New system for the detection of Legionella pneumophila in water samples

Párraga-Niño, Noemí; Quero, Sara; Ventós-Alfonso, Alba; Uría, Naroa; Castillo-Fernandez, Oscar; Ezenarro, Josune J.; Muñoz, F. Javier; García-Núñez, Marian; Sabrià, Miquel

doi:10.1016/j.talanta.2018.07.013

Cited by 21 publications

(20 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A concentration and detection protocol optimised and reported by our research group in a previous work was followed for the Legionella pneumophila detection [14]. Employing a custom-made holder, different inoculum between 10 1 and 10 5 cfu•mL −1 in a final volume of 200 mL of drinking water were filtered through a 25-mm diameter nitrocellulose (NC) membrane (Whatman Nitrocellulose, GE Healthcare Life Science, Buckinghamshire, UK) with a nominal pore of 0.2 µm at a flow rate of 0.5 mL•s −1 by a peristaltic pump (XX8000230, Merck Millipore, Burlington, VT, USA).…”

Section: Legionella Pneumophila Concentration and Antibody Reactionmentioning

confidence: 99%

“…Faster molecular methods such as PCR (polymerase chain reaction) have been developed, but these need highly skilled personnel, specific instrumentation, and are more costly [13]. Out of the 16 serogroups of Legionella pneumophila, serogroup 1 is responsible for the majority of the European and American isolates [14]. Some commercial kits have been developed for fast Legionella detection.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Rapid Detection of Legionella pneumophila in Drinking Water, Based on Filter Immunoassay and Chronoamperometric Measurement

et al. 2020

Self Cite

View full text Add to dashboard Cite

Legionella is a pathogenic bacterium, ubiquitous in freshwater environments and able to colonise man-made water systems from which it can be transmitted to humans during outbreaks. The prevention of such outbreaks requires a fast, low cost, automated and often portable detection system. In this work, we present a combination of sample concentration, immunoassay detection, and measurement by chronoamperometry. A nitrocellulose microfiltration membrane is used as support for both the water sample concentration and the Legionella immunodetection. The horseradish peroxidase enzymatic label of the antibodies permits using the redox substrate 3,3′,5,5′-Tetramethylbenzidine to generate current changes proportional to the bacterial concentration present in drinking water. Carbon screen-printed electrodes are employed in the chronoamperometric measurements. Our system reduces the detection time: from the 10 days required by the conventional culture-based methods, to 2–3 h, which could be crucial to avoid outbreaks. Additionally, the system shows a linear response (R2 value of 0.99), being able to detect a range of Legionella concentrations between 101 and 104 cfu·mL−1 with a detection limit (LoD) of 4 cfu·mL−1.

show abstract

Section: Legionella Pneumophila Concentration and Antibody Reactionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Rapid Detection of Legionella pneumophila in Drinking Water, Based on Filter Immunoassay and Chronoamperometric Measurement

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…When the light propagates through the detection channel filled with solution samples, the light intensity attenuates due to the absorption caused by sample molecules. The magnitude of such an attenuation is proportional to both the analyte concentration and the length of the optical pathway, which is described by the Beer-Lambert law 30 and expressed as eqn (1). This equation is the principle for quantitative absorbance analysis.…”

Section: Methodsmentioning

confidence: 99%

“…Absorbance measurement is a frequently used quantification method for various analysis applications such as immunoassays, 1,2 protein quantification, 3,4 detection of blood glucose, 5 assessment of environmental quality, [6][7][8] etc. Compared with absorbance analysis in 1 ml detection cells or 96-well plates, the microfluidic-based assay has attracted lots of attention due to the reduced sample consumption and the miniaturized system.…”

Section: Introductionmentioning

confidence: 99%

Self-aligned 3D microlenses in a chip fabricated with two-photon stereolithography for highly sensitive absorbance measurement

et al. 2020

View full text Add to dashboard Cite

show abstract

“…and Legionella pneumophila (L. pneumophila) in tap water samples involves Legiolert™/Quanti-Tray ® , providing acceptable accuracy [19]. Immunodetection is another proposed method, offering the advantage of fast detection [20]. One of the most frequently adopted culture-independent methods for Legionella detection and enumeration is PCR/qPCR.…”

Section: Introductionmentioning

confidence: 99%

Detection of pathogenic bacteria in hot tap water using the qPCR method: preliminary research

Wolf-Baca

Siedlecka

2019

SN Appl. Sci.

View full text Add to dashboard Cite

Drinking water should be safe for health and free from amounts of pathogenic microorganisms and parasites posing a threat to human health. In recent years, particular attention has been paid to the threat associated with the operation of hot water installations, potentially providing favourable conditions for the development of bacteria of genera Legionella and Escherichia. Such bacteria are commonly found in the natural environment, but should not reach the consumers' taps. In this experiment, hot water samples were collected from six public buildings, and the detection of pathogenic microorganisms, namely Legionella spp., Legionella pneumophila, and Escherichia coli bacteria, was performed by means of qPCR analysis. The sequences specific for these bacteria were quantified with TaqMan probes in total DNA extracts of the hot tap water samples. Bacteria of the Legionella spp. type were detected at five sampling sites in the range from 4.52 to 15.59 genomes/mL of hot tap water. Legionella pneumophila was detected at four sampling sites in the range from 0.98 to 11.99 genomes/mL. E. coli was recorded at four sites, but in quantities around 0.00 genomes/mL (less than one genome/mL). This preliminary research points to the need to use molecular techniques as an additional source of information in standard water analyses to obtain shorter detection time and more accurate results.

show abstract

New system for the detection of Legionella pneumophila in water samples

Cited by 21 publications

References 18 publications

Rapid Detection of Legionella pneumophila in Drinking Water, Based on Filter Immunoassay and Chronoamperometric Measurement

Rapid Detection of Legionella pneumophila in Drinking Water, Based on Filter Immunoassay and Chronoamperometric Measurement

Self-aligned 3D microlenses in a chip fabricated with two-photon stereolithography for highly sensitive absorbance measurement

Detection of pathogenic bacteria in hot tap water using the qPCR method: preliminary research

Contact Info

Product

Resources

About