New techniques for the assessment of canine semen quality: A review

Rijsselaere, Tom; Soom, Ann Van; Tanghe, Sofie; Coryn, M.; Maes, Dominiek; Kruif, Aart de

doi:10.1016/j.theriogenology.2005.05.021

Cited by 74 publications

(64 citation statements)

References 77 publications

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“…The ability to accurately evaluate the quality and estimate the fertilizing potential of dog semen has increased in importance as a result of the more widespread use of artificial insemination [1].…”

Section: Introductionmentioning

confidence: 99%

Validation of merocyanine 540 staining as a technique for assessing capacitation-related membrane destabilization of fresh dog sperm

Steckler¹,

Stout

Durandt

et al. 2015

Theriogenology

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The aim of this study was to determine whether flow cytometric evaluation of combined merocyanine 540 and Yo-Pro 1 staining (M540-YP) would identify viable dog sperm that had undergone membrane stabilization known to be associated with capacitation in other species, and whether such destabilization is detected earlier than when using the tyrosine phosphorylation and ethidium homodimer stain combination (TP-EH) with epifluorescence microscopy. Semen from nine dogs was collected and incubated in parallel in bicarbonate-free modified Tyrode"s medium (-BIC), in medium containing 15 mM bicarbonate (+BIC), in dog prostatic fluid (PF), and in phosphate buffered saline (PBS). Aliquots for staining were removed at various time points during incubation of up to 6 hours. Staining with M540-YP allowed the classification of dog sperm as viable without destabilized membranes, viable with 2 destabilized membranes, non-viable without destabilized membranes or non-viable with destabilized membranes. The percentage of viable sperm detected using EH (83.5 ± 1.37%; mean ± SEM) was higher than when using YP (66.7 ± 1.37%: P < 0.05; n=54 semen samples). On the other hand, M540-YP identified a higher percentage of viable sperm with destabilized membranes than TP-EH (75 ± 1.76% vs. capacitation was recorded during incubation in PBS. We conclude that YP identifies sperm committed to cell death earlier than EH, and that the M540-YP stain combination identifies membrane destabilization known to be associated with capacitation in other species earlier than the TP-EH stain combination.

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Section: Introductionmentioning

confidence: 99%

Validation of merocyanine 540 staining as a technique for assessing capacitation-related membrane destabilization of fresh dog sperm

Steckler¹,

Stout

Durandt

et al. 2015

Theriogenology

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“…Zona-binding tests aim to predict the fertilization ability of sperm, which is expected to be directly related to the fertilization potential of the male breeder. Results from this test are expected to be similar to those from in vivo fecundity tests given that they are capable of detecting sperm cell lesions at the molecular level, unlike conventional semen analysis tests [13]. Studies on humans and stallions have shown a positive correlation between zona-binding and male fertility [14], indicating that this test is possibly one of the most accurate in vitro techniques for semen analysis, given that it simulates an in vivo situation.…”

Section: Introductionmentioning

confidence: 88%

The Efficacy of In Vitro Sperm Tests in Predicting Pregnancy Success after Artificial Insemination in the Bitch

Icn¹,

A²,

Cr³

et al. 2017

Andrology

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In many species, including the dog (Canis familiaris), semen analysis protocols has improved in recent years. In vitro assessment of semen quality provides important information; however, the ultimate parameter for determining semen quality is pregnancy outcome. The objective of the present study was to investigate the efficiency of in vitro sperm tests in predicting seminal quality in dogs by correlating those results with pregnancy success after artificial insemination (AI). Forty-eight semen samples refrigerated for 24, 48 or 72 hours were examined for sperm motility and progressive motility, membrane integrity, and zona pellucida-binding capacity before being used to artificially inseminate bitches. Results indicated positive correlations between pregnancy success and sperm motility (0.77), vigor (0.72), membrane integrity (0.72) and zona pellucida-binding (0.81). Both pregnancy success and the sperm characteristics declined with increased semen storage time. In conclusion, the semen characteristics evaluated in this study can be used to predict the conception potential of semen samples in the dog.

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“…Membrane integrity of mammalian and avian spermatozoa may be assessed by using many fluorescent probe combinations including: carboxyfluorescein diacetate (CFDA) in combination with propidium iodide (PI), SYBR-14 with PI, carboxy-seminaphthorhodfluor (Carboxy-SNARF) with PI, calcein-AM with ethidium homodimer (EthD-1) and Hoechst 33258 (Christensen et al, 2004;Donoghue et al, 1995;Hewitt & England, 1998;Partyka et al, 2010;Peña et al, 1998;Rijsselaere et al, 2005;Sirivaidyapong et al, 2000). SYBR-14 and CFDA, usually used detectors of live cells, are membrane-permeant and non-fluorescent compounds, which are immediately deacylated and thus rapidly converted into high fluorescent compounds by intracellular esterases.…”

Section: Sperm Membrane Integritymentioning

confidence: 99%

“…The advantage of the use of fluorochromes is the possibility to assess the semen without the interference of fat particles and others material present in the extended semen (Rijsselaere et al, 2005). The detection of the third subpopulation i.e.…”

Section: Sperm Membrane Integritymentioning

confidence: 99%