation between antimicrobial resistance, biofilm formation, and virulence determinants in uropathogenic Escherichia coli from Egyptian hospital

Alshaikh,

El-banna,

Sonbol

et al. 2024

Ann Clin Microbiol Antimicrob

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Background Uropathogenic Escherichia coli (UPEC) is the main etiological agent behind community-acquired and hospital-acquired urinary tract infections (UTIs), which are among the most prevalent human infections. The management of UPEC infections is becoming increasingly difficult owing to multi-drug resistance, biofilm formation, and the possession of an extensive virulence arsenal. This study aims to characterize UPEC isolates in Tanta, Egypt, with regard to their antimicrobial resistance, phylogenetic profile, biofilm formation, and virulence, as well as the potential associations among these factors. Methods One hundred UPEC isolates were obtained from UTI patients in Tanta, Egypt. Antimicrobial susceptibility was assessed using the Kirby-Bauer method. Extended-spectrum β-lactamases (ESBLs) production was screened using the double disk synergy test and confirmed with PCR. Biofilm formation was evaluated using the microtiter-plate assay and microscopy-based techniques. The phylogenetic groups of the isolates were determined. The hemolytic activity, motility, siderophore production, and serum resistance of the isolates were also evaluated. The clonal relatedness of the isolates was assessed using ERIC-PCR. Results Isolates displayed elevated resistance to cephalosporins (90–43%), sulfamethoxazole-trimethoprim (63%), and ciprofloxacin (53%). Ninety percent of the isolates were multidrug-resistant (MDR)/ extensively drug-resistant (XDR) and 67% produced ESBLs. Notably, there was an inverse correlation between biofilm formation and antimicrobial resistance, and 31%, 29%, 32%, and 8% of the isolates were strong, moderate, weak, and non-biofilm producers, respectively. Beta-hemolysis, motility, siderophore production, and serum resistance were detected in 64%, 84%, 65%, and 11% of the isolates, respectively. Siderophore production was correlated to resistance to multiple antibiotics, while hemolysis was more prevalent in susceptible isolates and associated with stronger biofilms. Phylogroups B2 and D predominated, with lower resistance and stronger biofilms in group B2. ERIC-PCR revealed considerable diversity among the isolates. Conclusion This research highlights the dissemination of resistance in UPEC in Tanta, Egypt. The evident correlation between biofilm and resistance suggests a resistance cost on bacterial cells; and that isolates with lower resistance may rely on biofilms to enhance their survival. This emphasizes the importance of considering biofilm formation ability during the treatment of UPEC infections to avoid therapeutic failure and/or infection recurrence.

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Antimicrobial activity in Asterceae: The selected genera characterization and against multidrug resistance bacteria

Gou¹,

Lu²,

Xie³

et al. 2023

Heliyon

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New tools to mitigate drug resistance in Enterobacteriaceae – Escherichia coli and Klebsiella pneumoniae

Cited by 8 publications

References 174 publications

Prevalence and molecular detection of Klebsiella pneumoniae isolated from children with infectious diarrhoea in AL-Ramadi city

Prevalence and molecular detection of Klebsiella pneumoniae isolated from children with infectious diarrhoea in AL-Ramadi city

Correlation between antimicrobial resistance, biofilm formation, and virulence determinants in uropathogenic Escherichia coli from Egyptian hospital

Antimicrobial activity in Asterceae: The selected genera characterization and against multidrug resistance bacteria

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