New spectrophotometric assay for assessments of catalase activity in biological samples

Hadwan, Mahmoud Hussein; Ali, Seenaa Kadhum

doi:10.1016/j.ab.2017.11.013

Cited by 63 publications

(35 citation statements)

References 26 publications

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“…Hadwan and Ali [10]. In agreement with their findings, our results (Table 7) showed that Staphylococcus aureus has higher catalase enzyme activity than other types of bacteria.…”

Section: 7supporting

confidence: 91%

“…homogenized red blood cells as a source of catalase activity. The disubstituted quinone method was used to evaluate catalase enzyme activity, and the results were compared with those using the peroxovanadate protocol, as described by Hadwan and Ali [10].…”

Section: Concentration Of Chemicalmentioning

confidence: 99%

“…Spectrophotometric methods have widely been used to estimate catalase enzyme activity, using diverse reagents to form colored complexes that absorb light within the visible spectrum, such as the ferrous oxidation in xylenol orange (FOX) assay [7] that absorbs at 560 nm, the carbonate cobaltate(III) complex that absorbs light at 440 nm [8], the indamine dye with a water-soluble ironporphyrin [9] that absorb at 590 nm, and the peroxovanadate complex (NH4[VO(O2)SO4) that absorbs light at 452 nm [10]. Shivakumar et al, [11] utilized an isonicotinic acid hydrazidepyrocatechol system to examine the dissociation of hydrogen peroxide and assess catalase activity.…”

Section: Introductionmentioning

confidence: 99%

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New Spectrophotometric Method for the Assessment of Catalase Enzyme Activity in Biological Tissues

Hamza¹,

Hadwan²

2019

Preprint

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Background: Catalase is a vital antioxidant enzyme that dismutates H2O2 into water and molecular oxygen. Many protocols have been developed to measure catalase enzyme activity. Spectrophotometric methods are the most common assays that used to assess catalase enzyme activity. Methods: Because the rate-limiting step during catalase enzyme activity depends upon the dissociation of hydrogen peroxide, the developed assay measures the reaction between a hydroquinone/anilinium sulfate/ammonium molybdate reagent and Unreacted Hydrogen Peroxide, which results in the production of a purple, disubstituted quinone compound with a maximum absorbance value at 550 nm. Results: To clarify the precision of the developed method, the coefficients of variation were determined to be 2.6% and 4.7% for within run measurements and between run measurements, respectively. This method returned results that correlated well (r = 0.9982) with the results returned using the peroxovanadate method to assess catalase enzyme activity. Additionally, we examined the use of the newly developed hydroquinone assay to measure catalase enzyme activity in liver and bacterial homogenate samples. Conclusion: These results demonstrated that this assay can be used for scientific research and routine health applications because it is inexpensive, simple, accurate, and rapid. This method is suitable for use in clinical pathology laboratories because it is simple and produces precise and reproducible results.

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“…Hadwan and Ali [10]. In agreement with their findings, our results (Table 7) showed that Staphylococcus aureus has higher catalase enzyme activity than other types of bacteria.…”

Section: 7supporting

confidence: 91%

Section: Concentration Of Chemicalmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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New Spectrophotometric Method for the Assessment of Catalase Enzyme Activity in Biological Tissues

Hamza¹,

Hadwan²

2019

Preprint

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“…Catalase (CAT) activity was measured at 37 °C, according to Hadwan and Ali [36]. For this, ammonium vanadate reacts with hydrogen peroxide in an acidic solution, forming a peroxovanadium complex which is inversely proportional to CAT activity.…”

Section: Methodsmentioning

confidence: 99%

Spinal Reactive Oxygen Species and Oxidative Damage Mediate Chronic Pain in Lame Dairy Cows

