New Star-Shaped Carriers Composed of β-Cyclodextrin Cores and Disulfide-Linked Poly(glycidyl methacrylate) Derivative Arms with Plentiful Flanking Secondary Amine and Hydroxyl Groups for Highly Efficient Gene Delivery

Hu, Yang; Zhu, Yun; Yang, Wan Tai; Xu, Fu‐Jian

doi:10.1021/am302249x

Cited by 59 publications

(74 citation statements)

References 35 publications

(86 reference statements)

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“…This confirms superparamagnetic behavior of the as-prepared nanoparticles at 300 K. The nano-composite with different lengths of PGMA chain segments has been synthesized by varying the ATRP time. 33 In addition, the ligand to transition metal ratio is an important parameters in the AGET ATRP process. This is because it has a strong effect on the polymerization rate and the level of control attained during the polymerization.…”

Section: Synthesis Of Fe 3 O 4 @Sio 2 -G-pgma Hybrid Nanoparticlesmentioning

confidence: 99%

Preparation and characterization of poly(glycidyl methacrylate)-grafted magnetic nanoparticles: Effects of the precursor concentration on polyol synthesis of Fe3O4 and [PMDETA]0/[CuBr2]0 ratios on SI-AGET ATRP

Jiang

Zhou

Wei

et al. 2015

Applied Surface Science

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Section: Synthesis Of Fe 3 O 4 @Sio 2 -G-pgma Hybrid Nanoparticlesmentioning

confidence: 99%

Preparation and characterization of poly(glycidyl methacrylate)-grafted magnetic nanoparticles: Effects of the precursor concentration on polyol synthesis of Fe3O4 and [PMDETA]0/[CuBr2]0 ratios on SI-AGET ATRP

Jiang

Zhou

Wei

et al. 2015

Applied Surface Science

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“…16,17 Our group recently discovered that ethanolamine (EA)-functionalized poly(glycidyl methacrylate) (PGMA) (denoted as PGEA) possessed good gene delivery behavior in various cells. 17−19 PGEA owns massive flanking hydroxyl groups for eliminating the adverse effect of extra charges, making PGEA to be an excellent candidate for fabricating lowtoxic vectors.…”

Section: ■ Introductionmentioning

confidence: 99%

Acid-Labile Poly(glycidyl methacrylate)-Based Star Gene Vectors

Yang

Wang

et al. 2015

ACS Appl. Mater. Interfaces

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It was recently reported that ethanolamine-functionalized poly(glycidyl methacrylate) (PGEA) possesses great potential applications in gene therapy due to its good biocompatibility and high transfection efficiency. Importing responsivity into PGEA vectors would further improve their performances. Herein, a series of responsive star-shaped vectors, acetaled β-cyclodextrin-PGEAs (A-CD-PGEAs) consisting of a β-CD core and five PGEA arms linked by acid-labile acetal groups, were proposed and characterized as therapeutic pDNA vectors. The A-CD-PGEAs owned abundant hydroxyl groups to shield extra positive charges of A-CD-PGEAs/pDNA complexes, and the star structure could decrease charge density. The incorporation of acetal linkers endowed A-CD-PGEAs with pH responsivity and degradation. In weakly acidic endosome, the broken acetal linkers resulted in decomposition of A-CD-PGEAs and morphological transformation of A-CD-PGEAs/pDNA complexes, lowering cytotoxicity and accelerating release of pDNA. In comparison with control CD-PGEAs without acetal linkers, A-CD-PGEAs exhibited significantly better transfection performances.

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“…12,13 Recently, we reported that ethanolamine (EA)-functionalized poly(glycidyl methacrylate) (PGMA), namely PGEA, has abundant hydroxyl and amino groups, and exhibited good gene transfection performances. 17,18 Boronic acid (BA)-decorated materials had been extensively applied into the biomedical fields because of the reversible formation of five-or six-member cyclic ester between the boronic acid and cis 1,2-or 1,3-diols. 17,18 Boronic acid (BA)-decorated materials had been extensively applied into the biomedical fields because of the reversible formation of five-or six-member cyclic ester between the boronic acid and cis 1,2-or 1,3-diols.…”

Section: Introductionmentioning

confidence: 99%

PGMA-based starlike polycations with flanking phenylboronic acid groups for highly efficient multifunctional gene delivery systems

Song

2015

Polym. Chem.

