New Strategies of LIBS-Based Validation of Glycemic Elements for Diabetes Management

Kumar, Prashant; Jaiswal, Dolly; Nilesh, K.; Pandhija, Shiwani; Shukla, V. K. S.; Watal, Geeta

doi:10.1007/s11483-009-9123-x

Cited by 8 publications

(5 citation statements)

References 18 publications

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“…29 Recently, we have found in our lab that Mg and Ca manage the blood glucose levels and also restore the antioxidant activity in type 2 diabetic rats. [30][31][32][33][34] From the outcome of this study, it can be conclusively stated that the higher concentrations of Ca ++ , Mg ++ , and Fe ++ , as reflected by their intensities, are responsible for glycemic potential of T. dioica. Further studies to establish the absolute concentrations of trace elements needed for optimum glycemic activity in diabetic management are in progress.…”

Section: Resultsmentioning

confidence: 58%

The Glycemic Elemental Profile of Trichosanthes dioica: A LIBS-Based Study

et al. 2009

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The scientific evaluation of the antidiabetic efficacy of aqueous extract of Trichosanthes dioica fruits on streptozotocin-induced diabetic rats is being presented. The graded doses of the extract, viz., 500, 750, 1,000, and 1,250 mg/kg body weight (bw), were administered orally, and it was observed that the blood glucose concentration decreased in a dose-dependent manner. The dose of 1,000 mg/kg bw showed the maximum fall of 23.8% and 19.1% in blood glucose level (BGL) during fasting BGL and glucose tolerance test (GTT) studies, respectively, of nondiabetic rats. Whereas in the case of subdiabetic and mild diabetic models, the same dose showed reduction in BGL of 22.0% and 31.4% during GTT. The study also involves the first use of laser-induced breakdown spectroscopy as a sensitive analytical tool to detect the elemental profile responsible for the antidiabetic activity of aqueous extract of T. dioica fruits that exhibits the antidiabetic activity. High intensities of Ca, Mg, and Fe indicate large concentrations of these elements in the extract, since according to Boltzmann's distribution law, intensities are directly proportional to concentrations. The higher concentrations of these glycemic elements, viz. Ca, Mg, and Fe, are responsible for the antidiabetic potential of T. dioica as well as other plant already reported by our research group.

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Section: Resultsmentioning

confidence: 58%

The Glycemic Elemental Profile of Trichosanthes dioica: A LIBS-Based Study

et al. 2009

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“…IR and UV spectra were recorded on Perkin-Elmer 599 B and Shimadzu UV-250 spectrometers respectively. 1 H NMR, 13 C NMR, spectra were recorded in DMSO-d 6 on Bruker 300 DPX, FT NMR ( 1 H: 400 MHz, 13 C: 100 MHz) spectrometers. Mass spectra were measured on a JEOL AX 500 spectrometer.…”

Section: Characterization Of Compoundsmentioning

confidence: 99%

“…Molecular formulas of both the unknown compounds were established by their elemental and spectral analysis viz. UV, IR, 1 H and 13 C NMR as follows: C-6΄ positions respectively. Furthermore, these spectral data are in agreement with the earlier reports of literature 5,16,17 .…”

Section: Isolation Of Compoundmentioning

confidence: 99%

“…Optical emission from the plasma consists of atomic and ionic spectral lines, characteristic of the constituent species, superimposed on a broad band continuum that is due to electron-ion recombination and free-free interactions. Identification of spectral lines and measurement of their intensities provides qualitative and quantitative information about the constituents of the target material [12,13]. In the present study the LIBS has been used for identifying the complete phytoelemental profile of Trichosanthes dioica responsible for its bioactivity.…”

Section: Introductionmentioning

confidence: 99%

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Therapeutic Phytoelemental Profile Of Trichosanthes Dioica

2010

Adv. Mater. Lett.

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In India, Trichosanthes dioica is known as pointed gourd (in English), parwal (in Hindi) and potol (in Oriya) it is distributed in equatorial and subtropical regions and widely found in northern India. The present study is a unique example of interdisciplinary research as it deals not only with the phytochemical investigation of Trichosanthes dioica, using natural product technology but also correlates the presence of certain trace elements with its biological activities using Laser induced break down Spectroscopy (LIBS). The study reveals the isolation of one known and two unknown flavonoids and the presence of certain glycemic elements, responsible for the observed antidiabetic activity of Trichosanthes dioica. Hence, the presence of isolated flavonoids and trace elements can be very well correlated with its medicinal value.

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“…Recently, Rai et al has also postulated the utility of LIBS technique on the plant product. 8,[12][13][14][15] Emblica officinalis (Family Euphorbiaceae) has been extensively used in traditional Indian system of medicine as an important constituent for scavenging reactive oxygen species. 16 The available reports indicated that different parts of E. officinalis have been used as anti-cancer, 17 antimutagenic, 18 antidiabetic, 19 hepatoprotective, 18 and also as antiulcer agents.…”

Section: Introductionmentioning

confidence: 99%

LIBS-Based Detection of Antioxidant Elements in Seeds of Emblica officinalis

Mehta

Kumar

et al. 2010

Food Biophysics

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The aim of the study was to determine the effect of the elements of the extract of seed from Emblica officinalis on antioxidant enzymes and osmotic fragility of erythrocytes membrane in normal as well as streptozotocininduced severely diabetic albino Wister rats. The results revealed that the untreated diabetic rats exhibited increase in oxidative stress as indicated by significantly diminished activities of free radical scavenging enzymes such as catalase (CAT) and superoxide dismutase (SOD) by 37.5% (p < 0.001) and 18.6% (p < 0.01), respectively. However, the E. officinalis seed extract treatment showed marked improvements in CAT and SOD activities by 47.09% (p<0.001) and 21.61% (p<0.001), respectively. The enhanced lipid peroxidation by 30.87% (p<0.001) in erythrocytes of untreated diabetic rats was significantly accentuated in the extract treated animals by 23.72% (p<0.001). The erythrocytes showed increased osmotic fragility due to diabetes in terms of hemolysis. It attained the normal level in diabetic treated group. The findings thus suggest that E. officinalis seed extract has the potential to be exploited as an agent to boost the antioxidant system in the diabetic animal model. Laser-induced breakdown spectroscopy has been used as an analytical tool to detect major and minor elements like Mg, Fe, Na, K, Zn, Ca, H, O, C, and N present in the extract. The higher concentration of Ca (II), Mg (II) and Fe (II) as reflected by their intensities are responsible for the antioxidant potential of E. officinalis.

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New Strategies of LIBS-Based Validation of Glycemic Elements for Diabetes Management

Cited by 8 publications

References 18 publications

The Glycemic Elemental Profile of Trichosanthes dioica: A LIBS-Based Study

The Glycemic Elemental Profile of Trichosanthes dioica: A LIBS-Based Study

Therapeutic Phytoelemental Profile Of Trichosanthes Dioica

LIBS-Based Detection of Antioxidant Elements in Seeds of Emblica officinalis

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