New Steroidal Glycosides from Mimusops elengi

Jahan, Nusrat; Ahmed, Wasim; Malik, Abdul

doi:10.1021/np50122a014

Cited by 44 publications

(34 citation statements)

References 8 publications

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“…The spectrometric analysis of CfC2F5 showed the presence of stigmast-7,22-dien-3-ol and stigmast-7-en-3-ol, respectively (Table1). The chemical structure of these phytosterols was confirmed by 1 H and 13 C NMR (Jahan et al, 1995;Seo et al, 1987). The CfC1F2 fraction (1.7 g) was again fractionated on a RP-18 silica gel column (10 g, 230-400 mesh, POLYGOPREP C18, Macherey-Nagel, Pennsylvania, USA), eluted with a gradient system of water: acetonitrile to give 49 fractions (CfC3F1-CfC3F49).…”

Section: Isolation and Identification Of Major Compounds From C Ficimentioning

confidence: 99%

Chemical Characterization of a Hypoglycemic Extract From Cucurbita Ficifolia Bouche That Induces Liver Glycogen Accumulation in Diabetic Mice.

Jessica¹,

Mario²,

Zamilpa³

et al. 2017

Afr J Tradit Complement Altern Med

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Background: The aqueous extract of Cucurbita ficifolia (C. ficifolia) fruit has demonstrated hypoglycemic effect, which may be attributed to some components in the extract. However, the major secondary metabolites in this fruit have not yet been identified and little is known about its extra-pancreatic action, in particular, on liver carbohydrate metabolism. Therefore, in addition to the isolation and structural elucidation of the principal components in the aqueous extract of C. ficifolia, the aim of this study was to determine whether or not the hypoglycemic effect of the aqueous extract of Cucurbita ficifolia (C. ficifolia) fruit is due to accumulation of liver glycogen in diabetic mice. Materials and Methods:The aqueous extract from fruit of C. ficifolia was fractionated and its main secondary metabolites were purified and chemically characterized (NMR and GC-MS). Alloxan-induced diabetic mice received daily by gavage the aqueous extract (30 days). The liver glycogen content was quantified by spectroscopic method and by PAS stain; ALT and AST by spectrometric method; glycogen synthase, glycogen phosphorylase and GLUT2 by Western blot; the mRNA expression of GLUT2 and glucagon-receptor by RT-PCR; while serum insulin was quantified by ELISA method. A liver histological analysis was also performed by H&E stain. Results: Chemical fingerprint showed five majoritarian compounds in the aqueous extract of C. ficifolia: p-coumaric acid, p-hydroxybenzoic acid, salicin, stigmast-7,2,2-dien-3-ol and stigmast-7-en-3-ol. The histological analysis showed accumulation of liver glycogen. Also, increased glycogen synthase and decreased glycogen phosphorylase were observed. Interestingly, the histological architecture evidenced a liver-protective effect due the extract. Conclusion: Five compounds were identified in C. ficifolia aqueous extract. The hypoglycemic effect of this extract may be partially explained by liver glycogen accumulation. The bioactive compound responsible for the hypoglycemic effect of this extract will be elucidated in subsequent studies.

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Section: Isolation and Identification Of Major Compounds From C Ficimentioning

confidence: 99%

Chemical Characterization of a Hypoglycemic Extract From Cucurbita Ficifolia Bouche That Induces Liver Glycogen Accumulation in Diabetic Mice.

Jessica¹,

Mario²,

Zamilpa³

et al. 2017

Afr J Tradit Complement Altern Med

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show abstract

“…[25] Several triterpenoids, steroids, steroidal glycosides, flavonoids, and alkaloids have been reported from this species. [25][26] The phytochemical review indicated the presence of taraxerol, taraxerone, ursolic acid, [27][28] betulinic acid, V-spinosterol, W-sitosterol, lupeol, [27][28] alkaloid isoretronecyl tiglate [29] and mixture of triterpenoid saponins in the bark of Mimusops elengi. In vitro free radical scavenging activity of methanol extract of the leaves of M. elengi was reported [30] in the literature.…”

Section: Preparation Of the Extractmentioning

confidence: 92%

“…[24] The bark and fruit of this plant are used in the treatment of diarrhea and dysentery, and a decoction of the bark is used as a gargle. [25] The pounded seeds pasted with oil are used for the treatment of obstinate constipation. Pillow stuffing made from the dried flowers induces nasal discharge and relieves headache.…”

Section: Preparation Of the Extractmentioning

confidence: 99%

Evaluation of in vitro Antioxidant Activity and Total Phenolic Content of Methanol Bark Extract of Mimusops elengi

Rao

Munjuluri

Kumar

et al. 2011

Free Radicals and Antioxidants

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“…The more polar fractions were purified by Sephadex LH-20 and RP-18 C.C., eluted with MeOH-H 2 O (90:10 and 85:15) to yield 1 (300 mg) and 2 (250 mg). The sugar residues as TMS derivative were identified by GC analysis using suitable sugar standard after acid hydrolysis [3] of the natural products.…”

Section: Introductionmentioning

confidence: 99%