Herzberg

Strobel

Chihuailaf

et al. 2019

Animals

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Simple SummaryChronic inflammatory diseases could impact central nervous system homeostasis, being oxidative damage of the dorsal horn, a relevant mechanism mediating central sensitization. Chronic inflammatory lameness in dairy cows is a painful condition that affects animal welfare, affecting dairy production worldwide. This study reveals increased levels of reactive oxygen species, malondialdehyde, and carbonyl groups, indicating lipid and protein damage in the spinal cord of cows with chronic lameness. Moreover, antioxidant system activity was similar between lame and non-lame cows which suggests that antioxidant dysregulation was not the cause of oxidative damage, as has been proposed previously. Based on the fact that nociceptive pathways are strongly conserved between species, there is no reason to neglect that chronic pain in cows promotes Central Nervous System (CNS) alterations, such as oxidative damage. Moreover, lame cows develop central sensitization, as allodynia and hyperalgesia are centrally and not peripherally mediated. Our results support the current assumption that chronic pain is a central nervous system disease and lameness in dairy cows is far beyond an inflammation of the hoof.AbstractLameness in dairy cows is a worldwide prevalent disease with a negative impact on animal welfare and herd economy. Oxidative damage and antioxidant system dysfunction are common features of many CNS diseases, including chronic pain. The aim of this study was to evaluate the levels of reactive oxygen species (ROS) and oxidative damage markers in the spinal cord of dairy cows with chronic inflammatory lameness. Locomotion score was performed in order to select cows with chronic lameness. Dorsal horn spinal cord samples were obtained post mortem from lumbar segments (L2–L5), and ROS, malondialdehyde (MDA), and carbonyl groups were measured along with the activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and total antioxidant response (TAR). Lame cows had increased levels of ROS, MDA, and carbonyl groups, while no differences were observed between lame and non-lame cows in SOD, GPx, CAT, and TAR activity. We conclude that painful chronic inflammatory lameness in dairy cows is associated with an increase in ROS, MDA, and carbonyl groups. Nonetheless, an association between ROS generation and dysfunction of the antioxidant system, as previously proposed, could not be established.

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“…The range of vitamin B12 was determined by the microbiological techniques with the Escherichia coli strains 115-6115-6 (Bandeli and Tuschhoff, 1954). The "Shimadzu UV-1700 series" spectrophotometer was used to determine the range of conjugated dienes, the primary products of lipid peroxidation (Pryor and Castle, 1984), malonic dialdehyde (Kazimirskii et al, 2018), catalase activity (Hadwan and Ali, 2018), superoxide dismutase (Guemouri et al, 1991), glutathione peroxidase (Paglia and Valentine, 1967) and erythrocyte acid resistance (Pokrovskii and Abrarov, 1964).…”

Section: Biochemical Analysismentioning

confidence: 99%

Evaluation of Hematological and Metabolic Parameters in Small Ruminants with Trace Elements Deficiency under Different Biogeochemical Conditions.

Vorobyov¹,

Vorobyov²,

Polkovnichenko³

et al. 2019

jwpr

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In the present study soil, water, pasture plants, organs and tissues of crossbred sheep of the Soviet Aksaray and Zaanen German White Improved goats were analyzed for their Selenium (Se), Iodine (I), Cobalt (Co), zinc, copper, and manganese content in the Lower Volga region. The biogeochemical situation of terrestrial ecosystems of the Lower Volga region was characterized by Se, Co, and I deficiencies in soil, water, pasture plants, and feed of crossbred sheep of the Soviet Aksaray and Zaanen German White Improved goats. The deficiency of these trace elements in small ruminants had been compensated by changes in hematological parameters include high Red Blood Cell (RBC) and White Blood Cell (WBC) and biochemical parameters. Meanwhile, the analyzed trace elements in the organs and tissues of crossbred sheep (n = 6) and Zaanen German white improved goats (n = 6) demonstrated that goats had lower amounts of Se (0.0136 ± 0.002 mg/kg), I (0.19 ± 0.01mg/kg), and Co (0619 ± 0.03 mg/kg) compared to sheep. The animals were recorded with a decrease in alkali reserve, the content of total protein and lipids, vitamins A, E, C, B12, total calcium, and inorganic phosphorus, increase in glucose, conjugated dienes and malonic di-aldehyde in the blood, and functional insufficiency of the antioxidant protection system.

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New spectrophotometric assay for assessments of catalase activity in biological samples

Cited by 63 publications

References 26 publications

New Spectrophotometric Method for the Assessment of Catalase Enzyme Activity in Biological Tissues

New Spectrophotometric Method for the Assessment of Catalase Enzyme Activity in Biological Tissues

Spinal Reactive Oxygen Species and Oxidative Damage Mediate Chronic Pain in Lame Dairy Cows

Evaluation of Hematological and Metabolic Parameters in Small Ruminants with Trace Elements Deficiency under Different Biogeochemical Conditions.

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