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Phenylboronic acid (PBA) possesses good affinities to glycoproteins on cell surfaces, as well as stable fluorescence properties. Recently, it was found that ethanolamine (EA)-decorated poly(glycidyl methacrylate) (PGMA), namely PGEA, exhibited promising applications as effective gene vectors. In this work, a strategy to prepare series of starlike PGEA vectors (s-PGEA-B) with flanking PBA groups was proposed for the development of high-efficient multifunctional gene delivery systems. Compared with the s-PGEA vectors without PBA groups, the corresponding s-PGEA-B counterparts showed the greater ability to bind pDNA. The sizes of the formed s-PGEA-B/pDNA nanoparticles were ~100 nm at higher N/P ratios. The s-PGEA-B/pDNA complexes exhibited enhanced cellular internalization and transfection efficiencies, particularly in the cell lines with abundant cell surface glycoproteins. The cellular internalization mediated by s-PGEA-B with 30% of PBA content was about 85%, much higher than that (about 53%) of the corresponding s-PGEA in HepG2 cells. In addition, the simultaneous fluorescence emitted from s-PGEA-B provided convenient observation for locating gene vectors during the transfection processes. This present work would provide new useful information for the design of multifunctional gene delivery systems. 16increasing amounts of polycations helped to condense pDNA at moderate N/P ratios, while at higher N/P ratios the excess polycations did not participate in complexing.Compared with s-PGEA, the corresponding s-PGEA-B exhibited stronger ability to compress pDNA into smaller nanoparticles particularly at the low N/P ratios, further confirming that the introduction of PBA groups contributed to condense pDNA. Fig. 3(b) shows the corresponding zeta potentials of the complexes at the N/P ratios from 5 to 35. At the low N/P ratio of 5, the ζ-potentials of s-PGEA-B/pDNA complexes were slightly higher than those of the corresponding s-PGEA/pDNA complexes, probably due to the stronger condensation ability of s-PGEA-B. However, at the higher N/P ratios, particularly N/P ratio of 30, s-PGEA-B/pDNA complexes exhibited the moderately lower ζ-potentials than the corresponding s-PGEA/pDNA complexes. As mentioned above, at higher N/P ratios the excess s-PGEA-B and s-PGEA polycations did not participate in complexing. The amount increase of weakly negative-charged PBA groups may fairly decrease the ζ-potentials of complex surfaces. The still positive ζ-potentials would make the s-PGEA-B/pDNA complexes possess good affinity to the negative-charged cell membranes. The visualized morphologies of complexes were presented in Fig. 4. The representative AFM images of s-PGEA1/pDNA and s-PGEA1-B2/pDNA complexes revealed that the polycations condensed pDNA into uniform nanospheres with the diameter of around 100 nm.

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New Star-Shaped Carriers Composed of β-Cyclodextrin Cores and Disulfide-Linked Poly(glycidyl methacrylate) Derivative Arms with Plentiful Flanking Secondary Amine and Hydroxyl Groups for Highly Efficient Gene Delivery

Cited by 59 publications

References 35 publications

Preparation and characterization of poly(glycidyl methacrylate)-grafted magnetic nanoparticles: Effects of the precursor concentration on polyol synthesis of Fe3O4 and [PMDETA]0/[CuBr2]0 ratios on SI-AGET ATRP

Preparation and characterization of poly(glycidyl methacrylate)-grafted magnetic nanoparticles: Effects of the precursor concentration on polyol synthesis of Fe3O4 and [PMDETA]0/[CuBr2]0 ratios on SI-AGET ATRP

Acid-Labile Poly(glycidyl methacrylate)-Based Star Gene Vectors

PGMA-based starlike polycations with flanking phenylboronic acid groups for highly efficient multifunctional gene delivery systems

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