New Technologies for Insect-Resistant and Herbicide-Tolerant Plants

Lombardo, Luca; Coppola, Gerardo; Zelasco, Samanta

doi:10.1016/j.tibtech.2015.10.006

Cited by 65 publications

(56 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…45 Notably, ODM has been used to develop a non-transgenic plant breeding technology that has led to a commercial, non-transgenic herbicide-resistant canola. 46 …”

Section: Oligonucleotide-directed Mutagenesismentioning

confidence: 99%

Editing the Genome Without Double-Stranded DNA Breaks

2017

View full text Add to dashboard Cite

Genome editing methods have commonly relied on the initial introduction of double-stranded DNA breaks (DSBs), resulting in stochastic insertions, deletions, and translocations at the target genomic locus. To achieve gene correction, these methods typically require the introduction of exogenous DNA repair templates and low-efficiency homologous recombination processes. In this perspective we describe alternative, mechanistically motivated strategies to perform chemistry on the genome of unmodified cells without introducing DSBs. One such strategy, base editing, uses chemical and biological insights to directly and permanently convert one target base pair to another. Despite its recent introduction, base editing has already enabled a number of new capabilities and applications in the genome editing community. We summarize these advances here and discuss the new possibilities that this method has unveiled, concluding with a brief analysis of future prospects for genome and transcriptome editing without double-stranded DNA cleavage.

show abstract

“…45 Notably, ODM has been used to develop a non-transgenic plant breeding technology that has led to a commercial, non-transgenic herbicide-resistant canola. 46 …”

Section: Oligonucleotide-directed Mutagenesismentioning

confidence: 99%

Editing the Genome Without Double-Stranded DNA Breaks

2017

View full text Add to dashboard Cite

show abstract

“…"In the short term, the development of new techniques or the improvement of existing ones will provide further instruments to counter the appearance of resistant weeds and insects and to reduce the use of agrochemicals" [43].…”

Section: Biotechnology Engineering and Applications In Pharmacologymentioning

confidence: 99%

Review. Development, Applications, Benefits, Challenges and Limitations of the New Genome Engineering Technique. An Update Study

Crauciuc

Tripon

Gheorghiu

et al. 2017

Acta Medica Marisiensis

View full text Add to dashboard Cite

We assume that the CRISPR Cas9 theory must be delimited by applicability, because the consequences of long term DNA manipulation remain unknown. Moreover, the irreversibility of this procedure should instigate researchers to reserved opinions. Usefulness as well as benefits of CRISPR Cas9 made it one of the most popular and used genome editing technique. But with its huge potential, ethical and safety concerns emerge. Therefore, before continuing research in this direction we should have a well organized system that is able to make that differentiation between research and reproduction. However we truly believe in the future of genetic engineering and with the CRISPR-Cas9 system we expect that the opportunity of treating now so called incurable diseases arises. Time is all we need.

show abstract

“…For example, in response to the increasing prevalence of glyphosate-resistant weed biotypes, there has been increased interest in providing weed management solutions through the development of new transgenic crops stacked with herbicide resistant traits to auxinic herbicides, as well as to herbicides that inhibit acetolactate synthase (ALS), acetyl-CoA carboxylase (ACCase) and hydroxyphenylpyruvate dioxygenase (HPPD), stacked with glyphosate and/or glufosinate resistance [68]. This idea has been promoted as a sustainable approach to help manage selection for herbicide resistant weed biotypes, potentially reduce the use of herbicides, and decrease potential adverse effects on non-target organisms [69]. However, from an eco-evolutionary perspective, the approach of "stacking" or "pyramiding" herbicidal control tactics implies increasing the dimensionality of the selection pressure by layering one selective force on the top of another [26].…”

Section: The Doomed Marriage Between Integrated Weed Management and Hmentioning

confidence: 99%

“…While few examples exist where local networks help famers coordinate actions to manage pests [84], management strategies, community outreach, and regulatory oversight should emphasize the need for landscape-level coordination of weed management. These guidelines should focus on spatio-temporally explicit and species-specific area-wide management plans [69,85], and mandate the maximum use of specific herbicide active ingredients or a resistant crop trait within a region. As an example, an empirical model predicted that a heavy infestation of C. canadensis in a single field could spread to tens to hundreds of surrounding farms [86].…”

Section: Concepts Actionsmentioning

confidence: 99%

The Eco-Evolutionary Imperative: Revisiting Weed Management in the Midst of an Herbicide Resistance Crisis

et al. 2016

View full text Add to dashboard Cite

Abstract:Modern weed science is at a crossroads. Born out of advances in chemistry, it has focused on minimizing weed competition with genetically uniform crops and heavy reliance on herbicides. Paradoxically, the success obtained with such an approach and the reluctance to conduct integrated and multidisciplinary research has resulted in unintended, but predictable, consequences, including the selection of herbicide resistant biotypes. Advances in eco-evolutionary biology, a relatively recent discipline that seeks to understand how local population dynamics arise from phenotypic variation resulting from natural selection, habitat distribution, and propagule dispersal across the landscape are transforming our understanding of the processes that regulate agroecosystems. Within this framework, complementary tactics to develop alternative weed management programs include the following: (1) weed scientists must recognize that evolution occurs within crop fields at ecologically-relevant time scales and is rooted in the inherent variation that exists in all populations; (2) weed management should recognize that the probability of a resistant mutant is directly related to the population size; (3) farmers need to acknowledge that herbicide resistance transcends any one farm and should coordinate local practices with regional actions; (4) incentives should be developed and implemented to help the adoption of eco-evolutionary management programs; and (5) risk analysis can help incorporate an eco-evolutionary perspective into integrated weed management programs.

show abstract

New Technologies for Insect-Resistant and Herbicide-Tolerant Plants

Cited by 65 publications

References 72 publications

Editing the Genome Without Double-Stranded DNA Breaks

Editing the Genome Without Double-Stranded DNA Breaks

Review. Development, Applications, Benefits, Challenges and Limitations of the New Genome Engineering Technique. An Update Study

The Eco-Evolutionary Imperative: Revisiting Weed Management in the Midst of an Herbicide Resistance Crisis

Contact Info

Product

Resources

About