“…In adults, the use of partially T‐cell‐depleted haploidentical marrow using anti‐CD3 combined with intensive total body irradiation achieved similar results . With the establishment and availability of high‐quality monoclonal antibodies (MoAbs) developed for characterization of T‐cell subsets and hematopoietic (CD34+) progenitors, more effective positive and negative cell selection methods were established over the past two decades . Different cell selection systems were developed such as the Ceprate SC immunoaffinity column (CellPro, Bothell, WA), the Isolex 300i magnetic cell selection system (Nexell/Baxter, Irvine, CA), and the CliniMACS CD34 reagent system (Miltenyi, Cambridge, MA), Dynabeads (Dynal/Invitrogen, Carlsbad, CA) for immunopanning of target cells, and flow cytometric cell sorting techniques .…”