New Synthetic Prosthesis for Peripheral Nerve Injuries

Uranüs, S.; Bretthauer, Georg; Nagele-Moser, D; Saliba, Sarah; Tomasch, G; Rafolt, D.; Justich, Ivo; Waldert, Jörg; Berghold, Andrea; Kleinert, R.; Becker, H.; Voges, Udo; Wiederstein-Grasser, Iris; Koch, Horst

doi:10.1177/1553350612458546

Cited by 6 publications

(5 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Larger animal models are ideal for simulating the long-distance nerve defects and regenerative phenomena that are observed in human PNI. Since these models can more reliably replicate some characteristics of human nerves, such as their structural composition, dimensions, diameter and regenerative process, there are already several published studies based on the use of dogs [16,17], cats [18], rabbits [19], non-human primates [20], pigs [13,21], mini-pigs [22], guinea-pigs [23], and sheep models [24].…”

Section: Introductionmentioning

confidence: 99%

Establishment of a Sheep Model for Hind Limb Peripheral Nerve Injury: Common Peroneal Nerve

Alvites

Branquinho

Sousa

et al. 2021

IJMS

View full text Add to dashboard Cite

Thousands of people worldwide suffer from peripheral nerve injuries and must deal daily with the resulting physiological and functional deficits. Recent advances in this field are still insufficient to guarantee adequate outcomes, and the development of new and compelling therapeutic options require the use of valid preclinical models that effectively replicate the characteristics and challenges associated with these injuries in humans. In this study, we established a sheep model for common peroneal nerve injuries that can be applied in preclinical research with the advantages associated with the use of large animal models. The anatomy of the common peroneal nerve and topographically related nerves, the functional consequences of its injury and a neurological examination directed at this nerve have been described. Furthermore, the surgical protocol for accessing the common peroneal nerve, the induction of different types of nerve damage and the application of possible therapeutic options were described. Finally, a preliminary morphological and stereological study was carried out to establish control values for the healthy common peroneal nerves regarding this animal model and to identify preliminary differences between therapeutic methods. This study allowed to define the described lateral incision as the best to access the common peroneal nerve, besides establishing 12 and 24 weeks as the minimum periods to study lesions of axonotmesis and neurotmesis, respectively, in this specie. The post-mortem evaluation of the harvested nerves allowed to register stereological values for healthy common peroneal nerves to be used as controls in future studies, and to establish preliminary values associated with the therapeutic performance of the different applied options, although limited by a small sample size, thus requiring further validation studies. Finally, this study demonstrated that the sheep is a valid model of peripheral nerve injury to be used in pre-clinical and translational works and to evaluate the efficacy and safety of nerve injury therapeutic options before its clinical application in humans and veterinary patients.

show abstract

Section: Introductionmentioning

confidence: 99%

Establishment of a Sheep Model for Hind Limb Peripheral Nerve Injury: Common Peroneal Nerve

Alvites

Branquinho

Sousa

et al. 2021

IJMS

View full text Add to dashboard Cite

show abstract

“…In the choice of materials for nerve guidance conduits, resorbability and biocompatibility, especially as reflected in non‐provocation of an inflammatory response, are of utmost importance and the gelatin we chose is optimal in this respect. No animal, either in the preliminary or the main study, showed any intolerance [15]. Our study leads to believe that the outer sheath of the conduit and the microtubules, both made of gelatin, provide a route for the budding and elongating axons to follow that mimics the natural structure of the neural pathway, with endoneural connective tissue covering the individual fascicles, and the individual nerve fibers being stabilized and protected from ingrowth of external connective tissue.…”

Section: Discussionmentioning

confidence: 79%

“…The conduit is composed of biocompatible absorbable gelatin [15]. The basic body is made of biocompatible absorbable gelatin with a length of 18 mm.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

A New Synthetic Conduit for the Treatment of Peripheral Nerve Injuries

et al. 2020

Self Cite

View full text Add to dashboard Cite

Background Peripheral nerve defects (PND) often cause lifelong physical disability, and the available treatment options are often not satisfactory. PND are usually bridged with an autologous nerve transplant or a nerve guidance conduit (NGC), when coaptation as preferred technique is not possible. The aim of this experimental study was to determine the effectiveness of a novel NGC for regeneration in the treatment of PND. Materials and methods A conduit made of gelatin with an innovative interior structure was tested for the repair of a 6-mm gap versus direct microsurgical suture repair without gap. Results We found that bridging the defect with this conduit was as effective as direct microsurgical coaptation without a defect. Conclusions This nerve conduit, effective in bridging neural defects, appears as an alternative to autologous nerve grafts, avoiding the problems related to nerve graft harvesting, host–donor differences in diameter, mismatches in number and pattern of fascicles, cross-sectional shape and area, and morbidity of the donor area.

show abstract

“…Larger animal models are ideal for simulating the long-distance nerve defects and regenerative phenomena that are observed in human PNI. Since these models can more reliably replicate some characteristics of human nerves, such as their structural composition, dimensions, diameter and regenerative process, there are already several published studies based on the use of dogs (29,30), cats (31), rabbits (32), non-human primates (33), pigs (26,34), mini-pigs (35), guinea-pigs (36), and sheep models (37).…”

Section: Introductionmentioning

confidence: 99%

Establishment of a Sheep Model for Hind limb Peripheral Nerve Injury: Common Peroneal Nerve

Alvites¹,

Branquinho²,

Sousa³

et al. 2020

Preprint

View full text Add to dashboard Cite

Thousands of people worldwide suffer from injuries in the peripheral nerve and deal daily with the resulting physiological and functional deficits. Recent advances in this field are still insufficient to guarantee effective outcomes, and the development of new and effective therapeutic options requires the use of valid preclinical models that effectively replicate the characteristics and challenges associated with these injuries in humans. In this study, we established a sheep model for common peroneal nerve injuries that can be applied in preclinical research with the advantages associated with the use of large animal models. In an integrative way, this article includes a detailed description of the anatomy and functionality of the peripheral nerves of sheep’s hind limb, the surgical protocol for accessing the common peroneal nerve, the induction of different types of nerve damage and the application of possible therapeutic options. A neurological exam protocol directed to the common peroneal nerve was also established, allowing to identify the changes and deficits related with the nerve injury and to evaluate the functional progression over time. Finally, a preliminary stereological study was carried out to establish control values for the healthy peroneal common nerves of this model and to identify preliminary differences between therapeutic methods. The ultimate goal is to demonstrate that sheep is a valid model of peripheral nerve injury to be used in pre-clinical and translational works and to evaluate the efficacy and safety of nerve injury therapeutic options before its clinical application in human and veterinary patients.

show abstract

New Synthetic Prosthesis for Peripheral Nerve Injuries

Cited by 6 publications

References 9 publications

Establishment of a Sheep Model for Hind Limb Peripheral Nerve Injury: Common Peroneal Nerve

Establishment of a Sheep Model for Hind Limb Peripheral Nerve Injury: Common Peroneal Nerve

A New Synthetic Conduit for the Treatment of Peripheral Nerve Injuries

Establishment of a Sheep Model for Hind limb Peripheral Nerve Injury: Common Peroneal Nerve

Contact Info

Product

Resources

About